Next Article in Journal
Galactosylated Chitosan-Functionalized Mesoporous Silica Nanoparticle Loading by Calcium Leucovorin for Colon Cancer Cell-Targeted Drug Delivery
Next Article in Special Issue
Time-Resolved Spectroscopic and Density Functional Theory Investigation of the Photogeneration of a Bifunctional Quinone Methide in Neutral and Basic Aqueous Solutions
Previous Article in Journal
Use of Red Wine Polyphenols as a Natural Preservative in Health-Promoting Omega-3 Fatty Acids-Enriched Lamb Patties
Previous Article in Special Issue
Anti-Cancer Drug Sensitivity Assay with Quantitative Heterogeneity Testing Using Single-Cell Raman Spectroscopy
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessArticle
Molecules 2018, 23(12), 3081; https://doi.org/10.3390/molecules23123081

Raman Spectroscopic Analysis to Detect Reduced Bone Quality after Sciatic Neurectomy in Mice

1
Department of Bone and Joint Surgery, Ehime University Graduate School of Medicine, Shitsukawa, Toon 791-0295, Ehime, Japan
2
Biomedical Optics Laboratory, Graduate School of Biomedical Engineering Tohoku University, Aramaki Aza Aoba, Aoba-ku, Sendai 980-8579, Miyagi, Japan
3
Department of Gastroenterological and Pediatric Surgery, Oita University Faculty of Medicine, Idaigaoka, Hasama-machi, Yufu City 879-5593, Oita, Japan
4
Oral-Maxillofacial Surgery and Orthodontics, University of Tokyo Hospital, 7-3-1 Hongo, Bunkyo-ku 113-8655, Tokyo, Japan
5
Division of Integrative Pathophysiology, Proteo-Science Center, Ehime University, Shitsukawa, Toon 791-0295, Ehime, Japan
6
Division of Bio-imaging, Proteo-Science Center, Ehime university graduate school of medicine, Shitsukawa, Toon 791-0295, Ehime, Japan
7
Division of Analytical Bio-Medicine, Advanced Research Support Center, Ehime University, Shitsukawa, Toon 791-0295, Ehime, Japan
8
Molecular Medicine for Pathogenesis, Ehime university graduate school of medicine, Shitsukawa, Toon 791-0295, Ehime, Japan
*
Author to whom correspondence should be addressed.
Received: 23 October 2018 / Revised: 13 November 2018 / Accepted: 24 November 2018 / Published: 25 November 2018
(This article belongs to the Special Issue Raman Spectroscopy: A Spectroscopic 'Swiss-Army Knife')
Full-Text   |   PDF [2649 KB, uploaded 27 November 2018]   |  
  |   Review Reports

Abstract

Bone mineral density (BMD) is a commonly used diagnostic indicator for bone fracture risk in osteoporosis. Along with low BMD, bone fragility accounts for reduced bone quality in addition to low BMD, but there is no diagnostic method to directly assess the bone quality. In this study, we investigated changes in bone quality using the Raman spectroscopic technique. Sciatic neurectomy (NX) was performed in male C57/BL6J mice (NX group) as a model of disuse osteoporosis, and sham surgery was used as an experimental control (Sham group). Eight months after surgery, we acquired Raman spectral data from the anterior cortical surface of the proximal tibia. We also performed a BMD measurement and micro-CT measurement to investigate the pathogenesis of osteoporosis. Quantitative analysis based on the Raman peak intensities showed that the carbonate/phosphate ratio and the mineral/matrix ratio were significantly higher in the NX group than in the Sham group. There was direct evidence of alterations in the mineral content associated with mechanical properties of bone. To fully understand the spectral changes, we performed principal component analysis of the spectral dataset, focusing on the matrix content. In conclusion, Raman spectroscopy provides reliable information on chemical changes in both mineral and matrix contents, and it also identifies possible mechanisms of disuse osteoporosis. View Full-Text
Keywords: osteoporosis; bone quality; Raman spectroscopy; sciatic neurectomy; hydroxyapatite; mineral maturity; bone matrix; collagen crosslink osteoporosis; bone quality; Raman spectroscopy; sciatic neurectomy; hydroxyapatite; mineral maturity; bone matrix; collagen crosslink
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material

SciFeed

Share & Cite This Article

MDPI and ACS Style

Ishimaru, Y.; Oshima, Y.; Imai, Y.; Iimura, T.; Takanezawa, S.; Hino, K.; Miura, H. Raman Spectroscopic Analysis to Detect Reduced Bone Quality after Sciatic Neurectomy in Mice. Molecules 2018, 23, 3081.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top