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Molecules 2017, 22(11), 2025;

Application of Enzymatic Extracts from a CALB Standard Strain as Biocatalyst within the Context of Conventional Biodiesel Production Optimization

Department of Organic Chemistry, University of Cordoba (UCO), Cordoba 14014, Spain
Seneca Green Catalyst S.L., Rabanales XXI/University of Cordoba, Cordoba 14014, Spain
Department of Microbiology, University of Cordoba, Campus de Rabanales, Ed. Severo Ochoa, 14014 Cordoba, Spain
Author to whom correspondence should be addressed.
Received: 30 October 2017 / Revised: 16 November 2017 / Accepted: 18 November 2017 / Published: 21 November 2017
(This article belongs to the Special Issue Meeting of the Spanish Catalysis Society (SECAT’17))
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The application of biocatalysts in the transesterification process of triglycerides (TG) allows integrating the glycerol in the form of monoglyceride (MG), sharply increasing the yield and the environmental sustainability of the conventional biodiesel production process. This is known as Ecodiesel. To overcome the inconvenient of the high cost of the currently employed highly purified commercial enzymes, the use of scarcely purified extracts obtained from standard strains of the same species of commercial lipases currently applied in this process is being investigated. Thus, Candida antarctica type B (CALB) was chosen to determine the optimal conditions of culture of this yeast. The standard strain was obtained from the Spanish Type Microbial Cultures Collection (CECT) and has been used to carry out several studies to elucidate its optimum growth conditions. Through a process of lyophilization with prior dialysis of the liquid cultures, the enzymatic extracts were obtained. The different obtained cultures have been applied as biocatalysts in the 1,3-selective transesterification reaction of sunflower oil with ethanol to obtain Ecodiesel (FAEE + MG). Selectivity and reaction yields were obtained by gas chromatography. Acceptable yields are obtained during the reaction time as well as in successive reactions, demonstrating the feasibility of using these CALB enzymatic extracts as biocatalysts. View Full-Text
Keywords: biodiesel; ecodiesel; lipases; CALB; enzymatic extracts; transesterification; glycerol biodiesel; ecodiesel; lipases; CALB; enzymatic extracts; transesterification; glycerol

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Luna, C.; Luna, D.; Bautista, F.M.; Estevez, R.; Calero, J.; Posadillo, A.; Romero, A.A.; Sancho, E.D. Application of Enzymatic Extracts from a CALB Standard Strain as Biocatalyst within the Context of Conventional Biodiesel Production Optimization. Molecules 2017, 22, 2025.

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