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Article

Systematic Analysis of the Pleurotus ostreatus Laccase Gene (PoLac) Family and Functional Characterization of PoLac2 Involved in the Degradation of Cotton-Straw Lignin

by
Xiaoyu Jiao
1,†,
Guoqing Li
1,2,†,
Yan Wang
1,
Fan Nie
2,
Xi Cheng
1,
Muhammad Abdullah
1,
Yi Lin
1 and
Yongping Cai
1,*
1
School of Life Sciences, Anhui Agricultural University, No. 130, Changjiang West Road, Hefei 230036, China
2
Horticultural Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2018, 23(4), 880; https://doi.org/10.3390/molecules23040880
Submission received: 16 March 2018 / Revised: 6 April 2018 / Accepted: 7 April 2018 / Published: 11 April 2018
(This article belongs to the Section Metabolites)
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Abstract

Fungal laccases play important roles in the degradation of lignocellulose. Although some PoLacs have been reported in several studies, still no comprehensive bioinformatics study of the LAC family in Pleurotus ostreatus has been reported. In this study, we identified 12 laccase genes in the whole genome sequence of P. ostreatus and their physical characteristics, gene distribution, phylogenic relationships, gene structure, conserved motifs, and cis-elements were also analyzed. The expression patterns of 12 PoLac genes at different developmental stages and under different culture substrates were also analyzed. The results revealed that PoLac2 and PoLac12 may be involved in the degradation of lignin and the formation of the fruiting body, respectively. Subsequently, we overexpressed PoLac2 in P. ostreatus by the Agrobacterium tumefaciens-mediated transformation (ATMT) method. The transformants’ laccase activity increased in varying degrees, and the gene expression level of PoLac2 in transformants was 2–8 times higher than that of the wild-type strain. Furthermore, the lignin degradation rate by transgenic fungus over 30 days was 2.36–6.3% higher than that of wild-type. Our data show that overexpression of PoLac2 significantly enhanced the lignin degradation of cotton-straw. To our knowledge, this study is the first report to demonstrate the functions of PoLac2 in P. ostreatus.
Keywords: Pleurotus ostreatus; laccase gene; phylogenetic analysis; expression profiling; overexpression; lignin degradation Pleurotus ostreatus; laccase gene; phylogenetic analysis; expression profiling; overexpression; lignin degradation
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MDPI and ACS Style

Jiao, X.; Li, G.; Wang, Y.; Nie, F.; Cheng, X.; Abdullah, M.; Lin, Y.; Cai, Y. Systematic Analysis of the Pleurotus ostreatus Laccase Gene (PoLac) Family and Functional Characterization of PoLac2 Involved in the Degradation of Cotton-Straw Lignin. Molecules 2018, 23, 880. https://doi.org/10.3390/molecules23040880

AMA Style

Jiao X, Li G, Wang Y, Nie F, Cheng X, Abdullah M, Lin Y, Cai Y. Systematic Analysis of the Pleurotus ostreatus Laccase Gene (PoLac) Family and Functional Characterization of PoLac2 Involved in the Degradation of Cotton-Straw Lignin. Molecules. 2018; 23(4):880. https://doi.org/10.3390/molecules23040880

Chicago/Turabian Style

Jiao, Xiaoyu, Guoqing Li, Yan Wang, Fan Nie, Xi Cheng, Muhammad Abdullah, Yi Lin, and Yongping Cai. 2018. "Systematic Analysis of the Pleurotus ostreatus Laccase Gene (PoLac) Family and Functional Characterization of PoLac2 Involved in the Degradation of Cotton-Straw Lignin" Molecules 23, no. 4: 880. https://doi.org/10.3390/molecules23040880

APA Style

Jiao, X., Li, G., Wang, Y., Nie, F., Cheng, X., Abdullah, M., Lin, Y., & Cai, Y. (2018). Systematic Analysis of the Pleurotus ostreatus Laccase Gene (PoLac) Family and Functional Characterization of PoLac2 Involved in the Degradation of Cotton-Straw Lignin. Molecules, 23(4), 880. https://doi.org/10.3390/molecules23040880

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