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Molecules 2017, 22(7), 1083;

The C5 Variant of the Butyrylcholinesterase Tetramer Includes a Noncovalently Bound 60 kDa Lamellipodin Fragment

Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198-5950, USA
Hôpital d’Instruction des Armées Bégin-Département des Laboratoires, 69, Avenue de Paris, 94 163 Saint Mandé, France
Neuropharmacology Laboratory, Kazan Federal University, 420000 Kazan, Russia
CNRS Université Paris Descartes Cognition Action Group UMR 8257, 75006 Paris, France
Author to whom correspondence should be addressed.
Received: 3 June 2017 / Revised: 27 June 2017 / Accepted: 27 June 2017 / Published: 29 June 2017
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Humans with the C5 genetic variant of butyrylcholinesterase (BChE) have 30–200% higher plasma BChE activity, low body weight, and shorter duration of action of the muscle relaxant succinylcholine. The C5 variant has an extra, slow-moving band of BChE activity on native polyacrylamide gel electrophoresis. This band is about 60 kDa larger than wild-type BChE. Umbilical cord BChE in 100% of newborn babies has a C5-like band. Our goal was to identify the unknown, 60 kDa protein in C5. Both wild-type and C5 BChE are under the genetic control of two independent loci, the BCHE gene on Chr 3q26.1 and the RAPH1 (lamellipodin) gene on Chr 2q33. Wild-type BChE tetramers are assembled around a 3 kDa polyproline peptide from lamellipodin. Western blot of boiled C5 and cord BChE showed a positive response with an antibody to the C-terminus of lamellipodin. The C-terminal exon of lamellipodin is about 60 kDa including an N-terminal polyproline. We propose that the unknown protein in C5 and cord BChE is encoded by the last exon of the RAPH1 gene. In 90% of the population, the 60 kDa fragment is shortened to 3 kDa during maturation to adulthood, leaving only 10% of adults with C5 BChE. View Full-Text
Keywords: lamellipodin; RAPH1; butyrylcholinesterase; tetramer; C5 phenotype lamellipodin; RAPH1; butyrylcholinesterase; tetramer; C5 phenotype

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Schopfer, L.M.; Delacour, H.; Masson, P.; Leroy, J.; Krejci, E.; Lockridge, O. The C5 Variant of the Butyrylcholinesterase Tetramer Includes a Noncovalently Bound 60 kDa Lamellipodin Fragment. Molecules 2017, 22, 1083.

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