Rapid Estimation of Tocopherol Content in Linseed and Sunflower Oils-Reactivity and Assay
AbstractThe reactivity of tocopherols with 2,2-diphenyl-1-picrylhydrazyl (DPPH) was studied in model systems in order to establish a method for quantifying vitamin E in plant oils. The method was optimized with respect to solvent composition of the assay medium, which has a large influence on the course of reaction of tocopherols with DPPH. The rate of reaction of α-tocopherol with DPPH is higher than that of γ-tocopherol in both protic and aprotic solvents. In ethyl acetate, routinely applied for the analysis of antioxidant potential (AOP) of plant oils, reactions of tocopherols with DPPH are slower and concentration of tocopherols in the assay has a large influence on their molar reactivity. In 2-propanol, however, two electrons are exchanged for both α- and γ-tocopherols, independent of their concentration. 2-propanol is not toxic and is fully compatible with polypropylene labware. The chromatographically determined content of tocopherols and their molar reactivity in the DPPH assay reveal that only tocopherols contribute to the AOP of sunflower oil, whereas the contribution of tocopherols to the AOP of linseed oil is 75%. The DPPH assay in 2-propanol can be applied for rapid and cheap estimation of vitamin E content in plant oils where tocopherols are major antioxidants. View Full-Text
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Prevc, T.; Levart, A.; Cigić, I.K.; Salobir, J.; Ulrih, N.P.; Cigić, B. Rapid Estimation of Tocopherol Content in Linseed and Sunflower Oils-Reactivity and Assay. Molecules 2015, 20, 14777-14790.
Prevc T, Levart A, Cigić IK, Salobir J, Ulrih NP, Cigić B. Rapid Estimation of Tocopherol Content in Linseed and Sunflower Oils-Reactivity and Assay. Molecules. 2015; 20(8):14777-14790.Chicago/Turabian Style
Prevc, Tjaša; Levart, Alenka; Cigić, Irena K.; Salobir, Janez; Ulrih, Nataša P.; Cigić, Blaž. 2015. "Rapid Estimation of Tocopherol Content in Linseed and Sunflower Oils-Reactivity and Assay." Molecules 20, no. 8: 14777-14790.