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Molecules 2018, 23(11), 2786; https://doi.org/10.3390/molecules23112786

Shape Effects of Peptide Amphiphile Micelles for Targeting Monocytes

1,†
,
1,†
,
2,3
and
1,4,5,6,7,*
1
Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USA
2
Institute for Molecular Engineering, University of Chicago, 5747 South Ellis Avenue, Chicago, IL 60637, USA
3
Current Affiliation: Medical Scientist Training Program, David Geffen School of Medicine, University of California, Los Angeles, CA 90096, USA
4
Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, Los Angeles, CA 90089, USA
5
Division of Nephrology and Hypertension, Department of Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
6
Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90033, USA
7
Department of Stem Cell Biology and Regenerative Medicine, University of Southern California, Los Angeles, CA 90033, USA
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: He Dong
Received: 24 September 2018 / Revised: 23 October 2018 / Accepted: 25 October 2018 / Published: 27 October 2018
(This article belongs to the Special Issue Recent Advances in Self-Assembled Peptides)
Full-Text   |   PDF [4377 KB, uploaded 27 October 2018]   |  

Abstract

Peptide amphiphile micelles (PAMs) are a nanoparticle platform that have gained popularity for their targeting versatility in a wide range of disease models. An important aspect of micelle design is considering the type of hydrophobic moiety used to synthesize the PAM, which can act as a contributing factor regarding their morphology and targeting capabilities. To delineate and compare the characteristics of spherical and cylindrical micelles, we incorporated the monocyte-targeting chemokine, monocyte chemoattractant protein-1 (MCP-1), into our micelles (MCP-1 PAMs). We report that both shapes of nanoparticles were biocompatible with monocytes and enhanced the secondary structure of the MCP-1 peptide, thereby improving the ability of the micelles to mimic the native MCP-1 protein structure. As a result, both shapes of MCP-1 PAMs effectively targeted monocytes in an in vitro binding assay with murine monocytes. Interestingly, cylindrical PAMs showed a greater ability to attract monocytes compared to spherical PAMs in a chemotaxis assay. However, the surface area, the multivalent display of peptides, and the zeta potential of PAMs may also influence their biomimetic properties. Herein, we introduce variations in the methods of PAM synthesis and discuss the differences in PAM characteristics that can impact the recruitment of monocytes, a process associated with disease and cancer progression. View Full-Text
Keywords: monocytes; peptide amphiphile micelles; monocyte targeting; nanoparticle shape monocytes; peptide amphiphile micelles; monocyte targeting; nanoparticle shape
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Joo, J.; Poon, C.; Yoo, S.P.; Chung, E.J. Shape Effects of Peptide Amphiphile Micelles for Targeting Monocytes. Molecules 2018, 23, 2786.

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