Next Article in Journal
A Water Cluster Conduit in Crystal
Previous Article in Journal
Activation of Recombinantly Expressed l-Amino Acid Oxidase from Rhizoctonia solani by Sodium Dodecyl Sulfate
Article

Optimization Technology of the LHS-1 Strain for Degrading Gallnut Water Extract and Appraisal of Benzene Ring Derivatives from Fermented Gallnut Water Extract Pyrolysis by Py-GC/MS

by 1,2,3,4,5,* and 1,2,3,4,5
1
Institute of Chemical Industry of Forest Products, Chinese Academy Forestry, Nanjing 210042, China
2
National Engineering Laboratory for Biomass Chemical Utilization, Nanjing 210042, China
3
Key and Open Laboratory on Forest Chemical Engineering, State Forestry Administration, Nanjing 210042, China
4
Key Laboratory of Biomass Energy and Material, Nanjing 210042, China
5
Institute of New Technology of Forestry, Chinese Academy Forestry, Beijing 100091, China
*
Author to whom correspondence should be addressed.
Molecules 2017, 22(12), 2253; https://doi.org/10.3390/molecules22122253
Received: 31 October 2017 / Revised: 9 December 2017 / Accepted: 11 December 2017 / Published: 20 December 2017
(This article belongs to the Section Chemical Biology)
Gallnut water extract (GWE) enriches 80~90% of gallnut tannic acid (TA). In order to study the biodegradation of GWE into gallic acid (GA), the LHS-1 strain, a variant of Aspergillus niger, was chosen to determine the optimal degradation parameters for maximum production of GA by the response surface method. Pyrolysis–gas chromatography–mass spectrometry (Py-GC/MS) was first applied to appraise benzene ring derivatives of fermented GWE (FGWE) pyrolysis by comparison with the pyrolytic products of a tannic acid standard sample (TAS) and GWE. The results showed that optimum conditions were at 31 °C and pH of 5, with a 50-h incubation period and 0.1 g·L−1 of TA as substrate. The maximum yields of GA and tannase were 63~65 mg·mL−1 and 1.17 U·mL−1, respectively. Over 20 kinds of compounds were identified as linear hydrocarbons and benzene ring derivatives based on GA and glucose. The key benzene ring derivatives were 3,4,5-trimethoxybenzoic acid methyl ester, 3-methoxy-1,2-benzenediol, and 4-hydroxy-3,5-dimethoxy-benzoic acid hydrazide. View Full-Text
Keywords: gallnut water extract; tannic acid; benzene ring derivatives; biodegradation; Py-GC/MS gallnut water extract; tannic acid; benzene ring derivatives; biodegradation; Py-GC/MS
Show Figures

Figure 1

MDPI and ACS Style

Wang, C.; Li, W. Optimization Technology of the LHS-1 Strain for Degrading Gallnut Water Extract and Appraisal of Benzene Ring Derivatives from Fermented Gallnut Water Extract Pyrolysis by Py-GC/MS. Molecules 2017, 22, 2253. https://doi.org/10.3390/molecules22122253

AMA Style

Wang C, Li W. Optimization Technology of the LHS-1 Strain for Degrading Gallnut Water Extract and Appraisal of Benzene Ring Derivatives from Fermented Gallnut Water Extract Pyrolysis by Py-GC/MS. Molecules. 2017; 22(12):2253. https://doi.org/10.3390/molecules22122253

Chicago/Turabian Style

Wang, Chengzhang; Li, Wenjun. 2017. "Optimization Technology of the LHS-1 Strain for Degrading Gallnut Water Extract and Appraisal of Benzene Ring Derivatives from Fermented Gallnut Water Extract Pyrolysis by Py-GC/MS" Molecules 22, no. 12: 2253. https://doi.org/10.3390/molecules22122253

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop