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Molecules 2018, 23(4), 737;

The Melatonin Signaling Pathway in a Long-Term Memory In Vitro Study

Department of Medical Genetics, Hanvit Institutute for Medical Genetics, School of Medicine, Keimyung University, Daegu 42601, Korea
Department of Biomedical Engineering, School of Medicine, Keimyung University, Daegu 42601, Korea
Dongsan Medical Center, Department of Internal Medicine, Keimyung University, Daegu 42931, Korea
Author to whom correspondence should be addressed.
Academic Editor: Dun-Xian Tan
Received: 30 January 2018 / Revised: 15 March 2018 / Accepted: 20 March 2018 / Published: 23 March 2018
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The activation of cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB) via phosphorylation in the hippocampus is an important signaling mechanism for enhancing memory processing. Although melatonin is known to increase CREB expression in various animal models, the signaling mechanism between melatonin and CREB has been unknown in vitro. Thus, we confirmed the signaling pathway between the melatonin receptor 1 (MT1) and CREB using melatonin in HT-22 cells. Melatonin increased MT1 and gradually induced signals associated with long-term memory processing through phosphorylation of Raf, ERK, p90RSK, CREB, and BDNF expression. We also confirmed that the calcium, JNK, and AKT pathways were not involved in this signaling pathway by melatonin in HT-22 cells. Furthermore, we investigated whether melatonin regulated the expressions of CREB-BDNF associated with long-term memory processing in aged HT-22 cells. In conclusion, melatonin mediated the MT1-ERK-p90RSK-CREB-BDNF signaling pathway in the in vitro long-term memory processing model and increased the levels of p-CREB and BDNF expression in melatonin-treated cells compared to untreated HT-22 cells in the cellular aged state. Therefore, this paper suggests that melatonin induces CREB signaling pathways associated with long-term memory processing in vitro. View Full-Text
Keywords: melatonin; long-term memory; CREB signal; cellular senescence melatonin; long-term memory; CREB signal; cellular senescence

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Sung, J.-Y.; Bae, J.-H.; Lee, J.-H.; Kim, Y.-N.; Kim, D.-K. The Melatonin Signaling Pathway in a Long-Term Memory In Vitro Study. Molecules 2018, 23, 737.

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