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Molecules 2016, 21(6), 741;

Optimization of Preparation Conditions for Lysozyme Nanoliposomes Using Response Surface Methodology and Evaluation of Their Stability

National & Local United Engineering Lab of Quality Controlling Technology and Instrumentation for Marine Food, China Jiliang University, Hangzhou 310018, China
Department of Food Science, Ocean College, Zhejiang University of Technology, Hangzhou 310014, China
Inspection and Quarantine Center of Shandong Exit & Entry Inspection and Quarantine Burean, Qingdao 266002, China
Ningbo Inspection and Quarantine Institute of Science and Technology, Ningbo 315000, China
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Didier Astruc
Received: 31 March 2016 / Revised: 25 May 2016 / Accepted: 31 May 2016 / Published: 8 June 2016
(This article belongs to the Collection Nanomedicine)
Full-Text   |   PDF [3658 KB, uploaded 8 June 2016]   |  


The main purpose of this study was to optimize the preparation of lysozyme nanoliposomes using response surface methodology and measure their stability. The stabilities of lysozyme nanoliposomes in simulated gastrointestinal fluid (SGF), simulated intestinal fluid (SIF), as well as pH, temperature and sonication treatment time were evaluated. Reverse-phase evaporation method is an easy, speedy, and beneficial approach for nanoliposomes’ preparation and optimization. The optimal preparative conditions were as follows: phosphatidylcholine-to-cholesterol ratio of 3.86, lysozyme concentration of 1.96 mg/mL, magnetic stirring time of 40.61 min, and ultrasound time of 14.15 min. At the optimal point, encapsulation efficiency and particle size were found to be 75.36% ± 3.20% and 245.6 nm ± 5.2 nm, respectively. The lysozyme nanoliposomes demonstrated certain stability in SGF and SIF at a temperature of 37 °C for 4 h, and short sonication handling times were required to attain nano-scaled liposomes. Under conditions of high temperature, acidity and alkalinity, lysozyme nanoliposomes are unstable. View Full-Text
Keywords: lysozyme; nanoliposomes; response surface methodology; optimization; stability lysozyme; nanoliposomes; response surface methodology; optimization; stability

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Wu, Z.; Guan, R.; Lyu, F.; Liu, M.; Gao, J.; Cao, G. Optimization of Preparation Conditions for Lysozyme Nanoliposomes Using Response Surface Methodology and Evaluation of Their Stability. Molecules 2016, 21, 741.

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