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Molecules 2018, 23(12), 3145; https://doi.org/10.3390/molecules23123145

Selection of Specific Peptides for Coccidioides spp. Obtained from Antigenic Fractions through SDS-PAGE and Western Blot Methods by the Recognition of Sera from Patients with Coccidioidomycosis

1
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Ciudad Universitaria No. 3000, México Cd. Mx. 04510, Mexico
2
Hospital Regional de Alta Especialidad de Ixtapaluca, Carretera Federal México—Puebla Km. 34.5, Pueblo de Zoquiapan, Ixtapaluca 56530, Mexico
3
Unidad de Servicios de Apoyo a la Investigación y la Industria (USAII), Facultad de Química, Universidad Nacional Autónoma de México (UNAM), Ciudad Universitaria No. 3000, México Cd. Mx. 04510, Mexico
4
Instituto Nacional de Enfermedades Respiratorias (INER), Calzada de Tlalpan 4502, Belisario Domínguez Sección XVI, Tlalpan, México Cd. Mx 14080, Mexico
*
Author to whom correspondence should be addressed.
Received: 24 October 2018 / Revised: 26 November 2018 / Accepted: 29 November 2018 / Published: 30 November 2018
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Abstract

Antigenic fractions of 100, 50, 37, and 28 kDa obtained through the SDS-PAGE method that were more frequently recognized by anti-Coccidioides antibodies in the sera of coccidioidomycosis patients were selected using western blotting. Subsequently, these bands were sequenced, and the obtained proteins were analysed by BLAST to choose peptides specific for Coccidioides spp. from among the shared aligned sequences of related fungi. A peptide specific for C. immitis was selected from the “GPI anchored serine-threonine rich protein OS C. immitis”, while from the “uncharacterized protein of C. immitis”, we selected a peptide for C. immitis and C. posadasii. These proteins arose from the 100 kDa antigenic fraction. From the protein “fatty acid amide hydrolase 1 of C. posadasii” that was identified from the 50 kDa antigenic fraction, a peptide was selected that recognized C. immitis and C. posadasii. In addition, the analysis of all the peptides (353) of each of the assembled proteins showed that only 35 had 100% identity with proteins of C. immitis and C. posadasii, one had 100% identity with only C. immitis, and one had 100% identity with only C. posadasii. These peptides can be used as diagnostic reagents, vaccines, and antifungals. View Full-Text
Keywords: coccidioidomycosis; immunodiagnosis; peptides; SDS-PAGE; Coccidioides spp. coccidioidomycosis; immunodiagnosis; peptides; SDS-PAGE; Coccidioides spp.
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Duarte Escalante, E.; Frías De León, M.G.; Martínez García, L.G.; Herrera, J.; Acosta Altamirano, G.; Cabello, C.; Palma, G.; Reyes Montes, M.R. Selection of Specific Peptides for Coccidioides spp. Obtained from Antigenic Fractions through SDS-PAGE and Western Blot Methods by the Recognition of Sera from Patients with Coccidioidomycosis. Molecules 2018, 23, 3145.

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