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Analysis of Flavonoids in Rhamnus davurica and Its Antiproliferative Activities

1,2, 1,2, 1,2 and 1,3,*
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China
Graduate University of Chinese Academy of Sciences, Beijing 100049, China
Sino-Africa Joint Research Center, Chinese Academy of Sciences, Wuhan 430074, China
Author to whom correspondence should be addressed.
Academic Editor: Celestino Santos-Buelga
Molecules 2016, 21(10), 1275;
Received: 28 July 2016 / Revised: 18 September 2016 / Accepted: 21 September 2016 / Published: 23 September 2016
(This article belongs to the Special Issue Flavonoids: From Structure to Health Issues)
PDF [1748 KB, uploaded 23 September 2016]


Rhamnus davurica Pall. (R. davurica) has been used as a traditional medicinal herb for many years in China and abroad. It has been well documented as a rich source of flavonoids with diversified structures, which in turn results in far-ranging biological activities, such as anti-inflammation, anticancer, antibacterial and antioxidant activities. In order to further correlate their anticancer potentials with the phytochemical components, the fingerprint profile of R. davurica herb from Dongbei was firstly investigated using HPLC-ESI-MS/MS. Thirty two peaks were detected and identified, 14 of which were found in R. davurica for the first time in this work. Furthermore, a total of 23 peaks were resolved as flavonoids, which are the major components found in R. davurica. Meanwhile, the antiproliferative activities against human cancer cells of HT-29 and SGC-7901 in vitro exhibited distinct inhibitory effects with IC50 values at 24.96 ± 0.74 and 89.53 ± 4.11 μg/mL, respectively. Finally, the general toxicity against L-O2 cells displayed a much higher IC50 at 229.19 ± 8.52 μg/mL, which suggested very low or no toxicity on hepatic cell viability. The current study revealed for the first time the correlations between the flavonoids of R. davurica with their antiproliferative activities, which indicated that the fingerprint profile of flavonoids and their anticancer activities could provide valuable information on the quality control for herbal medicines and their derived natural remedies from this valuable medicinal plant. View Full-Text
Keywords: Rhamnus davurica; fingerprint profile; flavonoids; antiproliferative activities; HPLC-ESI-MS/MS Rhamnus davurica; fingerprint profile; flavonoids; antiproliferative activities; HPLC-ESI-MS/MS

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Chen, G.; Li, X.; Saleri, F.; Guo, M. Analysis of Flavonoids in Rhamnus davurica and Its Antiproliferative Activities. Molecules 2016, 21, 1275.

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