Development, Physicochemical Characterization and In Vitro Anti-Inflammatory Activity of Solid Dispersions of α,β Amyrin Isolated from Protium Oilresin
Abstract
:1. Introduction
2. Results and Discussion
2.1. Physicochemical Characterization
2.1.1. Fourier Transform Infrared—FTIR
2.1.2. Scanning Electronic Microcopy
2.1.3. Powder X-ray Diffraction
2.1.4. Thermogravimetric Analysis
2.1.5. Differential Scanning Calorimetry
2.2. In Vitro Anti-Inflammatory Activity
2.2.1. Quantification of Nitric Oxide in LPS-Stimulated Macrophages
2.2.2. Cell Viability Assays
3. Materials and Methods
3.1. Material
3.2. Preparation of SDs
3.2.1. SD Obtained by PM
3.2.2. SD Obtained by KND
3.2.3. SD Obtained by RE
3.3. Physicochemical Characterization
3.3.1. Fourier Transform Infrared
3.3.2. Scanning Electronic Microscopy
3.3.3. Powder X-ray Diffraction
3.3.4. Thermogravimetry
3.3.5. Differential Scanning Calorimetry
3.4. In Vitro Anti-Inflammatory Study
3.4.1. Quantification of Nitric Oxide (NO)
3.4.2. Cell viability Assay
3.5. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the compounds α,β amyrin. are available from the authors. |
Sample | Concentration (μg/mL) | NO− Concentration | % Inhibition |
---|---|---|---|
LPS | 1 | 45.75 ± 0.855 | 0 |
ABAM | 20 | 25.37 ± 1.237 | 44.55 |
PM-HPMC | 20 | 17.99 ± 2.037 | 60.68 |
PM-PEG | 20 | 16.70 ± 0.200 | 63.50 |
PM-PVP | 20 | 18.81 ± 1.114 | 58.88 |
KND-HPMC | 20 | 20.06 ± 1.195 | 56.15 |
KND-PEG | 20 | 21.41 ± 1.437 | 53.20 |
KND-PVP | 20 | 17.16 ± 0.830 | 62.49 |
RE-HPMC | 20 | 26.53 ± 1.425 | 42.09 |
RE-PEG | 20 | 28.20 ± 0.762 | 38.35 |
RE-PVP | 20 | 22.26 ± 2.306 | 51.13 |
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Da Silva Júnior, W.F.; Pinheiro, J.G.d.O.; De Menezes, D.L.B.; E Silva, N.E.d.S.; De Almeida, P.D.O.; Lima, E.S.; Da Veiga Júnior, V.F.; De Azevedo, E.P.; De Lima, Á.A.N. Development, Physicochemical Characterization and In Vitro Anti-Inflammatory Activity of Solid Dispersions of α,β Amyrin Isolated from Protium Oilresin. Molecules 2017, 22, 1512. https://doi.org/10.3390/molecules22091512
Da Silva Júnior WF, Pinheiro JGdO, De Menezes DLB, E Silva NEdS, De Almeida PDO, Lima ES, Da Veiga Júnior VF, De Azevedo EP, De Lima ÁAN. Development, Physicochemical Characterization and In Vitro Anti-Inflammatory Activity of Solid Dispersions of α,β Amyrin Isolated from Protium Oilresin. Molecules. 2017; 22(9):1512. https://doi.org/10.3390/molecules22091512
Chicago/Turabian StyleDa Silva Júnior, Walter Ferreira, Jonas Gabriel de Oliveira Pinheiro, Danielle Lima Bezerra De Menezes, Natan Emanuell de Sobral E Silva, Patrícia Danielle Oliveira De Almeida, Emerson Silva Lima, Valdir Florêncio Da Veiga Júnior, Eduardo Pereira De Azevedo, and Ádley Antonini Neves De Lima. 2017. "Development, Physicochemical Characterization and In Vitro Anti-Inflammatory Activity of Solid Dispersions of α,β Amyrin Isolated from Protium Oilresin" Molecules 22, no. 9: 1512. https://doi.org/10.3390/molecules22091512