Next Article in Journal
Dietary Consumption of Phenolic Acids and Prostate Cancer: A Case-Control Study in Sicily, Southern Italy
Next Article in Special Issue
Improved Performance of Magnetic Cross-Linked Lipase Aggregates by Interfacial Activation: A Robust and Magnetically Recyclable Biocatalyst for Transesterification of Jatropha Oil
Previous Article in Journal
Anti-Candida Activity of Bursera morelensis Ramirez Essential Oil and Two Compounds, α-Pinene and γ-Terpinene—An In Vitro Study
Previous Article in Special Issue
Lipoprotein Lipase Expression in Chronic Lymphocytic Leukemia: New Insights into Leukemic Progression
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessArticle
Molecules 2017, 22(12), 2153;

Frozen Microemulsions for MAPLE Immobilization of Lipase

Istituto Motori-CNR, via G. Marconi 8, 80125 Napoli, Italy
CNR-SPIN and Department of Physics “Ettore Pancini”, University of Naples Federico II, Piazzale V. Tecchio 80, 80125 Napoli, Italy
Department of Chemical Engineering, Materials and Industrial Production, University of Naples Federico II, Piazzale V. Tecchio 80, 80125 Napoli, Italy
Author to whom correspondence should be addressed.
Received: 21 September 2017 / Revised: 26 November 2017 / Accepted: 27 November 2017 / Published: 5 December 2017
(This article belongs to the Special Issue Lipases and Lipases Modification)
Full-Text   |   PDF [3194 KB, uploaded 6 December 2017]   |  


Candida rugosa lipase (CRL) was deposited by matrix assisted pulsed laser evaporation (MAPLE) in order to immobilize the enzyme with a preserved native conformation, which ensures its catalytic functionality. For this purpose, the composition of the MAPLE target was optimized by adding the oil phase pentane to a water solution of the amino acid 3-(3,4-dihydroxyphenyl)-2-methyl-l-alanine (m-DOPA), giving a target formed by a frozen water-lipase-pentane microemulsion. Fourier transform infrared (FTIR) spectroscopy and atomic force microscopy (AFM) were used to investigate the structure of MAPLE deposited lipase films. FTIR deconvolution of amide I band indicated a reduction of unfolding and aggregation, i.e., a better preserved lipase secondary structure in the sample deposited from the frozen microemulsion target. AFM images highlighted the absence of big aggregates on the surface of the sample. The functionality of the immobilized enzyme to promote transesterification was determined by thin layer chromatography, resulting in a modified specificity. View Full-Text
Keywords: MAPLE; microemulsion; lipase; thin film; enzyme immobilization MAPLE; microemulsion; lipase; thin film; enzyme immobilization

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Califano, V.; Bloisi, F.; Perretta, G.; Aronne, A.; Ausanio, G.; Costantini, A.; Vicari, L. Frozen Microemulsions for MAPLE Immobilization of Lipase. Molecules 2017, 22, 2153.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top