Development of Validated Methods and Quantification of Curcuminoids and Curcumin Metabolites and Their Pharmacokinetic Study of Oral Administration of Complete Natural Turmeric Formulation (Cureit™) in Human Plasma via UPLC/ESI-Q-TOF-MS Spectrometry
by
Shintu Jude 1, Augustine Amalraj 1
, Ajaikumar B. Kunnumakkara 2, Chandradhara Divya 3, Bernd-Michael Löffler 4 and Sreeraj Gopi 1,*
Shintu Jude 1, Augustine Amalraj 1, Ajaikumar B. Kunnumakkara 2, Chandradhara Divya 3, Bernd-Michael Löffler 4 and Sreeraj Gopi 1,*
1
R&D Centre, Aurea Biolabs (P) Ltd., Kolenchery, Cochin-Kerala 682311, India
2
Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam-781039, India
3
#2/5, Dahlia Building, 3rd Floor, 80 Feet Road, RMV 2nd Stage, Bengaluru, Karnataka 560094, India
4
Institute of Mitochondrial Medicine, Pfalzburger Str. 43/44, 10717 Berlin, Germany
*
Author to whom correspondence should be addressed.
Molecules 2018, 23(10), 2415; https://doi.org/10.3390/molecules23102415
Received: 27 August 2018 / Revised: 14 September 2018 / Accepted: 15 September 2018 / Published: 20 September 2018
Specific and sensitive ultra-high performance liquid chromatography-quadrupole time of flight-mass spectroscopy (UPLC-QTOF-MS) methods have been developed for the determination of curcuminoids and curcumin metabolites in human blood plasma. The UPLC-QTOF-MS method used a binary solvent delivery system and the chromatographic separation was performed on a C-18 (2.1 × 50 mm; 1.7 µm) column. Mass spectra were obtained on a Waters Xevo G2S Q-TOF mass spectrometer. The developed methods to characterize the pharmacokinetics of curcuminoids and curcumin metabolites in human blood plasma after an oral administration of bioavailable curcumin—Cureit™—were validated. It was found that the complete turmeric matrix enhances the concentration of tetrahydrocurcumin (THC), hexahydrocurcumin (HHC), octahydrocurcumin (OHC), curcumin-O-glucuronide (COG) and curcumin-O-sulfate (COS) in the blood plasma once the product is administrated.
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Keywords:
curcuminoids; curcumin metabolites; UPLC/ESI-Q-TOF-MS spectrometry; pharmacokinetic; bioavailable curcumin (Cureit™)
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MDPI and ACS Style
Jude, S.; Amalraj, A.; Kunnumakkara, A.B.; Divya, C.; Löffler, B.-M.; Gopi, S. Development of Validated Methods and Quantification of Curcuminoids and Curcumin Metabolites and Their Pharmacokinetic Study of Oral Administration of Complete Natural Turmeric Formulation (Cureit™) in Human Plasma via UPLC/ESI-Q-TOF-MS Spectrometry. Molecules 2018, 23, 2415.
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