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Separation of Volatile Metabolites from the Leaf-Derived Essential Oil of Piper mollicomum Kunth (Piperaceae) by High-Speed Countercurrent Chromatography

1
Laboratório de Produtos Naturais, PN3, FarManguinhos, FIOCRUZ, 21045-900 Rio de Janeiro, Brazil
2
Instituto de Pesquisas de Produtos Naturais (IPPN), Universidade Federal do Rio de Janeiro (UFRJ), 21941-902 Rio de Janeiro, Brazil
3
Centro de Desenvolvimento Tecnológico em Saúde (CDTS/Fiocruz), 21045-360 Rio de Janeiro, Brasil
*
Author to whom correspondence should be addressed.
Molecules 2018, 23(12), 3064; https://doi.org/10.3390/molecules23123064
Received: 30 October 2018 / Revised: 14 November 2018 / Accepted: 20 November 2018 / Published: 23 November 2018
The technique of high-speed countercurrent chromatography was applied to the isolation of compounds in essential oil derived from the leaves of Piper mollicomum species. Plant leaves (200.0 g) were submitted to hydrodistillation in a modified Clevenger apparatus. The resulting crude leaf essential oil was analyzed by gas chromatography with flame ionization detector (GC-FID) and gas chromatography-mass spectrometry (GC-MS) to determine the profile of the components. The purified fractions were composed of monoterpenes and sesquiterpenes such as camphor (85.0 mg at 98.5% purity), (E)-nerolidol (100.0 mg at 92.8% purity), and camphene (150.0 mg at 82.0% purity). A minor component of the essential oil, bornyl acetate (16.2 mg at 91.2% purity) was also isolated in the one-step separation protocol in 2 h. The countercurrent chromatography technique proved to be a fast and efficient method for the separation of volatile metabolites that conserved the solvent while delivering various fractions of high purity. View Full-Text
Keywords: camphor; nerolidol; separation process; CCC; essential oil camphor; nerolidol; separation process; CCC; essential oil
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Marques, A.M.; Peixoto, A.C.C.; Provance, D.W., Jr.; Kaplan, M.A.C. Separation of Volatile Metabolites from the Leaf-Derived Essential Oil of Piper mollicomum Kunth (Piperaceae) by High-Speed Countercurrent Chromatography. Molecules 2018, 23, 3064.

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