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Molecules 2016, 21(1), 15;

Flavones Isolated from Scutellariae radix Suppress Propionibacterium Acnes-Induced Cytokine Production In Vitro and In Vivo

Department of Human Development and Family Studies, National Taiwan Normal University, Taipei 106, Taiwan
National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan
Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944, Taiwan
Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404, Taiwan
Department of Biotechnology, Asia University, Taichung 413, Taiwan
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 2 November 2015 / Revised: 3 December 2015 / Accepted: 14 December 2015 / Published: 24 December 2015
(This article belongs to the Section Medicinal Chemistry)
Full-Text   |   PDF [1942 KB, uploaded 24 December 2015]   |  


Scutellariae radix, the root of Scutellaria baicalensis, has long been applied in traditional formulations and modern herbal medications. Propionibacterium acnes (P. acnes) in follicles can trigger inflammation and lead to the symptom of inflammatory acnes vulgaris. This study was aimed at evaluating the effect of Scutellariae radix extract and purified components isolated from it on inflammation induced by P. acnes in vitro and in vivo. The results showed the ethyl acetate (EA) soluble fraction from the partition of crude ethanolic extract from Scutellariae radix inhibited P. acnes-induced interleukin IL-8 and IL-1β production in human monocytic THP-1 cells. Seven flavones were isolated from the EA fraction by repeated chromatographies, and identified as 5,7-dihydroxy-6-methoxyflavone (FL1, oroxylin), 5,7-dihydroxy-8-methoxyflavone (FL2, wogonin), 5-hydroxy-7,8-dimethoxyflavone (FL3, 7-O-methylwogonin), 5,6′-dihydroxy-6,7,8,2′-tetramethoxy flavone (FL4, skullcapflavone II), 5,7,4′-trihydroxy-8-methoxyflavone (FL5), 5,2′,6′-trihydroxy-7,8-dimethoxyflavone (FL6, viscidulin II), and 5,7,2′,5′-tetrahydroxy-8,6′-dimethoxyflavone (FL7, ganhuangenin). They all significantly suppressed P. acnes-induced IL-8 and IL-1β production in THP-1 cells, and FL2 exerted the strongest effect with half maximal inhibition (IC50) values of 8.7 and 4.9 μM, respectively. Concomitant intradermal injection of each of the seven flavones (20 μg) with P. acnes effectively attenuated P. acnes-induced ear swelling, and decreased the production of IL-6 and tumor necrosis factor-α in ear homogenates. Our results suggested that all the seven flavones can be potential therapeutic agents against P. acnes-induced skin inflammation. View Full-Text
Keywords: Chinese herb; Scutellariae radix ; flavone; anti-inflammation; Propionibacterium acnes Chinese herb; Scutellariae radix ; flavone; anti-inflammation; Propionibacterium acnes

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Tsai, P.-J.; Huang, W.-C.; Hsieh, M.-C.; Sung, P.-J.; Kuo, Y.-H.; Wu, W.-H. Flavones Isolated from Scutellariae radix Suppress Propionibacterium Acnes-Induced Cytokine Production In Vitro and In Vivo. Molecules 2016, 21, 15.

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