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Open AccessArticle

Validation and Application of an Ultra High-Performance Liquid Chromatography Tandem Mass Spectrometry Method for Yuanhuacine Determination in Rat Plasma after Pulmonary Administration: Pharmacokinetic Evaluation of a New Drug Delivery System

by Man Li 1,2,†, Xiao Liu 1,†, Hao Cai 1,†, Zhichun Shen 1, Liu Xu 1, Weidong Li 1, Li Wu 1, Jinao Duan 1,2 and Zhipeng Chen 1,2,*
1
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
2
Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing 210023, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Derek J. McPhee
Molecules 2016, 21(12), 1733; https://doi.org/10.3390/molecules21121733
Received: 14 November 2016 / Revised: 5 December 2016 / Accepted: 13 December 2016 / Published: 16 December 2016
(This article belongs to the Section Medicinal Chemistry)
Yuanhuacine was found to have significant inhibitory activity against A-549 human lung cancer cells. However, there would be serious adverse toxicity effects after systemic administration of yuanhuacine, such as by oral and intravenous ways. In order to achieve better curative effect and to alleviate the adverse toxicity effects, we tried to deliver yuanhuacine directly into the lungs. Ultra high-performance liquid chromatography tandem mass spectrometry (UHPLC–MS/MS) was used to detect the analyte and IS. After extraction (ether:dichloromethane = 8:1), the analyte and IS were separated on a Waters BEH-C18 column (100 mm × 2.1 mm, 1.7 μm) under a 5 min gradient elution using a mixture of acetonitrile and 0.1% formic acid aqueous solution as mobile phase at a flow rate of 0.3 mL/min. ESI positive mode was chosen for detection. The method was fully validated for its selectivity, accuracy, precision, stability, matrix effect, and extraction recovery. This new method for yuanhuacine concentration determination in rat plasma was reliable and could be applied for its preclinical and clinical monitoring purpose. View Full-Text
Keywords: yuanhuacine; pulmonary drug delivery; pharmacokinetics; ultra high-performance liquid chromatography tandem mass spectrometry yuanhuacine; pulmonary drug delivery; pharmacokinetics; ultra high-performance liquid chromatography tandem mass spectrometry
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MDPI and ACS Style

Li, M.; Liu, X.; Cai, H.; Shen, Z.; Xu, L.; Li, W.; Wu, L.; Duan, J.; Chen, Z. Validation and Application of an Ultra High-Performance Liquid Chromatography Tandem Mass Spectrometry Method for Yuanhuacine Determination in Rat Plasma after Pulmonary Administration: Pharmacokinetic Evaluation of a New Drug Delivery System. Molecules 2016, 21, 1733.

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