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Molecules 2017, 22(11), 1974;

Larix decidua Bark as a Source of Phytoconstituents: An LC-MS Study

Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35131 Padova, Italy
Unifarco spa, Via Cal Longa 62, Santa Giustina 32035 Belluno, Italy
Unired srl, Via N. Tommaseo 69, 35131 Padova, Italy
Author to whom correspondence should be addressed.
Received: 6 October 2017 / Revised: 10 November 2017 / Accepted: 14 November 2017 / Published: 15 November 2017
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Larix decidua bark is a waste of the timber industry and is widely diffused in Northern Italy. This material can be considered a good source of antioxidants and phytoconstituents with possible use in cosmetic or nutraceutical products. In this study, simple extraction of larch bark was performed using mixtures of ethanol/water. Furthermore, the phytochemical composition of larch bark extract was studied using LC-MSn methods and the main constituents were identified as flavonoids, spiro-polyphenols, and procyanidins. To confirm the identification by LC-MS semi-preparative HPLC was performed in order to isolate the main constituents and verify the structures by 1H-NMR. Antioxidant properties were studied using an in vitro approach combining DPPH assay and LC-MS in order to establish different roles of the various classes of phytochemicasl of the extract. DPPH activity of some of the isolated compounds was also assessed. The overall results indicate this waste material as a good source of antioxidant compounds, mainly procyanidins, whichresulted the most active constituents in the DPPH assay. View Full-Text
Keywords: Larix decidua bark; procyanidins; spiro-polyphenol; DPPH; LC-MS; flavonoids; polyphenols Larix decidua bark; procyanidins; spiro-polyphenol; DPPH; LC-MS; flavonoids; polyphenols

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Baldan, V.; Sut, S.; Faggian, M.; Dalla Gassa, E.; Ferrari, S.; De Nadai, G.; Francescato, S.; Baratto, G.; Dall’Acqua, S. Larix decidua Bark as a Source of Phytoconstituents: An LC-MS Study. Molecules 2017, 22, 1974.

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