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Open AccessArticle

Lipase-Catalyzed Transesterification of Egg-Yolk Phophatidylcholine with Concentrate of n-3 Polyunsaturated Fatty Acids from Cod Liver Oil

Department of Chemistry, Wroclaw University of Environmental and Life Sciences, Norwida 25, 50-375 Wroclaw, Poland
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Molecules 2017, 22(10), 1771; https://doi.org/10.3390/molecules22101771
Received: 29 September 2017 / Revised: 16 October 2017 / Accepted: 18 October 2017 / Published: 19 October 2017
(This article belongs to the Special Issue Lipases and Lipases Modification)
Phospholipids containing PUFAs are important vehicles for their delivering to the targeted tissues. In our research project we established enzymatic methods for the enrichment of natural egg-yolk PC with n-3 PUFAs. Instead of synthetic PUFA ethyl esters, the new strategy was developed using polyunsaturated fatty acids enriched fraction (PUFA-EF) from cod liver oil as the natural acyl donors. PUFA-EF was produced by urea-complexation and contained 86.9% PUFA including 8.5% stearidonic acid (SDA; 18:4(n-3)), 26.7% EPA, and 45.2% DHA. The transesterification of PC with PUFA was catalyzed by lipases. After screening of enzymes the effect of reaction medium; molar ratio of substrates and etc. was investigated. The highest incorporation of PUFA was 45.6%; including 36.8% DHA and 5.8% EPA at the following reaction conditions: hexane; 55 °C; PUFA-EF/PC acyl ratio of 10; 48 h of reaction time and lipase B from Candida antarctica as a biocatalyst (20% of enzyme load). View Full-Text
Keywords: PUFA-enriched phospholipids; lipases; phospholipids transesterification; n-3 PUFA PUFA-enriched phospholipids; lipases; phospholipids transesterification; n-3 PUFA
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Chojnacka, A.; Gładkowski, W.; Grudniewska, A. Lipase-Catalyzed Transesterification of Egg-Yolk Phophatidylcholine with Concentrate of n-3 Polyunsaturated Fatty Acids from Cod Liver Oil. Molecules 2017, 22, 1771.

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