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Molecules 2016, 21(9), 1163;

Small Molecule-Photoactive Yellow Protein Labeling Technology in Live Cell Imaging

Powder Metallurgy Research Institute of Central South University, Changsha 410013, China
The Third Xiangya Hospital, Central South University, Changsha 410013, China
School of Pharmaceutical Sciences, Central South University, Changsha 410013, China
Authors to whom correspondence should be addressed.
Academic Editors: Zhen Cheng and Diego Muñoz-Torrero
Received: 1 July 2016 / Revised: 9 August 2016 / Accepted: 16 August 2016 / Published: 31 August 2016
(This article belongs to the Special Issue Molecular Imaging Probes)
Full-Text   |   PDF [2394 KB, uploaded 31 August 2016]   |  


Characterization of the chemical environment, movement, trafficking and interactions of proteins in live cells is essential to understanding their functions. Labeling protein with functional molecules is a widely used approach in protein research to elucidate the protein location and functions both in vitro and in live cells or in vivo. A peptide or a protein tag fused to the protein of interest and provides the opportunities for an attachment of small molecule probes or other fluorophore to image the dynamics of protein localization. Here we reviewed the recent development of no-wash small molecular probes for photoactive yellow protein (PYP-tag), by the means of utilizing a quenching mechanism based on the intramolecular interactions, or an environmental-sensitive fluorophore. Several fluorogenic probes have been developed, with fast labeling kinetics and cell permeability. This technology allows quick live-cell imaging of cell-surface and intracellular proteins without a wash-out procedure. View Full-Text
Keywords: small molecule; photo active yellow protein; live cell; protein labeling; imaging small molecule; photo active yellow protein; live cell; protein labeling; imaging

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Gao, F.; Gao, T.; Zhou, K.; Zeng, W. Small Molecule-Photoactive Yellow Protein Labeling Technology in Live Cell Imaging. Molecules 2016, 21, 1163.

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