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Open AccessArticle

Modulation of Neural Network Activity through Single Cell Ablation: An in Vitro Model of Minimally Invasive Neurosurgery

1
Neuroscience and Brain Technologies Department, Istituto Italiano di Tecnologia, Genoa 16163, Italy
2
Biocruces Health Research Institute, Cruces University Hospital, Barakaldo 48903, Spain
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
These authors are joint senior authors on this work.
Academic Editors: Tuhin Subhra Santra and Fan-Gang Tseng
Molecules 2016, 21(8), 1018; https://doi.org/10.3390/molecules21081018
Received: 10 June 2016 / Revised: 25 July 2016 / Accepted: 1 August 2016 / Published: 5 August 2016
(This article belongs to the Special Issue Micro/Nano Fluidics and Bio-MEMS)
The technological advancement of optical approaches, and the growth of their applications in neuroscience, has allowed investigations of the physio-pathology of neural networks at a single cell level. Therefore, better understanding the role of single neurons in the onset and progression of neurodegenerative conditions has resulted in a strong demand for surgical tools operating with single cell resolution. Optical systems already provide subcellular resolution to monitor and manipulate living tissues, and thus allow understanding the potentiality of surgery actuated at single cell level. In the present work, we report an in vitro experimental model of minimally invasive surgery applied on neuronal cultures expressing a genetically encoded calcium sensor. The experimental protocol entails the continuous monitoring of the network activity before and after the ablation of a single neuron, to provide a robust evaluation of the induced changes in the network activity. We report that in subpopulations of about 1000 neurons, even the ablation of a single unit produces a reduction of the overall network activity. The reported protocol represents a simple and cost effective model to study the efficacy of single-cell surgery, and it could represent a test-bed to study surgical procedures circumventing the abrupt and complete tissue removal in pathological conditions. View Full-Text
Keywords: long-term calcium imaging; GCaMP; single neuron ablation; single neuron firing rate; network firing rate long-term calcium imaging; GCaMP; single neuron ablation; single neuron firing rate; network firing rate
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MDPI and ACS Style

Soloperto, A.; Bisio, M.; Palazzolo, G.; Chiappalone, M.; Bonifazi, P.; Difato, F. Modulation of Neural Network Activity through Single Cell Ablation: An in Vitro Model of Minimally Invasive Neurosurgery. Molecules 2016, 21, 1018. https://doi.org/10.3390/molecules21081018

AMA Style

Soloperto A, Bisio M, Palazzolo G, Chiappalone M, Bonifazi P, Difato F. Modulation of Neural Network Activity through Single Cell Ablation: An in Vitro Model of Minimally Invasive Neurosurgery. Molecules. 2016; 21(8):1018. https://doi.org/10.3390/molecules21081018

Chicago/Turabian Style

Soloperto, Alessandro; Bisio, Marta; Palazzolo, Gemma; Chiappalone, Michela; Bonifazi, Paolo; Difato, Francesco. 2016. "Modulation of Neural Network Activity through Single Cell Ablation: An in Vitro Model of Minimally Invasive Neurosurgery" Molecules 21, no. 8: 1018. https://doi.org/10.3390/molecules21081018

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