Altholactone Inhibits NF-κB and STAT3 Activation and Induces Reactive Oxygen Species-Mediated Apoptosis in Prostate Cancer DU145 Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Altholactone-Induced Cell Growth Inhibition in Prostate Cancer Cells
2.2. Effect of Altholactone on Induction of DU145 Cell Cycle
2.3. Effect of Altholactone on Induction of DU145 Cell Apoptosis
2.4. Altholactone Promoted ROS Generation and Inhibited NF-κB Activity in DU145 Cells
2.5. Altholactone Inhibited Constitutive, IL6- and STAT3C-Induced STAT3 Transcriptional Activity and STAT3 Phosphorylation in DU145 Cells
2.6. Effect of Alotholactone on STAT3 Gene Products and on Apoptosis-Related Proteins
3. Materials and Methods
3.1. Chemical, Plasmid Constructsand Other Reagents
3.2. Cell Culture
3.3. Cell Proliferation Assay
3.4. Flow Cytometric Determination of Cell Cyle Propagation
3.5. Flow Cytometric Determination of Apoptosis
3.6. Flow Cytometric Determination of Reactive Oxygen Species (ROS) in DU145 Cells
3.7. Trasient Transfection and Luciferase Assays
3.8. Western Blotting
3.9. Statistical Analysis of Data
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Jiang, C.; Masood, M.; Rasul, A.; Wei, W.; Wang, Y.; Ali, M.; Mustaqeem, M.; Li, J.; Li, X. Altholactone Inhibits NF-κB and STAT3 Activation and Induces Reactive Oxygen Species-Mediated Apoptosis in Prostate Cancer DU145 Cells. Molecules 2017, 22, 240. https://doi.org/10.3390/molecules22020240
Jiang C, Masood M, Rasul A, Wei W, Wang Y, Ali M, Mustaqeem M, Li J, Li X. Altholactone Inhibits NF-κB and STAT3 Activation and Induces Reactive Oxygen Species-Mediated Apoptosis in Prostate Cancer DU145 Cells. Molecules. 2017; 22(2):240. https://doi.org/10.3390/molecules22020240
Chicago/Turabian StyleJiang, Chunwa, Muqaddas Masood, Azhar Rasul, Wei Wei, Ya Wang, Muhammad Ali, Muhammad Mustaqeem, Jiang Li, and Xiaomeng Li. 2017. "Altholactone Inhibits NF-κB and STAT3 Activation and Induces Reactive Oxygen Species-Mediated Apoptosis in Prostate Cancer DU145 Cells" Molecules 22, no. 2: 240. https://doi.org/10.3390/molecules22020240