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Construction of an Immobilized Thermophilic Esterase on Epoxy Support for Poly(ε-caprolactone) Synthesis

Department of Colorectal Surgery, the Second Hospital of Jilin University, Changchun 130041, China
Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China
Department of Polymer Science, College of Chemistry, Jilin University, Changchun 130012, China
Authors to whom correspondence should be addressed.
Academic Editor: Roberto Fernandez-Lafuente
Molecules 2016, 21(6), 796;
Received: 4 May 2016 / Revised: 14 June 2016 / Accepted: 16 June 2016 / Published: 18 June 2016
(This article belongs to the Special Issue Enzyme Immobilization 2016)
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Developing an efficient immobilized enzyme is of great significance for improving the operational stability of enzymes in poly(ε-caprolactone) synthesis. In this paper, a thermophilic esterase AFEST from the archaeon Archaeoglobus fulgidus was successfully immobilized on the epoxy support Sepabeads EC-EP via covalent attachment, and the immobilized enzyme was then employed as a biocatalyst for poly(ε-caprolactone) synthesis. The enzyme loading and recovered activity of immobilized enzyme was measured to be 72 mg/g and 10.4 U/mg using p-nitrophenyl caprylate as the substrate at 80 °C, respectively. Through the optimization of reaction conditions (enzyme concentration, temperature, reaction time and medium), poly(ε-caprolactone) was obtained with 100% monomer conversion and low number-average molecular weight (Mn < 1300 g/mol). Further, the immobilized enzyme exhibited excellent reusability, with monomer conversion values exceeding 75% during 15 batch reactions. Finally, poly(ε-caprolactone) was enzymatically synthesized with an isolated yield of 75% and Mn value of 3005 g/mol in a gram-scale reaction. View Full-Text
Keywords: thermophilic esterase; immobilization; epoxy support; ring-opening polymerization; poly(ε-caprolactone) thermophilic esterase; immobilization; epoxy support; ring-opening polymerization; poly(ε-caprolactone)

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Ren, H.; Xing, Z.; Yang, J.; Jiang, W.; Zhang, G.; Tang, J.; Li, Q. Construction of an Immobilized Thermophilic Esterase on Epoxy Support for Poly(ε-caprolactone) Synthesis. Molecules 2016, 21, 796.

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