Next Article in Journal
Immunomodulatory and Antioxidant Effects of Polysaccharides from Gynostemma pentaphyllum Makino in Immunosuppressed Mice
Next Article in Special Issue
Chemical Composition, Antioxidant, and Antibacterial Activity of Wood Vinegar from Litchi chinensis
Previous Article in Journal
Porphyromonas gingivalis Lipopolysaccharide Induced Proliferation and Activation of Natural Killer Cells in Vivo
Previous Article in Special Issue
Siegesbeckia orientalis Extract Inhibits TGFβ1-Induced Migration and Invasion of Endometrial Cancer Cells
Article Menu
Issue 8 (August) cover image

Export Article

Open AccessArticle
Molecules 2016, 21(8), 1092;

Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies

School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China
State Key Laboratory of Food Science and Technology, Sino-Germany Joint Research Institute, Nanchang University, Nanchang 330047, China
Limin Pharmaceutical Factory, Livzon Group, Shaoguang 512028, China
Institute of Clinical Pharmacology, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China
Authors to whom correspondence should be addressed.
These authors contribute equally to this paper.
Academic Editor: Thomas Efferth
Received: 21 July 2016 / Revised: 11 August 2016 / Accepted: 15 August 2016 / Published: 19 August 2016
(This article belongs to the Collection Herbal Medicine Research)
PDF [2205 KB, uploaded 19 August 2016]


Quality control of Chinese medicine injections remains a challenge due to our poor knowledge of their complex chemical profile. This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis. A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis. The existence of polysaccharides was also examined on a gel permeation chromatography column. The method was well validated in terms of linearity, sensitivity, precision, accuracy and stability, and was successfully applied to analyze 13 SQI samples. The results demonstrate that up to 94.69% (w/w) of this injection product are quantitatively determined, in which small molecules and monosaccharide/sucrose account for 0.18%–0.21%, and 53.49%–58.2%, respectively. The quantitative information contributes to accumulating scientific evidence to better understand the therapy efficacy and safety of complex Chinese medicine injections. View Full-Text
Keywords: Shenqi Fuzheng injection; Chinese medicine formula; quantitative analysis; chemical profile Shenqi Fuzheng injection; Chinese medicine formula; quantitative analysis; chemical profile

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Chau, S.-L.; Huang, Z.-B.; Song, Y.-G.; Yue, R.-Q.; Ho, A.; Lin, C.-Z.; Huang, W.-H.; Han, Q.-B. Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies. Molecules 2016, 21, 1092.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top