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Molecules 2017, 22(5), 748; doi:10.3390/molecules22050748

Article
Anti-Onchocerca and Anti-Caenorhabditis Activity of a Hydro-Alcoholic Extract from the Fruits of Acacia nilotica and Some Proanthocyanidin Derivatives
1
Department of Biological Sciences, Faculty of Science, University of Ngaoundere, P.O. Box 454, Ngaoundere, Cameroon
2
Institute of Pharmaceutical Biology and Phytochemistry (IPBP), University of Münster, PharmaCampus Correnstrasse 48, D-48149 Münster, Germany
3
Institute for Zoophysiology, University of Münster, Schlossplatz 8, D-48143 Münster, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 7 April 2017 / Accepted: 3 May 2017 / Published: 6 May 2017

Abstract

:
Acacia nilotica fruits with high tannin content are used in the northern parts of Cameroon as anti-filarial remedies by traditional healers. In this study, the hydro-alcoholic fruit extract (crude extract (CE)) and, one of the main constituents in its most active fractions, (+)-catechin-3-O-gallate (CG), as well as four related proanthocyanidins, (−)-epicatechin-3-O-gallate (ECG), (+)-gallocatechin (GC), (−)-epigallocatechin (EGC) and (−)-epigallocatechin-3-O-gallate (EGCG), were assessed for their potential in vitro anthelmintic properties against the free-living model organism Caenorhabditis elegans and against the cattle filarial parasite Onchocerca ochengi. Worms were incubated in the presence of different concentrations of fruit extract, fractions and pure compounds. The effects on mortality were monitored after 48 h. The plant extract and all of the pure tested compounds were active against O. ochengi (LC50 ranging from 1.2 to 11.5 µg/mL on males) and C. elegans (LC50 ranging from 33.8 to 350 µg/mL on wild type). While high LC50 were required for the effects of the compounds on C. elegans, very low LC50 were required against O. ochengi. Importantly, tests for acute oral toxicity (lowest dose: 10 mg/kg) in Wistar rats demonstrated that crude extract and pure compounds were non-toxic and safe to use. Additionally, the results of cytotoxicity tests with the Caco-2 cell line (CC50 ranging from 47.1 to 93.2 µg/mL) confirmed the absence of significant toxicity of the crude extract and pure compounds. These results are in good accordance with the use of A. nilotica against nematode infections by traditional healers, herdsmen and pastoralists in Cameroon.
Keywords:
onchocerciasis; Acacia nilotica; Caenorhabditis elegans; Onchocerca ochengi; proanthocyanidins

1. Introduction

Onchocerciasis (river blindness) is a tropical insect-borne disease caused by the nematode Onchocerca volvulus and transmitted through the bite of infected black flies (Simulium spp.). It is classified by the World Health Organization (WHO) as one of the tropical neglected diseases (NTDs). It is widespread in Sub-Saharan Africa and endemic in 19 African countries with some foci in Yemen and parts of Latin America. The disease is mainly characterized by skin irritations and eye lesions and can ultimately lead to blindness [1]. It has been estimated that 36 million people are infected [2], and 86 million people live in high risk areas of the African Programme for Onchocerciasis Control (APOC) countries. Onchocerciasis is responsible for about 270,000 cases of blindness and 500,000 cases of visual impairment [1]. Furthermore, people with this disease lose their productivity, thereby affecting the economy of the endemic countries.
Control strategies currently in use rely on mass administration of ivermectin (Mectizan). While this drug has marked activity against microfilariae, it is much less effective against adult worms. In Cameroon, ivermectin was reported to induce some severe inflammatory reactions in people co-infected with loasis [3]. Furthermore, the development of resistance to ivermectin was observed in some communities in Ghana and Sudan [4,5,6]. Treatment with antibiotics, such as tetracycline or doxycycline, targeting the endobacteria Wolbachia, has also been reported [7,8]. However, its use is restricted to only some nematodes, since others such as Loa loa do not have Wolbachia [9]. Furthermore, continuous use of antibiotics is known to lead to the emergence of resistance [9]. Since vaccines and safe macrofilaricidal treatment against Onchocerca volvulus are still lacking, there is an urgent need to discover novel drugs. Natural sources, such as plants, represent a major opportunity to discover new lead molecules.
In our study, we investigated the bovine parasite Onchocerca ochengi that is also transmitted by Simulium damnosum and considered to be the closest relative of O. volvulus. It is currently the best model available for performing research in chemotherapy and immunology of onchocerciasis, and it is demonstrated that drugs used against the bovine parasite also affect the human parasite [10].
Research over many years has demonstrated the medicinal usefulness of many plants to combat parasitic nematodes. An online search on medicinal plants used against onchocerciasis found nine publications since 2002, where a total of 17 plant species, belonging to 10 different families, have been studied [11]. In these studies, only two pure compounds were isolated and tested on Onchocerca gutturosa [12]. Commercially available pure compounds, such as gallic acid, gentisic acid and ellagic acid, have been tested on O. ochengi and against the non-pathogenic nematode model organism Caenorhabditis elegans [13]. The majority of studies have shown in vitro nematocidal activities of plant extracts (Homalium africana, Margaritaria discoidea, Anogeissus leiocarpus, Khaya senegalensis, Euphorbia hirta, Annona senegalensis, Hagenia abyssinica, Acer rubrum, Rosa multiflora, Quercus alba) on C. elegans and O. ochengi [14,15,16,17,18,19]. Fractions from Craterispermum laurinum and Morinda lucida have shown activities with LC50 concentrations ranging from 7.8 µg/mL to 46.8 µg/mL on O. ochengi. The lowest LC50 values of secondary metabolites (AMJI and linoleic acid) from Cyperus articulates recorded were 15.7 µg/mL and 55.7 µM on the same worm [20,21,22]. Up to present, no pure compound has shown activity with an LC50 lower than that of ivermectin (1.7 µM) on O. ochengi.
Acacia nilotica is claimed by local traditional healers in Cameroon to be an effective anthelmintic [23]. Acacia nilotica (Linn) Willd ex Delile, also known under the synonyms Acacia arabica (Lam) var. nilotica (L.) Benth., Acacia scorpioides (L.) var. nilotica (L.) A. Chev., Mimosa nilotica Linn., is widely distributed in different regions of the world (Australia, Asia, Africa and America) [23,24]. A. nilotica is found in the northern part of Cameroon, and it is known in Cameroonian folk medicine by the common names of “Galbiki” or “Gavdi” in Foulfoudé and “Acacia du Nil” in French [23]. Different parts of the tree (gum, leaves, root, fruits, seeds, stem bark, branches and wood) are traditionally used in Africa for treating colds, bronchitis, pneumonia, ophthalmia, diarrhea and hemorrhage. Furthermore, A. nilotica was shown to have anti-bacterial, anthelmintic, antiplasmodial, anti-mutagenic, antipyretic, antioxidant and anti-hypertensive activities [25].
Acacia nilotica contains a variety of bioactive components, such as gallic acid, ellagic acid, isoquercetin, leucocyanidin, kaempferol-7-diglucoside, naringenin-7-O-β-d-(6′-O-galloyl) glucopyranoside, rutin, apigenin-6,8-bis-C-glucopyranoside, m-catechol and their derivatives, as well as galloylated derivatives of (+)-catechin and (+)-gallocatechin [26,27,28]. A literature survey on A. nilotica revealed anthelmintic effects against the intestinal helminth Haemonchus contortus [29,30].
This paper reports, for the first time, the activity of the hydro-alcoholic crude extract of Acacia nilotica, some of its polyphenol-rich fractions and one of its major constituents, catechin-3-O-gallate, against the cattle parasite Onchocerca ochengi and mutant anthelmintic-resistant strains of Caenorhabditis elegans. Furthermore, some structurally-related proanthocyanidins, namely (+)-gallocatechin, (−)-epicatechin-3-O-gallate, (−)-epigallocatechin and (−)-epigallocatechin-3-O-gallate, were also included in the tests and found active with LC50 concentrations in the low micromolar range. The tested compounds also demonstrated nematocidal activity against Caenorhabditis elegans wild type and various drug-resistant strains, but only at higher concentrations. Their cytotoxicity, as well as acute oral toxicity were also assessed.

2. Results

2.1. Dereplication of the Active Fractions and Isolation of Catechin-3-O-Gallate as the Major Constituent

The active fractions were analyzed by UHPLC/+ESI MSMS in order to detect and characterize their main constituents (see Figure 1 and Figure 2). They were found to contain the proanthocyanidins gallocatechin and catechin along with galloylated derivatives as could be deduced from the characteristic molecular ions and fragment mass signals obtained at high resolution that allowed unambiguous determination of the elemental formulas (see Figure 2). Besides the peaks of gallocatechin (1) and catechin (2), the fractions contained various galloyl esters of these proanthocyanidins, namely with galloylation in the A-, B and C-ring as previously reported from this plant [26], which could be distinguished by careful inspection of their mass spectral fragmentation. Thus, Peaks 3 to 5 all represent isomers of elemental formula C22H18O11 corresponding to mono-galloylated gallocatechin. Peaks 6 and 7 corresponded to monogalloylated catechin derivatives (elemental formula C22H18O10), whereas Peak 8 could be assigned a catechin digallate (C29H22O14). As a major component, catechin-3-O-gallate (CG; Peak 7 in Figure 1 and Figure 2) was isolated by preparative. HPLC (retention time = 20 min; UV: λmax = 202 nm) from Fraction 8 and identified by NMR spectroscopy. Its data were identical with those published on this compound isolated from Camellia sinensis and Piptadenia pervillei [31,32]. CG was subsequently investigated for anti-nematode activity as the pure compound together with its stereoisomer epicatechin-3-O-gallate (ECG) and three further structurally-related compounds (gallocatechin (GC); epigallocatechin (EGC) and epigallocatechin-3-O-gallate (EGCG). The structures of the tested compounds are shown in Figure 3.

2.2. Nematocidal Activity against C. elegans

During the screening of plant extract for anthelminthic activity, the crude hydro-alcoholic extract of Acacia nilotica fruits (crude extract (CE)) showed activity against the free-living nematode C. elegans and the cattle parasite O. ochengi (see Table 1). The extract was fractionated by column chromatography on silica, and the 16 fractions obtained were tested against C. elegans. Of the 16 fractions, the first six display required higher concentrations than 1000 µg/mL; the most active were Fractions 8 to 10 with LC50 of 73.8 ± 0.2; 70.1 ± 0.3; 51.6 ± 0.9 µg/mL, respectively (see Supplementary Materials Table S1). These fractions were therefore selected for analysis of their constituents of which the most abundant was catechin-3-O-gallate.
The pure catechin-3-O-gallate, as well as the other related proanthocyanidins were tested for nematocidal activity using young adults of C. elegans. Levamisole, ivermectin and albendazole were used as the positive reference. No death was observed during the experimental period of 48 h in the negative controls. Table 1 shows the LC50 results of the tested compounds. Compared to levamisole (LC50 values of 7.2 µM), the crude extract exhibited a higher LC50 of 350 μg/mL after 48 h on C. elegans N2 Bristol WT. The crude extract therefore causes a much lower mortality than the positive control. Table 1 shows that the five proanthocyanidins tested as pure compounds exhibit nematocidal activity at LC50 below that of the crude extract at the same exposure period (48 h).
Table 1 shows that the crude extract is most potent on the WT strain compared to the levamisole-, ivermectin- and albendazole-resistant strains. All tested drug exhibited the mortality effect on all strains of C. elegans used at different LC50 values (Table 1).
Among the tested samples, WT is moderately sensitive to ECG (LC50 2.4 µM) and EGCG (LC50 108.6 µM) compared to EGC, GC and CG (LC50 204.7; 242.4; 204.8 µM, respectively) (see Figure 3 for the structures of the compounds). Additionally, the ivermectin-resistant strains VC722 (LC50 61.4 and 95.4 µM respectively) and DA1316 (LC50 61.9 and 89.1 µM respectively) are highly sensitive to ECG and moderately sensitive to EGCG. A similar effect is observed with the albendazole-resistant strain CB3474 (LC50 44.2 and 77.9 µM respectively) (Table 1).
The levamisole-resistant strain of C. elegans CB211 (LC50 70.2 µM) is highly sensitive, while ZZ16 (LC50 214.2 µM) is moderately sensitive to GC. The four other compounds are less sensitive on these two strains as shown in Table 1. Additionally, the levamisole-resistant strain CB211 is also highly sensitive to EGCG (LC50 7.4 µM). Albendazole displays the highest LC50 (26.4 µM) compared to the two other reference drugs, while ivermectin exhibiting the lowest LC50 (1.3 µM). In general lethal concentrations (LC) are higher on C. elegans compared to O. ochengi. The WT is not highly active to the compounds. The isolated compound exhibited lethality at least three-fold compared to the CE (Crude extract).

2.3. Nematocidal Activity against Onchocerca ochengi

The compounds were also assessed for their toxicity to microfilariae (Mf), male and female O. ochengi. Levamisole, ivermectin and albendazole were used as positive references. As already mentioned above, the CE displayed a remarkable activity against all three forms of the parasite with LC50 values between 1.0 and 12.0 µg/mL.
Table 1 shows that EGCG (LC50 2.6, 2.3 and 2.8 µM against male, female and Microfilariae) and ECG (LC50 4.7, 4.7 and 2.2 µM, respectively) exhibit the highest mortality of the tested compounds (see Figure 3 for the structures of the compounds). The activity level of EGCG was in the same range as that of the positive control, levamisole (5.1, 10.2 and 5.1 µM, respectively). The other tested pure proanthocyanidins displayed a somewhat weaker, but still very significant, activity.
It is therefore clear that the proanthocyanidins present in the active fractions of A. nilotica are the constituents mainly responsible for the crude extract’s nematocidal activity.

2.4. Cytotoxicity against Caco-2 Cells

The results in terms of CC50 values shown in Table 2 demonstrate that the viability of Caco-2 cells is inhibited by the compounds. There is no difference between the CC50 values for GC and for those of ECG and CG because the value for GC is within the ± SD of ECG and within the ± SD of CG. The crude extract is less toxic than the conventional anthelmintics tested (Table 2). The order of CC50 toxicity is therefore CE > CG = ECG = GC > EGCG > EGC. Comparison of the selectivity indices (SI = CC50/LC50) calculated for C. elegans and O. ochengi (Table 2) again shows that C. elegans is much less sensitive to the tested chemicals than the mammalian control cells, whereas O. ochengi is significantly more sensitive. Generally, the SI of the crude extract and almost all compounds indicated good selective anthelminthic activity on parasites (O. ochengi). This shows that some selectivity of the compounds under study exists. Hence, it will justify their more detailed investigation against this parasite.

2.5. Acute Mammalian Toxicity

An acute toxicity study was performed to evaluate the safe administrable doses of A. nilotica (L.) fruit extract and isolated proanthocyanidins on albino Wistar rats for 14 days. In vivo studies revealed that no abnormal behavior and no mortality during the treatment and observation periods were observed in animals treated at the doses employed. The results of the acute oral toxicity tests of the five compounds CG, ECG, GC, EGC and EGCG, as well as the hydro-alcoholic crude extract of A. nilotica using a rat model are presented in Table S2. The low doses of 10, 100 and 1000 mg/kg of each drug used in Phase I and administered orally to a group of three rats led to no mortality. As no mortality was observed in Phase I, doses were increased to 1500, 3000 and 5000 mg/kg. Even at these doses, no mortality was recorded. Adverse reactions like increased motor activity, blinking eyes, tremors, convulsion, lacrimation, stimulation, muscle weakness, sedation, urination, salivation, lethargy, sleep, arching and rolling and coma up to a dose of 5000 mg/kg were not noticed within 14 days. Our results confirm that the doses tested were harmless for further in vivo investigations via gavage.

3. Discussion

Previous reports state that A. nilotica has anthelmintic activity against Haemonchus contortus and suggest the lipophilic nature of the active principle in bark and leaves [33]. In a separate study, a variety of bioactive components such as gallic acid, ellagic acid, isoquercetin, leucocyanidin and kaempferol have been isolated from A. nilotica [28,34]. Even though some of these phenolic compounds obtained from other sources have been tested against O. ochengi [13], as far as could be ascertained, this is the first report on the anthelminthic activity of CG, isolated from the fruit of A. nilotica, and on its structural congeners, ECG, GC, EGC, EGCG as individual compounds on this bovine parasitic nematode, O. ochengi, and the mutant anthelmintic-resistant C. elegans.
This study was undertaken to assess the anthelmintic efficacy of the crude extract of A. nilotica, as well as its major constituent CG against the bovine filarial nematode O. ochengi and the free-living nematode C. elegans. Some further related proanthocyanidins, ECG, GC, EGC and EGCG, were also included in the study because of their chemical similarity to the A. nilotica constituents. The nematodes under study have widely been used to evaluate the efficacy of several anti-filarial agents [20,21,22,35,36]. In this study, the toxicity of the extract and the pure compounds was investigated against C. elegans WT and five drug-resistant mutant strains (ZZ16, CB211, CB3474, VC722, DA1316). Furthermore, the activity against two different life cycle stages (macrofilariae and microfilariae) of the cattle parasite O. ochengi was assessed. Our results demonstrate the sensitivity of both nematodes to the pure compounds; the parasite being significantly more sensitive than the free-living nematode. Although there are many reports from Asia, Africa, America and Europe on natural plant extracts or plant-derived compounds that have anthelmintic properties, no major commercial product based on these natural compounds has been developed yet for wide use [19,37,38]. A. nilotica has been reported to be a rich source of tannin [26,27,28,29,30]. Condensed and hydrolysable tannins were shown to have anthelmintic potential with various degrees [13,15]. The crude extract tested in this study exhibited LC50 values (449 and 350 μg/mL), at 24 h and 48 h post-treatment, respectively (result at 24 h not shown), less than those reported in the literature on other plants rich in tannin, as well: Quercus alba (0.75 mg/mL), Rhus typhina (0.65 mg/mL) Acer rubrum (1.03 mg/mL) and Rosa multiflora (2.14 mg/mL) against C. elegans at 24 h post-treatment [15].
Our experiments showing an LC50 = 108.6 µM of EGCG on C. elegans wild type are in contrast with in vitro evidence of increased lifespan of C. elegans. In 2009, Zhang et al. reported that EGCG enhances the resistance of C elegans to environmental stress and prolongs its longevity by removing reactive oxygen species (ROS) when administered on C. elegans fed with E. coli OP50. However, in that study, the authors exposed two-day-old adult wild type to E. coli containing the compound on solid medium at the highest concentration of 10 µg/mL; EGCG did not extend the longevity of WT under normal culture conditions, but led to an increase in stress tolerance [39]. In contrast to these authors, we used much higher concentrations of EGCG and incubated worms without OP50 as the source of food. All five pure proanthocyanidins showed higher mortality on WT (LC50: ECG 2.4; EGCG 108.6; EGC 204.7; GC 242.4; CG 204.8 µM) compared to the crude extract (LC50 350 μg/mL). Proanthocyanidins vary in the number of phenolic hydroxyl groups and in their stereochemistry. The difference in the efficacy of the compounds, in this study, may be due to trihydroxylated aromatic groups, i.e., the galloyl ester group and a pyrogalloyl type B-ring. Each of the five tested compounds harbored either a pyrogalloyl type B-ring or a galloyl group, but only EGCG has both groups, which might explain its high toxicity, it required higher LC50 values than ECG. Mukai et al. [40] also reported that EGCG toxicity surpasses mebendazole. It was also shown in the same study that the pyrogalloyl type B-ring of EGC and EGCG enhances the toxicity compared to ECG. Similarly, the galloyl group of ECG and EGCG also has great significance in causing toxicity [40]. In another study, the toxicity of gallotannins and condensed tannins towards C. elegans has been shown to be dependent on the degree of galloylation and polymerization, respectively [41]. The two isomers CG and ECG have different lethality on the worms. The first has the highest LC50 values on WT and parasite, which cannot be explained by the galloyl group. It might be that the stereochemistry of the proanthocyanidin core plays an important role.
Tannins are known to bind to free proteins in the gastrointestinal tract of host animal or glycoprotein on the cuticle of the parasite [42] and cause death. Here, we assess whether the compounds’ mode of action might be similar to those of the three classes of the most used anthelmintics: avermectins, which are glutamate-gated chloride (GluCl) potentiators, imidazothiazoles, which are nicotinic agonists, and benzimidazoles, which are ß-tubulin binding [42]. Our results revealed a varying lethality of the five resistant C. elegans strains to the compounds under study. CB211 and ZZ16 are knockout mutants of the genes lev-1 and lev-9, respectively. The gene lev-1 is expressed in body wall muscle and is important for normal locomotion and regulation of egg-laying [43]; the gene lev-9 is also secreted in muscle cells and is responsible for locomotion and egg-laying. Compared to the WT (LC50 74.3 μg/mL), GC displays mortality of half of the population on the levamisole resistant strains (ZZ16 and CB211) at lower LC50 214.2 and 70.2 µM, respectively. Only mutant CB211 is sensitive to EGCG (LC50 57.4 µM) (Table 1). This suggests that their efficacy is independent of the levamisole resistance and that the mode of action of the compounds differs from that of levamisole. Levamisole belongs to the imidazothiazoles, which are nicotinic receptor agonists [44,45]. However, mutants CB211 and ZZ16 showed a reduced sensitivity towards CG, ECG and EGC (Table 1), suggesting that the effects of these three compounds might be similar to levamisole. CB3474 is a knockout mutant of ben-1. This gene encodes β-tubulin that represents the binding site of albendazole, inhibiting the formation of microtubules [46,47] and resulting in the paralysis of the worms [48]. Albendazole is one of the benzimidazole carbamates [44,45]. Compared to WT, the albendazole-resistant strain (CB3474) is highly sensitive to ECG (LC50 44.2 µM) and EGCG (LC50 77.9 µM), suggesting that the mode of action of these compounds differs from that of albendazole. However, contrary to ECG and EGCG, the mutant CB3474 showed a reduced sensitivity towards GC, CG and EGC (Table 1), suggesting that these three compounds may act similarly to albendazole.
Ivermectin is a drug classified amongst the macrocyclic lactones [45]. It is a GluCl receptor potentiator [49]. It specifically binds to GluCl channels and selectively paralyzes the parasite by increasing muscle and nerve chloride-ion permeability, thereby causing the death of worm [44]. VC722 is a single mutant in which the GluCl subunit glc-2 has been knocked out. Glc-2 represents the binding site of ivermectin in pharyngeal muscle cells [50]. DA1316 is a triple mutant in which genes avr-14, avr-15 and glc-1 are knocked out. Genes avr-14 and glc-1 represent the binding site of ivermectin in the nervous system, while the gene avr-15 plays the same role as glc-2 [50,51]. Compared to WT, the single (VC722) and triple (DA1316) ivermectin-resistant strains are highly sensitive to ECG (LC50 61.4; 61.9 µM, respectively) and moderately sensitive to EGCG (LC50 95.4; 89.1 µM, respectively) (Table 1). This suggests that their efficacy is independent of genes transferring resistance to the strains and may be due to the chemical structures of both molecules, which are almost identical, except for one additional OH on EGCG. Since genes conferring resistance in both mutants are in muscle cells and nervous system, high activities of EGCG and ECG observed on mutants VC722 and DA1316 could be explained by the fact that these two compounds act neither in muscle cells nor in nervous system, but in another tissue. The stereochemistry of the proanthocyanidin core appears to play an important role. Compounds of the epi-series (B-ring and Oxygen at C-3 cis-oriented), such as ECG and EGCG, appear more active than compounds with trans-orientation of these structure elements (CG and GC). Taken the high sensitivity of the ivermectin resistant strains to ECG and EGCG, their mode of action differs from that of ivermectin. Mutants VC722 and DA1316 show only a slightly reduced sensitivity towards GC, CG and EGC (Table 1). This might suggest that these three compounds do not act similarly to ivermectin. The five isolated proanthocyanidins thus appear to have a mode of action different from those of the commonly-used anthelmintic, ivermectin. However, they might act synergically with gallic and ellagic acid in the crude extract since their nematotoxicity has been shown [13]. It has long been known and demonstrated in various studies that tannins and other polyphenolic compounds are protein coagulants, which could result in a broad spectrum worm killing activity [52,53]. Iqbal et al. [54] suggested that condensed tannins may also bind to the cuticle of larvae, which is rich in glycoprotein according to Thompson and Geary [55], and cause death [54].
Results obtained after the exposure of O. ochengi to the five compounds reveal strong mortality. This result is in agreement with a recent study that showed that hydrolysable tannins, such as gallic acid and ellagic acid, have high levels of anthelmintic activity [13]. The present results are in agreement with these findings, since ECG and EGCG are galloylated proanthocyanidins, which contain a gallic acid moiety in their structure. Compared to the high concentrations required for the efficacy of the compounds on C. elegans, considerably lower concentrations are required for efficacy on O. ochengi. This appears to be in agreement with the statement of Geary et al. [35] on the disadvantage of using C. elegans as a model to estimate the intrinsic potency of anthelmintics against parasitic nematodes. The presence of galloylated proanthocyanidins in the most active fractions of A. nilotica extract rationalizes the use of this plant in folk medicine. The high level of activity of the tested compounds of this type against O. ochengi, which is in the same range as that of the positive controls, makes this class of plant phenols very promising candidates for further detailed studies.
The acute toxicity assay carried out with the drugs at a dose of 5000 mg/kg caused neither behavioral changes nor other signs of toxicity or even death in any of the rats tested during the 14-day observation period. Hence, the LD50 could not be determined. The compounds did not lead to any adverse reactions like increased motor activity, blinking eyes, tremors, convulsion, lacrimation, stimulation, muscle weakness, sedation, urination, salivation, lethargy, sleep, arching, rolling or coma up to a dose of 5000 mg/kg. Any test substance showing an LD50 of 1000 mg/kg after oral administration can be considered safe [56]. This result indicates that the compounds under study, when given orally, could be considered relatively safe. Recently, Mohan et al. [56] observed no mortality with an A. nilotica leaf extract on oral administration to rats at doses up to 2000 mg/kg; while Gouta et al. [57] reported in mice LD50 = 7393.4 mg/kg of the ethyl acetate fruit fraction. With respect to in vitro cytotoxicity, each drug tested behaved distinctly in the cell line. The distinct effects may be due to either their diversity or diverse mechanisms associated with each of the compounds. The cytotoxicity tests [58] confirm the reported result of procyanidin reducing cell viability for 15% at 127 µg/mL in Caco-2 cells. The selectivity index of almost all proanthocyanidins indicated good selective anthelminthic activity on the nematode parasite O. ochengi (as indicated in Table 2) given the fact that they are of cancerous origin even though they are widely use in transport studies. However, these selectivity indexes may need to be improved for further studies as much as possible according to Huggins et al. [59].

4. Materials and Methods

4.1. General Instrumentation for Phytochemical Analysis and Purification

UHPLC/+ESI-QqTOF MS analyses were carried out on a Bruker MicrOTOF-QII mass spectrometer coupled to a Dionex Ultimate 3000 RS UHPLC equipped with a Dionex Acclaim RSLC 120, C18 column (2.1 × 100 mm, 2.2 µm) with a binary gradient (A: water with 0.1% formic acid; B: acetonitrile with 0.1% formic acid) at 0.8 mL/min: 0 to 9.5 min: linear from 5% B to 100% B; 9.5 to 12.5 min: isocratic 100% B; 12.5 to 12.6 min: linear from 100% B to 5% B; 12.6 to 15 min: isocratic 5% B. The injection volume was 2 µL. UV/DAD detection was performed over a wavelength range of 200 to 400 nm. Mass spectrometric detection was performed with a Bruker Daltonics MicrOTOF-QII time-of-flight mass spectrometer equipped with an Apollo electrospray ionization source in positive mode at 5 Hz over a mass range of m/z 50 to 1000 using the following instrument settings: nebulizer gas nitrogen, 5 bar; dry gas nitrogen, 9 L/min, 220 °C; capillary voltage 4500 V; end plate offset −500 V; transfer time 70 µs; collision gas nitrogen; collision energy and collision RF settings were combined to each single spectrum of 1000 summations as follows: 250 summations with 20% base collision energy and 130 Vpp + 250 summations with 100% base collision energy and 500 Vpp + 250 summations with 20% base collision energy and 130 Vpp + 250 summations with 100% base collision energy and 500 Vpp. Base collision energy was 50 eV for precursor ions with a m/z less than 500 and then linearly interpolated against m/z up to a maximum of 70 eV for precursor ions with an m/z of up to 1000. Internal dataset calibration (HPC mode) was performed for each analysis using the mass spectrum of a 10 mM solution of sodium formate in 50% isopropanol that was infused during LC re-equilibration using a divert valve equipped with a 20-µL sample loop. Sample concentrations: the crude extract was injected at a concentration of 5 mg/mL, fractions at 0.5 mg/mL.
Analytical HPLC UV-DAD was performed with a Jasco (Groß-Umstadt, Germany) HPLC system with autosampler AS-2055 plus, ternary pump PU-2055 plus, degasser DG-2080-54, column oven Jetstream plus, set at 25 °C, UV-DAD-detector: MD-2010 plus, Software “Chrompass” for instrument control and data processing. A Macherey & Nagel (Düren, Germany) Nucleodur RP-18 column (125 × 4.6 mm, 5 µm, endcapped) with a column guard was used. Water (aqua Millipore) (A) with 0.1% TFA: acetonitrile (B) with 0.1% TFA gradient was used at a flow rate of 1 mL/min; gradient: 0 min: 95% (A), 50 min: 30% (A), 52 min: 20% (A), 55 min: 0% (A); 60 min: 0% (A); re-equilibration: 10 min; injection volume: 10 µL. Samples were dissolved in MeOH (HPLC-grade) with a concentration of 1 mg/mL.
Preparative HPLC separations were performed on a Jasco (Groß-Umstadt, Germany) prep. HPLC system (pump: PU-2087 plus; diode array detector MD 2018 plus; column thermostat CO 2060 plus; autosampler AS 2055 plus; LC Net II ADC Chromatography Data Solutions; sample injection loop: 2000 µL) on a Reprosil 100 C-18 column (5 µm, 250 × 20 mm) column with a binary gradient: water with 0.1% trifluoroacetic acid (A); acetonitrile with 0.1% trifluoroacetic acid (B) at 15 mL/min with: 0 to 5 min: linear from 5% B to 25% B; 5 to 10 min: linear 25% B to 30% B; 10 min to 35 min: linear from 30% B to 70% B; 35 min to 38 min: linear 70% B to 100% B; 30 min to 35 min: isocratic 100% B; injection volume: 283 µL. Samples were dissolved in MeOH (HPLC-grade) with a concentration of 10 mg/mL.
NMR spectra were acquired at 303 K in methanol-d4 on an Agilent DD2 600 spectrometer (Santa Clara, CA, USA), operating at 14.1 Tesla, observing 1H and 13C at 600 and 150 MHz.

4.2. Chemicals and Plant Material

Acacia nilotica fruits, used in this study, were harvested in January 2012 from the Far-North regions of Cameroon (GPS: 10°61′24.1″ N and 14°36′08.4″ E at an altitude of 392 m above sea level). Preliminary identification was made by botanists of the Department of Biological Sciences, University of Ngaoundere, Cameroon. A voucher specimen was deposited in the National Herbarium of Yaounde (Voucher Number 8582HNC).
Epicatechin-3-O-gallate was purchased from Sigma (Deisenhofen, Germany). Samples of (+)-gallocatechin; (−)-epigallocatechin and (−)-epigallocatechin-3-O-gallate, were kindly provided by F. Petereit, IPBP (Institute of Pharmaceutical Biology and Phytochemistry) University of Münster; gallocatechin was originally isolated from Cistus albidus [60]; epigallocatechin and epigallocatechin-3-O-gallate were isolated from Cistus salvifolius [61]. If not stated otherwise, all other chemicals were purchased from Sigma (Deisenhofen, Germany).

4.3. Extraction, Bioassay-Guided Fractionation and Isolation of Catechin-3-O-Gallate

4.3.1. Hydro-Alcoholic Extraction from A. nilotica Fruits

The collected plant material was air-dried for three weeks at room temperature (25 to 28 °C), powdered, then sieved (size: 1 mm) before extraction. The air-dried and powdered fruits (500 g) were extracted with 5 L of ethanol:water (60:40 v/v) for 48 h at room temperature. The extract was filtered using Whatman No. 1 filter paper. The extraction was repeated three times, and the ethanol was removed using a rotary evaporator under reduced pressure at 40 °C. The aqueous solution was frozen and lyophilized to obtain a hydro-alcoholic extract. In this way, 142.9 g of crude hydro-alcoholic extract were obtained (yield: 35.65% w/w). Five milligrams were submitted to biological tests.

4.3.2. Fractionation of Hydro-Alcoholic Extract of A. nilotica Fruits

Four (4) grams of the hydro-alcoholic extract of A. nilotica were chromatographed over silica gel 60 (300 g) using a glass column (90 cm high and 5 cm in diameter). The column was packed with a silica gel slurry prepared in hexane: ethyl acetate (EtOAc) (70:30). The sample was mixed with silica, dried and carefully introduced on top of the column. The elution was initiated with hexane: ethyl acetate (70:30) followed by mixtures of increasing EtOAc concentration 50:50, 40:60, 20:80 up to 10:90 (250 mL were used for elution in each step). The column was finally washed with 200 mL of methanol. The sub-fractions were pooled together after TLC control (20 cm × 20 cm silica gel 60 F254, 1 mm, elution with hexane:ethyl–acetate (1:1)) and 16 fractions were obtained: Fr. 1 (8.4 mg), Fr. 2 (3.0 mg), Fr. 3 (20.2 mg), Fr. 4 (28.7 mg), Fr. 5 (49.5 mg), Fr. 6 (35.7 mg), Fr. 7 (70.2 mg), Fr. 8 (70.9 mg), Fr. 9 (62.9 mg), Fr. 10 (48 mg), Fr. 11 (51.2 mg), Fr. 12 (244.5 mg), Fr. 13 (182.8 mg), Fr. 14 (58.8 mg), Fr. 15 (2.7 mg) and Fr. 16 (51.8 mg). 5 mg of each fraction were tested for anthelmintic activity against C. elegans. The most active fractions (8, 9, 10) were subjected to ultra-high performance liquid chromatography/mass spectrometry (UHPLC/+ESI-QqTOF MSMS) and high performance liquid chromatography (HPLC) to obtain chromatographic profiles.
The high resolution +ESI MS and MSMS data of the active fractions indicated the presence of catechin and gallocatechin derivatives by the characteristic molecular ions and fragmentation (see Figure 1 and Figure 2). From Fraction 8, the main constituent catechin-3-O-gallate was isolated by prep HPLC. The isolated compound was identified unambiguously by 1D and 2D-NMR spectroscopic measurements. All data were in agreement with the literature [31,32].

4.4. Nematocidal Activity of the Pure Compounds

4.4.1. Caenorhabditis elegans Strains and Monoxenic and Axenic Culture Conditions

The following C. elegans strains were used: N2 Bristol, referred to as wild type (WT); levamisole-resistant strains ZZ16 (lev-9(x16) X), CB211 (lev-1(e211) IV), the albendazole-resistant strain CB3474 (ben-1(e1880) III) and ivermectin-resistant strains VC722 (glc-2(ok1047) I) and DA1316 (avr-14(ad1302) I avr-15(ad1051) glc-1(pk54)). All strains were obtained from the Caenorhabditis Genetic Centre (CGC, Minneapolis, MN, USA). Strains were maintained at 20 °C on nematode growth medium (NGM) [62]. All worms were age-synchronized and obtained from eggs that were isolated by bleaching [63]. Aged-synchronous worms were then successfully grown in axenic medium ((3% (w/v) yeast extract, 3% (w/v) soy peptone, 1% (w/v) glucose, 0.5 mg/mL cholesterol and 0.5 mg/mL bovine hemoglobin) supplemented with 100 U/mL penicillin and 100 µg/mL streptomycin modified from the semi-define aqueous medium used by Chitwood and Feldlaufer [64] and incubated at 20 °C for worms to developed to L4 (or young adult stage). Due to the lack of an external food source, worms grew rather slowly at 20 °C [65,66].

4.4.2. In Vitro Screening: Lethality Testing Using Caenorhabditis elegans

Young adult (L4 stage) worms were used to assess drug lethality. Monitored endpoint was 48 h mortality. Tests were performed in 24-well plates. Five hundred (500) microliters of test solution, which is M9-buffer (3 g KH2PO4, 6 g Na2HPO4, 5 g NaCl, 0.25 g MgSO4.7H2O, in 1 L of water after been autoclaved supplemented with 2% glucose, 0.5% cholesterol), were distributed in 24-well plates. The volume of M9-buffer equivalent to the drug to be added for each drug final concentration (in each test, three to six concentrations were used) was removed. Untreated worms in M9-buffer were used as negative control. Positive controls were treated with levamisole, albendazole and ivermectin. Tested worms were treated with CE, fractions and pure compounds. Crude extract fraction (stock solution at 100 mg/mL) and pure compound (stock solution at 100 mg/mL) previously dissolved in M9-buffer were then added in well plates at increasing concentration (varying from 0 to 1000 or 1500 µg/mL for extract and fractions; 0 to 100 or 150 µg/mL). The young adult (10 to 15 ± 2) from the axenic culture (without food source) were then added at the test solution. The assay was incubated at 20 °C, and they were observed at each 24 h under binocular microscope for lethality determination. Dead and live worms were recorded 48 h post-exposure, then LC50 (lethal concentration of the extract or pure compound required to kill 50% of worms) was determined. Treatments were replicated three times. Worm mortality was investigated under microscope and worms was scored as dead if they were immotile, lacking movement following shaking or if they failed to move upon prodding with a worm pick and were fully elongated [66]. C. elegans with LC50 < 30 µg/mL of drug is highly sensitive; 30 < LC50 < 50 µg/mL is moderately sensitive; and 50 µg/mL < LC50 is less sensitive.

4.4.3. Onchocerca ochengi Isolation and Culture Conditions

• Male worms:
The adult male worms were isolated as previously describe by Ndjonka et al. [16] from fresh pieces of cattle skin (cow-udder) with palpable nodules obtained from the communal slaughter house of Ngaoundere in the Adamawa region of Cameroon. Nodules removed from the skin were brought to the laboratory, washed, drained and sterilized with 70% ethanol for dissection. After dissection of nodules, O. ochengi were extracted, isolated and washed three times in sterile phosphate-buffered saline (PBS). To analyze the anthelmintic activity of extract, fractions and pure compounds, the worms were incubated at 37 °C.
• Microfilaria (Mf):
Microfilariae were isolated as describe by Cho-Ngwa et al. and Ndjonka et al. [14,17]. About 10 skin snips were obtained from different locations of the skin. The skin snips were then incubated at 37 °C in PBS to let Mf emerge. The highly motile Mf were concentrated by centrifugation at 400× g for 10 min and quantified [22]. The Mf were incubated at 37 °C with the crude extract, fractions and pure compounds.
• Female worms:
The adult female worms were isolated by digestion of the nodules with collagenase at 37 °C. The female worms were cultured in a CO2-incubator [67]. For anthelmintic activity, they were incubated with the crude extracts, fractions and pure compounds.

4.4.4. In Vitro Assays: Lethality of Onchocerca ochengi

Adult worms (six individuals per well for males and one per well for the female) and Mf were washed twice and were subsequently transferred to RPMI-1640 medium supplemented with l-glutamine, 100 U/mL penicillin and 100 µg/mL streptomycin and incubated at 37 °C in 24-well plates (96-well plate for Mf) with different concentrations of the drugs tested. Worm mortality was checked by observation under the binocular microscope after 48 h [14,16,17]. After shaking, immotile and fully-elongated worms were considered to be dead. All tests were done in three independent triplicate determinations.

4.5. In Vitro Cytotoxicity and CC50 Determination

Cell culture: Cacao-2 cells at Passage 70 were kindly provided by Prof. Hensel, Institute of Pharmaceutical Biology and Phytochemistry, University of Münster, and were cultured as previously describe by Zumdick et al. [58]. Briefly, the cells from Passages 70 to 82 were grown in 75 cm2 culture flasks in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 4.5 g/L d-glucose, 2.2 g/L NaHCO3, 10% FBS, 1% non-essential amino acids, 100 IU/mL penicillin and 100 µg/mL streptomycin in an atmosphere of 5% CO2 and 95% relative humidity at 37 °C.
Cytotoxicity test: The cytotoxicity assays were performed according to the method of Rahman et al. [38]. Briefly, 1.5 × 104 viable cells/well were seeded in a 96-well plate and incubated for 24 to 48 h. Then, to initiate the experiments, the culture medium was replaced by fresh supplemented with drugs (increasing concentrations up to 100 or 150 µg/mL) dissolved in the medium for the tests. DMEM samples were used as the negative control. After 72 h of incubation, test solution was replaced by 30 µL of a 5 mg/mL 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) solution in each well and incubated at 37 °C in a 5% CO2 atmosphere with 95% humidity incubator for 4 h. The medium was removed, and formazan, generated by the activity of dehydrogenases, was solubilized with 70 µL of acidified isopropanol (0.4 M HCl). The amount of MTT-formazan that is directly proportional to the number of living cells was determined by measuring the optical density (OD) at 540 nm using a spectrophotometer. All controls used were treated equally. As control and to determine 100% growth, Caco-2 cells were grown in the absence of drugs so as to calculate cell viability. All determinations were performed in triplicate. The concentration of the drugs that killed 50% of the cells (CC50 50% cytotoxic concentration) was calculated [68]. The selectivity index (SI) values were calculated as the ratio: SI = CC50 of drug on Caco2/LC50 of drug on worm.

4.6. Acute Oral Toxicity on Rats

Adult Wistar rats (Rattus norvegicus), males aged 7 to 8 weeks and weighing 150 to 200 g, used for the experiments were obtained from LANAVET (Laboratoire National Vétérinaire, Garoua, Cameroon). The rats were acclimatized for one week in the Veterinary Research Laboratory of the Institute of Agricultural Research for Development, Wakwa, Cameroon, before initiating experiments. Animals were housed in groups of three (one group for the negative control, six groups for each drug at the first phase, six others at the second phase) under ambient temperatures (23 °C ± 1 °C) with relative humidity 55 ± 5% and 12 h light/dark cycle in polycarbonate cages. They were fed with standard rodent pellets and had unrestricted access to clean drinking water. They were monitored by a veterinarian for health status. There is no law yet regulating animal research in Cameroon [22]. All animal-related experimental procedures were approved by the regional delegation of Livestock, Fisheries and Animal industries (No.075/16/L/RA/DREPIA).
Acute oral toxicity (LD50, that is the dose that kills 50% of animals of the tested group) was measured using the method of the OECD guidelines for the testing of chemicals [56] following the European Community guidelines for the protection of animals used in experiments [69] and respecting the 3Rs (Replacement, Refinement, Reduction) [70]. Drugs in distilled water were orally administered separately at doses of 10 mg/kg, 100 mg/kg and 1000 mg/kg body weight. The negative controls were distilled water-administered rats. They were given to three groups of three rats each and observed for 24 h. Since no mortality was observed, the second phase was done with three groups of three rats each at drugs doses of 1500 mg/kg, 3000 mg/kg and 5000 mg/kg orally. The test was done at a single oral dose for each rat in a 5 mL/kg volume. The experimental animals were starved for 24 h before and 4 h after treatment. They were observed for 24 h; the number of deaths was recorded and the LD50 determined. The appearance of toxic symptoms, such as behavioral changes, locomotion, convulsions and mortality, was observed and recorded. Animals were observed constantly for the first 30 min after administration and, thereafter, every 4 h up to 24 h and, subsequently, once a day for up to 14 days.

4.7. Statistical Analysis

The LC50 values (lethal concentration of the extract or pure compound required to kill 50% of helminths) of all drugs were calculated using MS Excel. Positive controls were ivermectin, levamisole and albendazole. The CC50 values (50% cytotoxic concentration) of all drugs were calculated using GraphPad Prism 5.03. Data comparison was done using analysis of variances. Values were significant at p < 0.001.

5. Conclusions

Our results show that the polyphenol-rich fractions of A. nilotica containing mainly galloylated catechin and gallocatechin derivatives are potential anthelmintics. The reported ethnomedicinal use of this plant by traditional healers thus appears justified and receives a scientific basis. It is furthermore confirmed that the tested proanthocyanidins, most prominently ECG and EGCG, possess significant anthelmintic potency without noticeable adverse effects in animal experiments. This study provides the first evidence of a very high anti-Onchocerca activity of ECG and EGCG with LC50 values less than 2.5 µg/mL, i.e., in the same activity range as the positive controls levamisole, ivermectin and albendazole. These compounds and, possibly, further galloylated proanthocyanidins yet to be isolated from A. nilotica or other plant sources thus deserve further in-depth investigation as potential anthelmintics. In vivo study using the newly-validated Onchocerca ochengi microfilaricidal hamster model will be important to compare the similitude of the efficacy of in vitro and in vivo nematotoxicity.

Supplementary Materials

Supplementary materials are available online.

Acknowledgments

This research was supported by the fellowship of the German Academic Exchange Service (Deutscher Akademischer Austauschdienst) to J.V. Dikti. and the Alexander von Humboldt Foundation (AvH) to D. Ndjonka. This work is a part of the Ph.D. thesis of J.V. Dikti. We acknowledge the financial support from the Deutsche Forschungsgemeinschaft (DFG) grant LI 793/5-1 to the Cameroonian-German Cooperation Project (CGCP). We appreciate A. Hensel’s group from the Institute of Pharmaceutical Biology and Phytochemistry (IPBP) for the provision of the initial Caco-2 cell line cultures. The donation of samples of GC, EGC and EGCG by F. Petereit, IPBP, University of Münster, is gratefully acknowledged.

Author Contributions

D.N. and E.L. conceived of and designed the experiments and reviewed the manuscript. J.D.V. performed the experiments and wrote the manuscript. B.D. harvested plant material and analyzed the data. J.K. bought skin at the slaughterhouse and performed some experiments. T.J.S. contributed the laboratory facilities for the isolation and structural characterization, performed and interpreted the LC/MS analyses and reviewed the manuscript.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Organisation Mondiale de la Santé (OMS). Agir Pour Réduire L’Impact des Maladies Tropicales Négligées: Premier Rapport de L’OMS Sur les Maladies Tropicales Négligées; OMS: Genève, Switzerlands, 2011; pp. 124–129. [Google Scholar]
  2. Zoure, H.; Noma, M.; Tekle, A.; Amazigo, U.; Diggle, P.; Giorgi, E.; Remme, J.H. The geographic distribution of onchocerciasis in the 20 participating countries of the African Programme for Onchocerciasis Control: 2. pre-control endemicity levels and estimated number infected. Parasites Vectors 2014, 7, 326. [Google Scholar] [CrossRef] [PubMed]
  3. Tanya, V.N.; Wandji, S.; Kamgno, J.; Achukwi, D.M.; Enyong, P.A.I. Recent Advances in Onchocerciasis Research and Implication for Control; Cameroon Academy of Sciences: Yaounde, Cameroon, 2013; p. 200. [Google Scholar]
  4. Ali, M.M.; Mukhtar, M.M.; Baraka, O.Z.; Homeida, M.M.; Kheir, M.M.; Mackenzie, C.D. Immunocompetence may be important in the effectiveness of Mectizan (ivermectin) in the treatment of human onchocerciasis. Acta Trop. 2002, 84, 49–53. [Google Scholar] [CrossRef]
  5. Awadzi, K.; Boakye, D.A.; Edwards, G.; Opoku, N.O.; Attah, S.K.; Osei-Atweneboana, M.Y.; Lazdins-Helds, J.K.; Ardrey, A.E.; Addy, E.T.; Quartey, B.T.; et al. An investigation of persistent microfilaridermias despite multiple treatments with ivermectin, in two onchocerciasis-endemic foci in Ghana. Ann. Trop. Med. Parasitol. 2004, 98, 231–249. [Google Scholar] [CrossRef] [PubMed]
  6. Osei-Atweneboana, M.Y.; Eng, J.K.L.; Boakye, D.A.; Gyapong, J.O.; Prichard, R.K. Prevalence and intensity of Onchocerca volvulus infection and efficacy of ivermectin in endemic communities in Ghana: A twophase epidemiological study. Lancet 2007, 369, 2021–2029. [Google Scholar] [CrossRef]
  7. Langworthy, N.G.; Renz, A.; Mackenstedt, U.; Henkle-Dührsen, K.; de Bronsvoort, M.B.; Tanya, V.N.; Donnelly, M.J.; Trees, A.J. Macrofilaricidal activity of tetracycline against the filarial nematode Onchocerca ochengi: Elimination of Wolbachia precedes worm death and suggests a dependent relationship. Proc. Biol. Sci. 2000, 267, 1063–1069. [Google Scholar] [CrossRef] [PubMed]
  8. Townson, S.; Tagboto, S.; McGarry, H.F.; Egerton, G.L.; Taylor, M.J. Onchocerca parasites and Wolbachia endosymbionts: Evaluation of a spectrum of antibiotic types for activity against Onchocerca gutturosa in vitro. Filarial J. 2006, 5, 4. [Google Scholar] [CrossRef] [PubMed]
  9. Büttner, D.W.; Wanji, S.; Bazzocchi, C.; Bain, O.; Fischer, P. Obligatory symbiotic Wolbachia endobacteriae are absent from Loa loa. Filarial J. 2003, 2, 10. [Google Scholar] [CrossRef] [PubMed]
  10. Trees, A.J. Onchocerca ochengi: Mimic, Model, or Modulator of O. volvulus? Parasitol. Today 1992, 8, 337–339. [Google Scholar] [CrossRef]
  11. Ndjonka, D.; Rapado, L.N.; Silber, A.M.; Liebau, E.; Wrenger, C. Natural Products as a source for treating neglected parasitic diseases. Int. J. Mol. Sci. 2013, 14, 3395–3439. [Google Scholar] [CrossRef] [PubMed]
  12. Nyasse, B.; Ngantchou, I.; Nono, J.J.; Schneider, B. Antifilarial activity in vitro of polycarpol and 3-O-acetyl aleuritolic acid from cameroonian medicinal plants against Onchocercagutturosa. Nat. Prod. Res. 2006, 20, 391–397. [Google Scholar] [CrossRef] [PubMed]
  13. Ndjonka, D.; Abladam, E.D.; Djafsia, B.; Ajonina-Ekoti, I.; Achukwi, M.D.; Liebau, E. Anthelmintic activity of phenolic acids from the axlewood tree Anogeissus leiocarpus on the filarial nematode Onchocerca ochengi and drug-resistant strains of the free-living nematode Caenorhabditis elegans. J Helminthol. 2014, 88, 481–488. [Google Scholar] [CrossRef] [PubMed]
  14. Cho-Ngwa, F.; Abongwa, M.; Ngemenya, M.N.; Nyongbela, K.D. Selective activity of extracts of Margaritaria discoidea and Homalium africanum on Onchocerca ochengi. BMC Complement. Altern. Med. 2010, 10, 62. [Google Scholar] [CrossRef] [PubMed]
  15. Katiki, L.M.; Ferreira, J.F.; Gonzalez, J.M.; Zajac, A.M.; Lindsay, D.S.; Chagas, A.C.; Amarante, A.F. Anthelmintic effect of plant extracts containing condensed and hydrolyzable tannins on Caenorhabditis elegans, and their antioxidant capacity. Vet. Parasitol. 2012, 18, 218–227. [Google Scholar] [CrossRef] [PubMed]
  16. Ndjonka, D.; Agyare, C.; Luersen, K.; Djafsia, B.; Achukwi, D.; Nukenine, E.N.; Hensel, A.; Liebau, E. In Vitro activity of Cameroonian and Ghanaian medicinal plants on parasitic (Onchocerca ochengi) and free-living (Caenorhabditis elegans) nematodes. J. Helminthol. 2011, 85, 304–312. [Google Scholar] [CrossRef] [PubMed]
  17. Ndjonka, D.; Ajonina-Ekoti, I.; Djafsia, B.; Luersen, K.; Abladam, E.; Liebau, E. Anogeissus leiocarpus extract on the parasite nematode Onchocerca ochengi and on drug resistant mutantstrains of the free-living nematode Caenorhabditis elegans. Vet. Parasitol. 2012, 190, 136–142. [Google Scholar] [CrossRef] [PubMed]
  18. Thomsen, H.; Reider, K.; Franke, K.; Wessjohann, L.A.; Keiser, J.; Dagne, E.; Arnold, N. Characterization of constituents and anthelmintic properties of Hagenia abyssinica. Sci. Pharm. 2012, 80, 433–446. [Google Scholar] [CrossRef] [PubMed]
  19. Waterman, C.; Smith, R.A.; Pontiggia, L.; DerMarderosian, A. Anthelmintic screening of Sub-Saharan African plants used in traditional medicine. J. Ethnopharmacol. 2010, 127, 755–759. [Google Scholar] [CrossRef] [PubMed]
  20. Metuge, J.A.; Nyongbela, K.D.; Mbah, J.A.; Samje, M.; Fotso, G.; Babiaka, S.B.; Cho-Ngwa, F. Anti-Onchocerca activity and phytochemical analysis of an essential oil from Cyperus articulatus L. BMC Complement. Altern. Med. 2014, 14, 223. [Google Scholar] [CrossRef] [PubMed]
  21. Metuge, J.A.; Babiaka, S.B.; Mbah, J.A.; Ntie-Kang, F.; Ayimele, G.A.; Cho-Ngwa, F. Anti-onchocerca Metabolites from Cyperus articulatus: Isolation, In Vitro Activity and In Silico. ‘Drug-Likeness’. Nat. Prod. Bioprospect. 2014, 4, 243–249. [Google Scholar] [CrossRef] [PubMed]
  22. Samje, M.; Metuge, J.; Mbah, J.; Nguesson, B.; Cho-ngwa, F. In Vitro anti-Onchocerca ochengi activities of extracts and chromatographic fractions of Craterispermum laurinum and Morinda lucida. BMC Complement. Altern. Med. 2014, 14, 325. [Google Scholar] [CrossRef] [PubMed]
  23. Eyog, M.O.; Ndoye, O.; Kengue, J.; Awono, A. Les Fruitiers Forestiers Comestibles du Cameroun; International Plant Genetic Resources Institute (IPGRI): Cotonou, Benin, 2006; p. 220. [Google Scholar]
  24. Solomon-Wisdom, G.O.; Shittu, G.A. In Vitro antimicrobial and phytochemical activities of Acacia nilotica leaf extract. J. Med. Plants Res. 2010, 4, 1232–1234. [Google Scholar]
  25. Ali, A.; Akhtar, N.; Khan, B.A.; Khan, M.S.; Rasul, A.; Khalid, N.; Waseem, K.; Mahmood, T.; Ali, L. Acacia nilotica: A plant of multipurpose medicinal uses. J. Med. Plants Res. 2012, 6, 1492–1496. [Google Scholar]
  26. Maldini, M.; Montoro, P.; Hamed, A.I.; Mahalel, U.; Oleszek, W.; Stochmal, A.; Piacente, S. Strong antioxidant phenolics from Acacia nilotica: Profiling by ESI-MS and qualitative-quantitative determination by LC-ESI-MS. J. Pharm. Biomed. Anal. 2011, 56, 228–239. [Google Scholar] [CrossRef] [PubMed]
  27. Malan, E. Derivatives of (+)-catechin-5-gallate from the bark of Acacia nilotica. Phytochemistry 1991, 30, 2737–2739. [Google Scholar] [CrossRef]
  28. Singh, R.; Singh, B.; Singh, S.; Kumar, N.; Kumar, S.; Arora, S. Anti-free radical activities of kaempferol isolated from Acacia nilotica (L.) Willd. Ex. Del. Toxicol. In Vitro 2008, 22, 1965–1970. [Google Scholar] [CrossRef] [PubMed]
  29. Badar, N.; Iqbal, Z.; Khan, M.N.; Akhtar, M.S. In Vitro and In Vivo anthelmintic activity of Acacia nilotica (L.) willd. Ex Delile bark and leaves. Pak. Vet. J. 2011, 31, 185–191. [Google Scholar]
  30. Ferreira, L.E.; Castro, P.M.; Chagas, A.C.; França, S.C.; Beleboni, R.O. In vitro anthelmintic activity of aqueous leaf extract of Annona muricata L. (Annonaceae) against Haemonchus contortus from sheep. Exp. Parasitol. 2013, 134, 327–332. [Google Scholar] [CrossRef] [PubMed]
  31. Davis, A.L.; Cai, Y.; Davies, A.P.; Lewis, J.R. 1H and 13C NMR Assignments of some green tea polyphenols. Magn. Reson. Chem. 1996, 34, 887–890. [Google Scholar] [CrossRef]
  32. Ramanandraibe, V.; Grellier, P.; Martin, M.T.; Deville, A.; Joyeau, R.; Ramanitrahasimbola, D.; Mouray, E.; Rasoanaive, P.; Mambu, L. Antiplasmodial phenolic compounds from Piptadenia pervillei. Planta Med. 2008, 74, 417–421. [Google Scholar] [CrossRef] [PubMed]
  33. Kahiya, C.; Mukaratirwa, S.; Thamsborg, S.M. Effects of Acacia nilotica and Acacia karoo diets on Haemonchus contortus infection in goats. Vet. Parasitol. 2003, 115, 265–274. [Google Scholar] [CrossRef]
  34. Ahmadu, A.; Abdulkarim, A.; Grougnet, R.; Myrianthopoulos, V.; Tillequin, F.; Magiatis, P.; Skaltsounis, A.L. Two new peltogynoids from Acacia nilotica Delile with kinase inhibitory activity. Planta Med. 2010, 76, 458–460. [Google Scholar] [CrossRef] [PubMed]
  35. Geary, T.G.; Sangster, N.C.; Thompson, D.P. Frontiers in anthelmintic pharmacology. Vet. Parasitol. 1999, 84, 275–295. [Google Scholar] [CrossRef]
  36. Katiki, L.M.; Ferreira, J.F.; Zajac, A.M.; Masler, C.; Lindsay, D.S.; Carolina, A.; Chagas, A.C.; Amarante, A.F. Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use. Vet. Parasitol. 2011, 182, 264–268. [Google Scholar] [CrossRef] [PubMed]
  37. Koné, W.M.; Vargas, M.; Keiser, J. Anthelmintic activity of medicinal plants used in Côte d’Ivoire for treating parasitic diseases. Parasitol. Res. 2012, 110, 2351–2362. [Google Scholar] [CrossRef] [PubMed]
  38. Manolaraki, F.; Sotiraki, S.; Stefanakis, A.; Skampardonis, V.; Volanis, M.; Hoste, H. Anthelmintic activity of some Mediterranean browse plants against parasitic nematodes. Parasitology 2010, 137, 685–696. [Google Scholar] [CrossRef] [PubMed]
  39. Zhang, L.; Jie, G.; Zhang, J.; Zhao, B. Significant longevity-extending effects of EGCG on Caenorhabditis elegans under stress. Free Radic. Biol. Med. 2009, 46, 414–421. [Google Scholar] [CrossRef] [PubMed]
  40. Mukai, D.; Matsuda, A.E.N.; Yoshioka, A.E.Y. Potential anthelmintics: Polyphenols from the tea plant Camellia sinensis L. are lethally toxic to Caenorhabditis elegans. J. Nat. Med. 2008, 62, 155–159. [Google Scholar] [PubMed]
  41. Yamasaki, T.; Sato, M.; Mori, T.; Mohamed, A.S.A.; Fujii, K.; Tsukioka, J. Toxicity of tannins towards the free-living nematode Caenorhabditis elegans and the brine shrimp Artemia salina. J. Nat. Toxins 2002, 11, 165–171. [Google Scholar] [PubMed]
  42. Martin, R.J. Modes of Action of Anthelmintic Drugs. Vet. J. 1997, 31, 11–34. [Google Scholar] [CrossRef]
  43. Culetto, E.; Baylis, H.A.; Richmond, J.E.; Jones, A.K.; Fleming, J.T.; Squire, M.D.; Lewis, J.A.; Sattelle, D.B. The Caenorhabditis elegans unc-63 gene encodes a levamisole sensitive nicotinic acetylcholine. J. Biol. Chem. 2004, 279, 42476–42483. [Google Scholar] [CrossRef] [PubMed]
  44. Köhler, P. The biochemical basis of anthelmintic action and resistance. Int. J. Parasitol. 2001, 31, 336–345. [Google Scholar] [CrossRef]
  45. Roos, M.H.; Boersema, J.H.; Borgsteede, F.H.; Cornelissen, J.; Taylor, M.; Ruitenberg, E.J. Molecular analysis of selection for benzimidazole resistance in the sheep parasite Haemonchus contortus. Mol. Biochem. Parasitol. 1990, 43, 77–88. [Google Scholar] [CrossRef]
  46. Lubega, G.W.; Klein, R.D.; Geary, T.G.; Prichard, R.K. Haemonchus contortus: The role of two β-tubulin gene subfamilies in the resistance to benzimidazole anthelmintics. Biochem. Pharmacol. 1994, 47, 1705–1715. [Google Scholar] [CrossRef]
  47. Driscoll, M.; Dean, E.; Reilly, E.; Bergholz, E.; Chalfie, M. Genetic and Molecular Analysis of a Caenorhabditis elegans β-tubulin that conveys benzimidazole sensitivity. J. Cell Biol. 1989, 109, 2993–3003. [Google Scholar] [CrossRef] [PubMed]
  48. Yates, D.M.; Portillo, V.; Wolstenholme, A.J. The avermectin receptors of Haemonchus contortus and Caenorhabditis elegans. Int. J. Parasitol. 2003, 33, 1183–1193. [Google Scholar] [CrossRef]
  49. Laughton, D.L.; Lunt, G.G.; Wolstenholme, A.J. Reporter gene constructs suggest that the Caenorhabditis elegans avermectin receptor beta-subunit is expressed solely in the pharynx. J. Exp. Biol. 1997, 200, 1509–1514. [Google Scholar] [PubMed]
  50. Dent, J.A.; Smith, M.M.; Vassilatis, D.K.; Avery, L. The genetics of ivermectin resistance in Caenorhabditis elegans. Proc. Natl. Acad. Sci. USA 2000, 97, 2674–2679. [Google Scholar] [CrossRef] [PubMed]
  51. Chun-Yang, Y. Emerging usage of plant-based coagulants for water and wastewater treatment. Process Biochem. 2010, 45, 1437–1444. [Google Scholar]
  52. Jeon, J.R.; Kim, E.J.; Kim, Y.M.; Murugesan, K.; Kim, J.H.; Chang, Y.S. Use of grape seed and its natural polyphenol extracts as a natural organic coagulant for removal of cationic dyes. Chemosphere 2009, 77, 1090–1098. [Google Scholar] [CrossRef] [PubMed]
  53. Iqbal, Z.; Sarwar, M.; Jabbar, A.; Ahmed, S.; Nisa, M.; Sajid, M.S.; Khan, M.N.; Mufti, K.A.; Yaseen, M. Direct and indirect anthelmintic effects of condensed tannins in sheep. Vet. Parasitol. 2007, 144, 125–131. [Google Scholar] [CrossRef] [PubMed]
  54. Thompson, D.P.; Geary, T.G. The structure and function of helminth surfaces. In Biochemistry and Molecular Biology of Parasites, 1st ed.; Marr, J.J., Ed.; Academic Press: New York, NY, USA, 1995; Volume 1, pp. 203–232. [Google Scholar]
  55. Organization for Economic Co-operation and Development (OECD). Guideline No. 423. Acute Oral Toxicity in Animals; OECD/OCDE No. 423; OECD: Paris, France, 2001. [Google Scholar]
  56. Mohan, S.; Thiagarajan, K.; Chandrasekaran, R.; Arul, J. In Vitro protection of biological macromolecules against oxidative stress and in vivo toxicity evaluation of Acacia nilotica (L.) and ethyl gallate in rats. BMC Complement. Altern. Med. 2014, 14, 257. [Google Scholar] [CrossRef] [PubMed]
  57. Guta, M.; Urga, K.; Assefa, A.; Lemma, H.; Addis, G.; Gemeda, N.; Yirsaw, K.; Mudi, K.; Melaku, D. Antibacterial and acute toxicity study of Acacia nilotica. Ethiop. J. Biol. Sci. 2007, 6, 43–49. [Google Scholar]
  58. Zumdick, S.; Deters, A.; Hensel, A. In vitro transport of Oligomeric Procyanidins (DP 2 to 4) across monolayers caco-2 cells. Fitoterapia 2012, 83, 1210–1217. [Google Scholar] [CrossRef] [PubMed]
  59. Huggins, D.J.; Sherman, W.; Tidor, B. Rational Approaches to Improving Selectivity in Drug Design. J. Med. Chem. 2012, 55, 1424–1444. [Google Scholar] [CrossRef] [PubMed]
  60. Qa’dan, F.; Petereit, F.; Nahrstedt, A. Prodelphinidin trimers and characterization of a proanthocyanidin oligomer from Cistus albidus. Pharmazie 2003, 58, 416–419. [Google Scholar] [PubMed]
  61. Danne, A.; Petereit, F.; Nahrstedt, A. Flavan-3-ols, prodelphinidins and further polyphenols from Cistus salvifolius. Phytochemistry 1994, 37, 533–538. [Google Scholar] [CrossRef]
  62. Brenner, S. The genetics of Caenorhabditis elegans. Genetics 1974, 77, 71–94. [Google Scholar] [PubMed]
  63. Sulston, J.; Hodgkin, J. Methods. In The Nematode Caenorhabditis elegans, 13th ed.; Wood, W.B., Ed.; Cold Spring Harbor Laboratory: Cold Spring Harbor, NY, USA, 1988; Volume 17, pp. 587–606. [Google Scholar]
  64. Chitwood, D.J.; Feldlaufer, M.F. Ecdysteroids in Axenically Propagated Caenorhabditis elegans and medium culture medium. J. Nematol. 1990, 22, 598–607. [Google Scholar] [PubMed]
  65. Dengg, M.; van Meel, J.C. Caenorhabditis elegans as model system for rapid toxicity assessment of pharmaceutical compounds. J. Pharmacol. Toxicol. Methods 2004, 50, 209–214. [Google Scholar] [CrossRef] [PubMed]
  66. Qiao, Y.; Zhao, Y.; Wu, Q.; Sun, L.; Ruan, Q.; Chen, Y.; Wang, M.; Duan, J.; Wang, D. Full toxicity assessment of Genkwa Flos and the underlying mechanism in nematode Caenorhabditis elegans. PLoS ONE 2014, 9, e91825. [Google Scholar] [CrossRef] [PubMed]
  67. Kläger, S.; Whitworth, J.A.G.; Post, R.J.; Chavasse, D.C.; Dowham, M.D. How long do the effects of ivermectin on adult Onchocerca volvulus persist? Trop. Med. Pasatol. 1993, 44, 305–310. [Google Scholar]
  68. Ajaiyeoba, E.O.; Abiodun, O.O.; Falade, M.O.; Ogbole, N.O.; Ashidi, J.S.; Happi, C.T.; Akinboye, D.O. In Vitro cytotoxicity studies of 20 plants used in Nigerian antimalarial ethnomedicine. Phytomedicine 2006, 13, 295–298. [Google Scholar] [CrossRef] [PubMed]
  69. European Economic Community. Council Directive 86/609/EEC of 24 November 1986 on the Approximation of Laws, Regulations and Administrative Provisions of the Member States Regarding the Protection of Animals Used for Experimental and Other Scientific Purposes; European Economic Community: Brussels, Belgium, 1986. [Google Scholar]
  70. Kilkenny, C.; Browne, W.J.; Cuthill, I.C.; Emerson, M.; Altman, D.G. Improving bioscience research reporting: The ARRIVE guidelines for reporting animal research. PLoS Biol. 2010, 8, e100041. [Google Scholar] [CrossRef] [PubMed]
  • Sample Availability: Not available.
Figure 1. UHPLC/+ESI QqTOF MS (Ultra-high performance liquid chromatography positive electrospray ionization quadrupole/time-of-flight mass spectrometer) analysis of Fraction 9. (a): base peak chromatogram (m/z 100 to 1000); (b): extracted ion chromatograms for m/z 443 (blue), 291 (green), 458 (red), 307 (magenta). For peak assignment, see Figure 3.
Figure 1. UHPLC/+ESI QqTOF MS (Ultra-high performance liquid chromatography positive electrospray ionization quadrupole/time-of-flight mass spectrometer) analysis of Fraction 9. (a): base peak chromatogram (m/z 100 to 1000); (b): extracted ion chromatograms for m/z 443 (blue), 291 (green), 458 (red), 307 (magenta). For peak assignment, see Figure 3.
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Figure 2. +ESI QqTOF MS spectra of Peaks 1 to 8 (see Figure 2). Assignment of spectra: (1) gallocatechin (GC); (2) catechin; (3) gallocatechin-3′- or 4′-O-gallate; (4) gallocatechin-7- or 5-O-gallate; (5) gallocatechin-3-O-gallate; (6) catechin-O-gallate (or gallocatechin-protocatechuate); (7) catechin-3-O-gallate (CG); (8) catechin-di-O-gallate. Peak 7 represents the isolated catechin-3-O-gallate.
Figure 2. +ESI QqTOF MS spectra of Peaks 1 to 8 (see Figure 2). Assignment of spectra: (1) gallocatechin (GC); (2) catechin; (3) gallocatechin-3′- or 4′-O-gallate; (4) gallocatechin-7- or 5-O-gallate; (5) gallocatechin-3-O-gallate; (6) catechin-O-gallate (or gallocatechin-protocatechuate); (7) catechin-3-O-gallate (CG); (8) catechin-di-O-gallate. Peak 7 represents the isolated catechin-3-O-gallate.
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Figure 3. Chemical structures of the proanthocyanidins tested for anthelminthic activity.
Figure 3. Chemical structures of the proanthocyanidins tested for anthelminthic activity.
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Table 1. LC50 of Acacia nilotica crude extract and pure proanthocyanidin derivatives at 48 h post-treatment against O. ochengi and C. elegans (wild type and drug-resistant strains).
Table 1. LC50 of Acacia nilotica crude extract and pure proanthocyanidin derivatives at 48 h post-treatment against O. ochengi and C. elegans (wild type and drug-resistant strains).
LC50/48 h in μg/mL (µM)
WormsCECGECGGCEGCEGCGLevamisoleIvermectinAlbendazole
Means LC50 ± SD
O. ochengi males11.5 ± 0.17.6 ± 0.2 (17.2)2.1 ± 0.3 (4.7)4.2 ± 0.1 (13.6)2.1 ± 0.4 (6.8)1.2 ± 0.5 (2.6)1.0 ± 1.0 (5.1)1.2 ± 0.5 (1.3)4.2 ± 0.3 (15.7)
O. ochengi females11.0 ± 0.24.5 ± 0.3 (10.2)2.1 ± 0.6 (4.7)5.5 ± 0.3 (18.0)3.3 ± 0.6 (10.6)1.0 ± 0.5 (2.3)2.1 ± 0.1 (10.2)1.5 ± 0.3 (1.7)1.0 ± 0.2 (3.9)
O. ochengi microfilariae10.8 ± 0.34.2 ± 0.1 (9.4)1.0 ± 0.4 (2.2)3.2 ± 0.3 (10.4)3.3 ± 0.5 (10.6)1.3 ± 0.2 (2.8)1.0 ± 0.3 (5.1)1.5 ± 0.0 (1.7)2.1 ± 0.5 (7.8)
C. elegans WT350 ± 1.190.6 ± 0.07 (204.8)33.8 ± 2.7 (2.4)74.3 ± 0.3 (242.4)62.7 ± 0.2 (204.7)49.8 ± 0.8 (108.6)1.4 ± 0.2 (7.2)1.2 ± 0.1 (1.3)7.1 ± 0,2 (26.4)
C. elegans ZZ16987.9 ± 0.395.3 ± 0.1 (215.4)39.5 ± 0.9 (76.4)65.6 ± 0.2 (214.2)92.0 ± 0.3 (301.4)>100>100ndnd
C. elegans CB211934.3 ± 1.096.4 ± 0.1 (217.9)41.7 ± 1.2 (89.3)21.5 ± 0.3 (70.2)83.3 ± 0.6 (271.9)26.3 ± 1.3 (57.4)>100ndnd
C. elegans CB3474982.9 ± 0.695.2 ± 0.3 (215.2)19.6 ± 0.8 (44.2)83.1 ± 0.1 (271.4)53.8 ± 0.9 (175.7)35.7 ± 1.0 (77.9)ndnd>100
C. elegans VC722985.5 ± 0.791.8 ± 0.3 (207.5)27.2 ± 1.4 (61.4)96.3 ± 0.07 (314.3)93.8 ± 0.3 (306.3)43.7 ± 2.1 (95.4)nd>100nd
C. elegans DA1316999.3 ± 1.296.3 ± 0.3 (217.7)27.4 ± 1.1 (61.9)82.5 ± 0.1 (269.4)87.5 ± 0.5 (285.7)40.8 ± 0.3 (89.1)nd>100nd
Each value represents mean ± SD (standard deviation); values between brackets are LC50 after 48 h calculated in µM; nd = not determined; CE: crude extract; CG: catechin-3-O-gallate; ECG: epicatechin-3-O-gallate; GC: gallocatechin; EGC: epigallocatechin; EGCG: epigallocatechin-3-O-gallate.
Table 2. Cytotoxicity activity of pure compounds and crude extract from A. nilotica fruits and positive controls on Caco-2 cell lines. Selectivity index (SI) on C. elegans wild-type, O. ochengi males, females and microfilariae.
Table 2. Cytotoxicity activity of pure compounds and crude extract from A. nilotica fruits and positive controls on Caco-2 cell lines. Selectivity index (SI) on C. elegans wild-type, O. ochengi males, females and microfilariae.
CompoundsCytotoxicity μg/mL (μM)Selectivity Index (SI)
Means CC50 ± SD
Caco-2C. elegansO. ochengi
Wild TypeMalesFemalesMicrofilariae
CE93.2 ± 1.10.38.18.58.6
CG66.3 ± 0.6 (149.9)0.78.714.715.8
ECG67.6 ± 0.5 (152.8)2.032.232.267.6
GC66.7 ± 0.7 (217.8)0.915.912.120.8
EGC47.1 ± 0.5 (153.8)0.822.414.314.3
EGCG60.9 ± 0.8 (132.9)1.250.860.946.9
Levamisole27.3 ± 0.8 (31.2)18.827.313.027.3
Ivermectin28.7 ± 0.7 (140.5)23.923.919.119.1
Albendazole29.4 ± 0.5 (110.8)4.1729.414
Each value of cytotoxicity represents mean ± SD (standard deviation); values between brackets are CC50 after 48 h calculated in µM. Selectivity index (SI) = CC50 on mammalian cells/LC50 on worm.

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