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Molecules 2016, 21(5), 590;

The Application of Ultrasound in 3D Bio-Printing

Singapore Centre for 3D Printing (SC3DP), School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore 639798, Singapore
Academic Editors: Chee Kai Chua, Wai Yee Yeong and Jia An
Received: 27 March 2016 / Revised: 20 April 2016 / Accepted: 25 April 2016 / Published: 5 May 2016
(This article belongs to the Special Issue Biomaterials and Bioprinting)
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Three-dimensional (3D) bioprinting is an emerging and promising technology in tissue engineering to construct tissues and organs for implantation. Alignment of self-assembly cell spheroids that are used as bioink could be very accurate after droplet ejection from bioprinter. Complex and heterogeneous tissue structures could be built using rapid additive manufacture technology and multiple cell lines. Effective vascularization in the engineered tissue samples is critical in any clinical application. In this review paper, the current technologies and processing steps (such as printing, preparation of bioink, cross-linking, tissue fusion and maturation) in 3D bio-printing are introduced, and their specifications are compared with each other. In addition, the application of ultrasound in this novel field is also introduced. Cells experience acoustic radiation force in ultrasound standing wave field (USWF) and then accumulate at the pressure node at low acoustic pressure. Formation of cell spheroids by this method is within minutes with uniform size and homogeneous cell distribution. Neovessel formation from USWF-induced endothelial cell spheroids is significant. Low-intensity ultrasound could enhance the proliferation and differentiation of stem cells. Its use is at low cost and compatible with current bioreactor. In summary, ultrasound application in 3D bio-printing may solve some challenges and enhance the outcomes. View Full-Text
Keywords: three-dimensional bio-printing; bioink; cell spheroids; ultrasound standing wave field; bioreactor; low-intensity ultrasound three-dimensional bio-printing; bioink; cell spheroids; ultrasound standing wave field; bioreactor; low-intensity ultrasound

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Zhou, Y. The Application of Ultrasound in 3D Bio-Printing. Molecules 2016, 21, 590.

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