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Open AccessArticle

Determination of Milk Products in Ceramic Vessels of Corded Ware Culture from a Late Eneolithic Burial

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Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. listopadu 12, 779 00 Olomouc, Czech Republic
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Archaeological Centre Olomouc, U Hradiska 42/6, 779 00 Olomouc, Czech Republic
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Institute of Archaeological Heritage Brno, Kaloudova 1321/30, 614 00 Brno, Czech Republic
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Centre of Biology, Geoscience and Environmental Education, University of West Bohemia, Sedláčkova 15, 30614 Plzeň, Czech Republic
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Laboratory of Archaeobotany and Palaeoecology, Faculty of Science, University of South Bohemia, Na Zlaté stoce 3, 370 05 České Budějovice, Czech Republic
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Laboratory of Morphology and Forensic Anthropology (LaMorFA), Department of Anthropology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic
*
Author to whom correspondence should be addressed.
Molecules 2018, 23(12), 3247; https://doi.org/10.3390/molecules23123247
Received: 14 November 2018 / Revised: 29 November 2018 / Accepted: 4 December 2018 / Published: 7 December 2018
(This article belongs to the Section Analytical Chemistry)
In this study, a soil from two ceramic vessels belonging to Corded Ware culture, 2707–2571 B.C., found in a cremation grave discovered in Central Moravia, Czech Republic, was analyzed using matrix-assisted laser desorption/ionization–mass spectrometry (MALDI–MS) combined with advanced statistical treatment (principal component analysis, PCA, and orthogonal projection to latent structures discriminant analysis, OPLS-DA) and by enzyme-linked immunosorbent assay (ELISA). MALDI–MS revealed the presence of triacylglycerols in both vessels. This analytical technique was used for the analysis of the soil content from archaeological ceramic vessels for the first time. Targeted ELISA experiments consequently proved the presence of milk proteins in both ceramic vessels. These results represent the first direct evidence of the use of milk or dairy products in the Eneolithic period in Moravian Corded Ware Culture and help to better understand the diet habits and living conditions of Eneolithic populations in Central Europe. View Full-Text
Keywords: ceramic vessels; laser desorption–ionization; mass spectrometry; milk; enzyme-linked immunosorbent assay; Eneolithic period; Corded Ware culture ceramic vessels; laser desorption–ionization; mass spectrometry; milk; enzyme-linked immunosorbent assay; Eneolithic period; Corded Ware culture
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Kučera, L.; Peška, J.; Fojtík, P.; Barták, P.; Sokolovská, D.; Pavelka, J.; Komárková, V.; Beneš, J.; Polcerová, L.; Králík, M.; Bednář, P. Determination of Milk Products in Ceramic Vessels of Corded Ware Culture from a Late Eneolithic Burial. Molecules 2018, 23, 3247.

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