Next Article in Journal
Simultaneous Determination of Four Tanshinones by UPLC-TQ/MS and Their Pharmacokinetic Application after Administration of Single Ethanol Extract of Danshen Combined with Water Extract in Normal and Adenine-Induced Chronic Renal Failure Rats
Next Article in Special Issue
Anti-Bacterial and Anti-Fungal Activity of Xanthones Obtained via Semi-Synthetic Modification of α-Mangostin from Garcinia mangostana
Previous Article in Journal
Inhibition of Urease by Disulfiram, an FDA-Approved Thiol Reagent Used in Humans
Previous Article in Special Issue
Synthesis and Biological Evaluation of Benzimidazole Phenylhydrazone Derivatives as Antifungal Agents against Phytopathogenic Fungi
Open AccessArticle

Antifungal Activity of Oleuropein against Candida albicans—The In Vitro Study

1
Agency for Medicinal Products and Medical Devices of Croatia (HALMED), Ksaverska cesta 4, HR-10000 Zagreb, Croatia
2
Institute for Medical Research and Occupational Health, Ksaverska cesta 2, HR-10000 Zagreb, Croatia
3
Department of Microbiology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Schrottova 39, HR-10000 Zagreb, Croatia
*
Author to whom correspondence should be addressed.
Academic Editors: Daniela Barlocco and Fiorella Meneghetti
Molecules 2016, 21(12), 1631; https://doi.org/10.3390/molecules21121631
Received: 1 November 2016 / Revised: 21 November 2016 / Accepted: 24 November 2016 / Published: 28 November 2016
(This article belongs to the Special Issue Frontiers in Antimicrobial Drug Discovery and Design)
In the present study we investigated activity of oleuropein, a complex phenol present in large quantities in olive tree products, against opportunistic fungal pathogen Candida albicans. Oleuropein was found to have in vitro antifungal activity with a minimal inhibitory concentration (MIC) value of 12.5 mg·mL−1. Morphological changes in the nuclei after staining with fluorescent DNA-binding dyes revealed that apoptosis was a primary mode of cell death in the analyzed samples treated with subinhibitory concentrations of oleuropein. Our results suggest that this antifungal agent targets virulence factors essential for establishment of the fungal infection. We noticed that oleuropein modulates morphogenetic conversion and inhibits filamentation of C. albicans. The hydrophobicity assay showed that oleuropein in sub-MIC values has significantly decreased, in both aerobic and anaerobic conditions, the cellular surface hydrophobicity (CSH) of C. albicans, a factor associated with adhesion to epithelial cells. It was also demonstrated that the tested compound inhibits the activity of SAPs, cellular enzymes secreted by C. albicans, which are reported to be related to the pathogenicity of the fungi. Additionally, we detected that oleuropein causes a reduction in total sterol content in the membrane of C. albicans cells, which might be involved in the mechanism of its antifungal activity. View Full-Text
Keywords: oleuropein; antifungal activity; Candida albicans; virulence factors oleuropein; antifungal activity; Candida albicans; virulence factors
Show Figures

Figure 1

MDPI and ACS Style

Zorić, N.; Kopjar, N.; Bobnjarić, I.; Horvat, I.; Tomić, S.; Kosalec, I. Antifungal Activity of Oleuropein against Candida albicans—The In Vitro Study. Molecules 2016, 21, 1631.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop