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Molecules 2017, 22(12), 2118;

Optimization of Bioactive Polyphenols Extraction from Picea Mariana Bark

Renewable Materials Research Centre, Department of Wood and Forest Sciences, Université Laval, Québec, QC G1V 0A6, Canada
Institute of Nutrition and Functional Food (INAF), Université Laval, Quebec, QC G1V 0A6, Canada
Author to whom correspondence should be addressed.
Received: 30 October 2017 / Revised: 30 November 2017 / Accepted: 30 November 2017 / Published: 1 December 2017
(This article belongs to the Collection Bioactive Compounds)
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Reported for its antioxidant, anti-inflammatory and non-toxicity properties, the hot water extract of Picea mariana bark was demonstrated to contain highly valuable bioactive polyphenols. In order to improve the recovery of these molecules, an optimization of the extraction was performed using water. Several extraction parameters were tested and extracts obtained analyzed both in terms of relative amounts of different phytochemical families and of individual molecules concentrations. As a result, low temperature (80 °C) and low ratio of bark/water (50 mg/mL) were determined to be the best parameters for an efficient polyphenol extraction and that especially for low molecular mass polyphenols. These were identified as stilbene monomers and derivatives, mainly stilbene glucoside isorhapontin (up to 12.0% of the dry extract), astringin (up to 4.6%), resveratrol (up to 0.3%), isorhapontigenin (up to 3.7%) and resveratrol glucoside piceid (up to 3.1%) which is here reported for the first time for Picea mariana. New stilbene derivatives, piceasides O and P were also characterized herein as new isorhapontin dimers. This study provides novel information about the optimal extraction of polyphenols from black spruce bark, especially for highly bioactive stilbenes including the trans-resveratrol. View Full-Text
Keywords: Picea mariana; bark; hot water extraction; chemometrics; stilbenes; piceaside Picea mariana; bark; hot water extraction; chemometrics; stilbenes; piceaside

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Francezon, N.; Meda, N.-S.-B.; Stevanovic, T. Optimization of Bioactive Polyphenols Extraction from Picea Mariana Bark. Molecules 2017, 22, 2118.

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