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Molecules 2018, 23(11), 2877; https://doi.org/10.3390/molecules23112877

Synthetic Evaluation of Standard and Microwave-Assisted Solid Phase Peptide Synthesis of a Long Chimeric Peptide Derived from Four Plasmodium falciparum Proteins

1
Department of Chemical Synthesis, Fundación Instituto de Inmunología de Colombia (FIDIC), Carrera 50 No. 26-20, Bogotá 111321, Colombia
2
Department of Chemistry, Faculty of Sciences, Universidad Nacional de Colombia, Facultad de Ciencias, Carrera 45 No 26-85, Bogotá 11001, Colombia
3
School of Medicine and Health Science, Universidad del Rosario, Carrera 24 No 63c-69, Bogotá 111221, Colombia
4
Director General, Fundación Instituto de Inmunología de Colombia (FIDIC), Carrera 50 No. 26-20, Bogotá 111321, Colombia
*
Author to whom correspondence should be addressed.
Received: 17 August 2018 / Revised: 25 October 2018 / Accepted: 29 October 2018 / Published: 5 November 2018
(This article belongs to the Section Organic Chemistry)
Full-Text   |   PDF [864 KB, uploaded 5 November 2018]   |  

Abstract

An 82-residue-long chimeric peptide was synthesised by solid phase peptide synthesis (SPPS), following the Fmoc protocol. Microwave (MW) radiation-assisted synthesis was compared to standard synthesis using low loading (0.20 mmol/g) of polyethylene glycol (PEG) resin. Similar synthetic difficulties were found when the chimeric peptide was obtained via these two reaction conditions, indicating that such difficulties were inherent to the sequence and could not be resolved using MW; by contrast, the number of coupling cycles and total reaction time became reduced whilst crude yield and percentage recovery after purification were higher for MW radiation-assisted synthesis. View Full-Text
Keywords: solid phase peptide synthesis; chimeric peptide; coupling; microwave radiation; Fmoc protocol solid phase peptide synthesis; chimeric peptide; coupling; microwave radiation; Fmoc protocol
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Varela, Y.F.; Vanegas Murcia, M.; Patarroyo, M.E. Synthetic Evaluation of Standard and Microwave-Assisted Solid Phase Peptide Synthesis of a Long Chimeric Peptide Derived from Four Plasmodium falciparum Proteins. Molecules 2018, 23, 2877.

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