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Molecules 2016, 21(8), 1021;

Siegesbeckia orientalis Extract Inhibits TGFβ1-Induced Migration and Invasion of Endometrial Cancer Cells

Department of Obstetrics & Gynecology, E-Da Hospital/I-Shou University, Kaohsiung 82445, Taiwan
Graduate Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung 84001, Taiwan
Department of Nutrition, I-Shou University, Kaohsiung 82445, Taiwan
Division of Cardiology, Department of Internal Medicine, E-Da Hospital/I-Shou University, Kaohsiung 82445, Taiwan
Author to whom correspondence should be addressed.
Academic Editors: Christopher W.K. Lam and Thomas Efferth
Received: 12 July 2016 / Revised: 1 August 2016 / Accepted: 3 August 2016 / Published: 5 August 2016
(This article belongs to the Collection Herbal Medicine Research)
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Type II endometrial carcinoma typically exhibits aggressive metastasis and results in a poor prognosis. Siegesbeckia orientalis Linne is a traditional Chinese medicinal herb with several medicinal benefits, including the cytotoxicity against various cancers. This study investigates the inhibitory effects of S. orientalis ethanol extract (SOE) on the migration and invasion of endometrial cancer cells, which were stimulated by transforming growth factor β (TGFβ). The inhibitory effects were evaluated by determining wound healing and performing the Boyden chamber assay. This study reveals that SOE can inhibit TGFβ1-induced cell wound healing, cell migration, and cell invasion in a dose-dependent manner in RL95-2 and HEC-1A endometrial cancer cells. SOE also reversed the TGFβ1-induced epithelial-mesenchymal transition, including the loss of the cell-cell junction and the lamellipodia-like structures. Western blot analysis revealed that SOE inhibited the phosphorylation of ERK1/2, JNK1/2, and Akt, as well as the expression of MMP-9, MMP-2, and u-PA in RL95-2 cells dose-dependently. The results of this investigation suggest that SOE is a potential anti-metastatic agent against human endometrial tumors. View Full-Text
Keywords: Siegesbeckia orientalis linne; endometrial cancer; anti-metastasis Siegesbeckia orientalis linne; endometrial cancer; anti-metastasis

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Chang, C.-C.; Ling, X.-H.; Hsu, H.-F.; Wu, J.-M.; Wang, C.-P.; Yang, J.-F.; Fang, L.-W.; Houng, J.-Y. Siegesbeckia orientalis Extract Inhibits TGFβ1-Induced Migration and Invasion of Endometrial Cancer Cells. Molecules 2016, 21, 1021.

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