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Article

Synthesis of Bicyclic Isoxazoles and Isoxazolines via Intramolecular Nitrile Oxide Cycloaddition

by
Wen-Chang Chen
,
Veerababurao Kavala
,
Yu-Hsuan Shih
,
Yu-Hsuan Wang
,
Chun-Wei Kuo
,
Tang-Hao Yang
,
Chia-Yu Huang
,
Hao-Hsiang Chiu
and
Ching-Fa Yao
*
Department of Chemistry, National Taiwan Normal University, 88, Sec. 4, Ting-Chow Road, Taipei 116, Taiwan
*
Author to whom correspondence should be addressed.
Molecules 2015, 20(6), 10910-10927; https://doi.org/10.3390/molecules200610910
Submission received: 30 April 2015 / Revised: 1 June 2015 / Accepted: 4 June 2015 / Published: 12 June 2015
(This article belongs to the Section Organic Chemistry)
Browse Figures
Versions Notes

Abstract

:
An efficient and straight forward procedure for the syntheses of bicyclic isoxazole/isoxazoline derivatives from the corresponding dimethyl-2-(2-nitro-1-aryl/alkyl)-2-(prop-2-yn-1yl)malonates or dimethyl 2-allyl-2-(2-nitro-1-aryl/alkyl ethyl)malonate is described. High yields and simple operations are important features of this methodology.

Graphical Abstract

1. Introduction

Isoxazole and isoxazoline derivatives are important scaffolds found in many naturally occurring and biologically active compounds [1,2,3,4,5]. They are considered to be important precursors for the synthesis of β-hydroxyketones or β-aminoalcohols, α,β-unsaturated ketones, and many other valuable compounds [6,7,8,9,10]. Isoxazole or isoxazoline motifs fused with carbocycles or heterocycles are known to possess a variety of bioactivities and are also useful surrogates for the generation of various other bioactive compounds [11,12,13,14,15].
Isoxazole and isoxazoline derivatives are typically synthesized through in situ formation of a nitrile oxide followed by an intramolecular dipolar cycloaddition [16,17,18,19]. The most common methods for generating nitrile oxides are the dehydrohalogenation of hydroximoyl chlorides [20,21,22,23,24], the oxidation of aldoximes [25,26,27,28,29,30,31,32], and the dehydration of nitroalkanes [33,34,35,36]. Although a wide variety of procedures are available for the synthesis of diverse isoxazole and isoxazoline derivatives, few of them address the preparation of carbocyclic fused isoxazole/isoxazoline derivatives [37,38,39,40,41,42]. The dehydration of the nitroalkanes followed by intramolecular dipolar cycloaddition is the most popular method for the construction of carbocycle fused isoxazole/isoxazoline derivatives. The literature procedures for the generation of carbocycle fused isoxazole/isoxazoline from various nitroalkane derivatives [43,44,45,46,47] involve the use of phenyl isocyanate and triethylamine, treatment of the nitronate with di-tert-butyl dicarbonate in the presence of catalytic amounts of dimethylaminopyridine (DMAP), the use of chlorformates in the presence trimethylamine and trimethylsilyl chloride (TMSCl) in the presence of trimethylamine. We also reported on a different procedure for the construction of bicyclicisoxazole/isoxazoline derivatives by using trichlorotriazine (TCT), chloroformate and phenyl isocyante as dehydrating agents. Although a few methods for the synthesis of carbocycle fused isoxazole/isoxazoline derivatives are efficient, some of the available methods suffer from drawbacks such as low yields and selectivity and long reaction times. Hence, a simple and straightforward method for generating such derivatives in high yields and in better selectivity would be highly desirable. It is well known that the Yamaguchi reagent (2,4,6-trichlorobenzoyl chloride) is a dehydrating agent used in the construction of macrolides and highly functionalized esters [48,49,50]. However, to our knowledge this reagent has not been utilized for the dehydration of nitroalkanes. Herein, we wish to report on the synthesis of bicyclicisoxazole/isoxazoline derivatives from the corresponding dimethyl 2-propargyl-2-(2-nitro-1-aryl/alkylethyl)malonate/dimethyl 2-allyl-2-(2-nitro-1-aryl/alkyl ethyl)malonates using the Yamaguchi reagent as a dehydrating agent.

2. Results and Discussion

We first synthesized the starting materials, including the dimethyl 2-propargyl-2-(2-nitro-1-aryl/alkylethyl)malonates 1a–15a or dimethyl 2-allyl-2-(2-nitro-1-aryl/alkyl ethyl)malonates 1c–6c from the corresponding nitroalkenes and propargylmalonate/allylmalonate derivatives in the presence of potassium tertiary butoxide (t-BuOK) and lithium bis(trimethylsilyl)amide (LHMDS) [34,43]. We then initiated our studies of the reaction by using dimethyl-2-(2-nitro-1-phenylethyl)-2-(prop-2-yn-1yl)malonate (1a) as the substrate.
To start the reaction, 1a was treated with potassium tert-butoxide (2.5 equiv.) and the Yamaguchi reagent (2,4,6-trichlorobenzoyl chloride) in dichloromethane at −78 °C. The corresponding bicyclic isoxazole product was produced in 65% yield after 24 h. Encouraged by this initial result, we next focused our attention on optimizing the reaction conditions for the formation the bicyclic isoxazole product. To find a superior base for this transformation the reaction, various bases, including trimethylamine (Et3N), 1,8-diazabicycloundec-7-ene (DBU), and dimethylaminopyridine (DMAP) were screened. Among them, DBU was found to be the most suitable one. As the Yamaguchi reagent functions as a dehydrating agent in this reaction, we were curious to examine whether other benzoyl chlorides could be used for this purpose. To check this, we reacted 1a with various benzoyl chlorides including unsubstituted, 2-chloro-, and 3,4-dichlorobenzoyl chlorides in the presence of DBU. All of these reactions resulted in the production of the desired product in moderate to good yields. However, the yield of the desired product was higher when the Yamaguchi reagent was used as the dehydrating agent. Although the yield of the product was satisfactory, the reaction time was longer when a base (2.5 equiv.) and a dehydrating agent (3 equiv.) was used.
On the other hand, we observed that the efficiency of the nitroalkane to nitrile oxide dehydration reaction was enhanced in the presence a catalytic amount of Lewis acid [43] and conducting the reaction in the presence of a Lewis acid produced the corresponding product in a very short time. Hence, we tested various Lewis acid catalysts, including SnCl4, ZnCl2 and ZrCl4. Among the Lewis acids tested, ZrCl4 was found to the best choice. Further, to determine the optimum amount of base required for this reaction, we carried out the reaction using 2 equiv., 1.5 equiv., and 1.0 equiv. of DBU. The reaction was the most efficient when more than 1.5 equivalent of base was used. Moreover, the reaction resulted in excellent yields and the reaction time was shorter when a base (1.5 equiv.) and 1.5 equiv. of the Yamaguchi reagent was used. To determine the effect of temperature, we conducted the reactions at 0 °C and at room temperature. The reaction furnished the desired bicyclic isoxazole derivative in moderate yield when it was conducted at 0 °C, whereas a poor yield of the bicyclic isoxazole was obtained when the reaction was carried out at room temperature. Thus, the optimum reaction conditions for the formation of the bicyclic isoxazole were determined to be the use of 1.5 equiv. DBU, 1.5 equiv. of the Yamaguchi reagent and 10 mol % ZrCl4 as the catalyst in DCM at −78 °C. These optimization studies are summarized in Table 1.
Table
Table 1. Optimization studies.
Molecules 20 10910 i001
Table 1. Optimization studies.
Molecules 20 10910 i001
EntryBase (equiv.)Dehydrating Agent (equiv.)Lewis AcidTemp. (°C)Time (h)Yield a, b
1K t-BuO(2.5)2,4,6-Trichlorobenzoyl chloride(3)—‒782465
2TEA(2.5)2,4,6-Trichlorobenzoyl chloride(3)—‒782450
3DMAP(2.5)2,4,6-Trichlorobenzoyl chloride(3)—‒782467
4DBU(2.5)2,4,6-Trichlorobenzoyl chloride(3)—‒782473
5DBU(2.5)Benzoyl chloride(3)—‒784862
6DBU(2.5)2-Chlorobenzoyl; chloride(3)—‒783565
7DBU(2.5)3,4-Dichlorobenzoyl chloride(3)—‒782465
8DBU(2.5)2,4,6-Trichlorobenzoyl chloride(1.5)ZnCl2‒78382
9DBU(2.5)2,4,6-Trichlorobenzoyl chloride(1.5)ZnCl4‒78487
10DBU(2.5)2,4,6-Trichlorobenzoyl chloride(1.5)SnCl4‒78560
11DBU(2.0)2,4,6-Trichlorobenzoyl chloride(1.5)ZnCl4‒78490
12DBU(1.5)2,4,6-Trichlorobenzoyl chloride(1.5)ZnCl4‒78495
13DBU(1.0)2,4,6-Trichlorobenzoyl chloride(1.5)ZnCl4‒78785
14DBU(1.5)2,4,6-Trichlorobenzoyl chloride(1.5)ZnCl40240
15DBU(1.5)2,4,6-Trichlorobenzoyl chloride(1.5)ZnCl425220
a NMR yields. b Reactions carried out in 0.5 mmol scale.
After determining the optimum conditions for the formation of bicyclic isoxazoles, we then utilized these conditions for investigating the scope and limitations of the methodology with various dimethyl-2-(2-nitro-1-aryl/alkyl)-2-(prop-2-yn-1yl)malonates derived from the corresponding nitroalkenes and dimethylproparylmalonate (Table 2).
Table
Table 2. Synthesis of bicyclic isoxazole derivatives.
Molecules 20 10910 i002
Table 2. Synthesis of bicyclic isoxazole derivatives.
Molecules 20 10910 i002
EntrySubstrateProductTime (h)Yield% a,b
1 Molecules 20 10910 i003
1a		 Molecules 20 10910 i004
2a		495
2 Molecules 20 10910 i005
1b		 Molecules 20 10910 i006
2b		286
3 Molecules 20 10910 i007
1c		 Molecules 20 10910 i008
2c		389
4 Molecules 20 10910 i009
1d		 Molecules 20 10910 i010
2d		392
5 Molecules 20 10910 i011
1e		 Molecules 20 10910 i012
2e		590
6 Molecules 20 10910 i013
1f		 Molecules 20 10910 i014
2f		587
7 Molecules 20 10910 i015
1g		 Molecules 20 10910 i016
2g		384
8 Molecules 20 10910 i017
1h		 Molecules 20 10910 i018
2h		386
9 Molecules 20 10910 i019
1i		 Molecules 20 10910 i020
2i		384
10 Molecules 20 10910 i021
1j		 Molecules 20 10910 i022
2j		1579
11 Molecules 20 10910 i023
1k		 Molecules 20 10910 i024
2k		792
12 Molecules 20 10910 i025
1l		 Molecules 20 10910 i026
2l		1280
13 Molecules 20 10910 i027
1m		 Molecules 20 10910 i028
2m		587
a Isolated yields. b Reactions performed in 0.5 mmol scale.
Under the optimized conditions, the substrate containing an unsubstituted phenyl group (compound 1a) gave the corresponding bicyclic isoxazole derivative in excellent yield. The reactions of substrates possessing an electron withdrawing group such as a bromo group (compound 1b) and substrates containing an electron donating group such as methyl (compound 1c) and methoxy (compound 1d) groups at the ortho position of the phenyl group reacted smoothly to yield the corresponding bicyclic isoxazole derivatives in excellent yields. On the other hand, substrates containing an electron withdrawing group at the para position of the phenyl group such as 1e and 1f, provided the corresponding bicycloisoxazole derivatives 2e and 2f, respectively, in excellent yields. In addition, substrates possessing electron donating groups (OMe and Me) and an electron neutral group (SMe) at the para position of the phenyl group reacted with equal ease to provide the desired products 2g, 2h and 2i in good yields. Further, substrates containing thiophene (compound 1j), and methyleneoxy groups (compound 1k) reacted smoothly and provided the corresponding bicyclicisoxazole derivatives 2j and 2k, respectively, in good to excellent yields. Substrates containing a naphthalene moiety (compound 2l) and an alkyl group (compound 2m) also produced the corresponding products in high yields under the present reaction conditions.
To extend the scope of this reaction, we examined the reactions of dimethyl 2-(2-nitro-1-phenylethyl)-2-(3-phenylprop-2-yn-1-yl)malonate (1n) and tetramethyl 2,2′-(1,4-phenylenebis(2-nitroethane-1,1-diyl))bis(2-(prop-2-yn-1-yl)malonate) (1o). To our delight, both reactions provided the corresponding bicyclic isoxazole derivatives (2n and 2o) in good yields (Scheme 1).
Molecules 20 10910 g001 550
Scheme 1. Synthesis of bicyclic isoxazole derivatives.
Scheme 1. Synthesis of bicyclic isoxazole derivatives.
Molecules 20 10910 g001
We also investigated the reactions of 2-allyl-2-(2-nitro-1-aryl/alkyl ethyl)malonate derivatives under the present reaction conditions and the results are listed in Table 3. As shown in Table 3, the reactions of substrates containing both electron withdrawing as well as electron donating groups exhibited a similar reactivity under the reaction conditions. However, when substrates containing thienyl and naphthyl moieties were used a slightly longer reaction time was needed to produce good yield of the corresponding bicyclic isoxazoline derivatives (Table 3).
It is important to note that the reactions of all 2-allyl-2-(2-nitro-1-aryl/alkylethyl)malonate derivatives resulted in the production of mixture of diastereomers and among the diastereomers, the cis isomer was the major component and the trans isomer was the minor one. The NMR signal for the methine proton at the bridgehead carbon in the trans isomer appears at 4.62 ppm, compared to 3.82 ppm for the cis isomer, which permits trans and cis isomers to be identified. The diastereoslectivity (cis:trans) ranged from 5.6:1 to 3.6:1. The diasteroslectivity for this reaction slightly better than in our previous report [43]. These results were consistent with the our previous results as well as other literature reports [43,51,52].
Table
Table 3. Synthesis of bicyclic isoxazoline derivatives.
Molecules 20 10910 i029
Table 3. Synthesis of bicyclic isoxazoline derivatives.
Molecules 20 10910 i029
EntrySubstrateProductTime (h)Yield% a,bcis:trans c
1 Molecules 20 10910 i030
3a		 Molecules 20 10910 i031
4a		3885.3:1
2 Molecules 20 10910 i032
3b		 Molecules 20 10910 i033
4b		4834.6:1
3 Molecules 20 10910 i034
3c		 Molecules 20 10910 i035
4c		5813.6:1
4 Molecules 20 10910 i036
3d		 Molecules 20 10910 i037
4d		5855.4:1
5 Molecules 20 10910 i038
3e		 Molecules 20 10910 i039
4e		12784.8:1
6 Molecules 20 10910 i040
3f		 Molecules 20 10910 i041
4f		7845.6:1
a Isolated yields. b Reactions performed in 0.5 mmol scale. c cis:trans isomers were determined from 1H-NMR.
Based on our experience with nitroalkanes and the literature reports related to the Yamaguchi reagent, the formation of bicyclic isoxazole/isoxazolines from dimethyl 2-propargyl-2-(2-nitro-1-aryl/alkylethyl)malonates or dimethyl 2-allyl-2-(2-nitro-1-aryl/alkylethyl)malonates with a base and the Yamaguchi reagent in the presence of an acidic catalyst could be explained by two mechanistic pathways shown in Scheme 2. The reaction of the nitroalkane in the presence a base could result in the production of a nitronate ion, which could be further converted into a benzoylnitronate [A] by reaction with the Yamaguchi reagent and zirconium chloride. This benzoylnitronate [A] could then be converted into the corresponding nitrile oxide [B] intermediate in the presence of zirconium chloride, which would further undergo intramolecular nitrile oxide cycloaddition (INOC) to afford the desired carbocycle fused isoxazole/isoxazoline (Scheme 2, Route B). Another possibility is that the benzoylnitronate might act as a 1,3-dipole [53] to undergo INOC to obtain intermediate [C], which upon losing trichlorobenzoic acid, would produce the desired bicyclic compound (Scheme 2, Route A).
Molecules 20 10910 g002 550
Scheme 2. Plausible mechanistic pathways.
Scheme 2. Plausible mechanistic pathways.
Molecules 20 10910 g002

3. Experimental Section

3.1. General Information

All chemicals were purchased from various commercial sources and used directly without further purification. Analytical thin-layer chromatography was performed using E. Merck (New York, NY, USA) silica gel 60F glass plates and E. Merck silica gel 60 (230–400 mesh) was used in flash chromatography separations. MS were measured by a JMS-HX110 spectrometer (JEOL, Hsinchu, Japan). HRMS spectra were recorded using ESI-TOF or EI+ mode or FAB+. 1H- (400 MHz) and 13C-NMR (100 MHz) spectra were recorded with an Advance EX 400 MHz spectrometer (Bruker, San Francisco, CA, USA). Chemical shifts are reported in parts per million (δ) using TMS as an internal standard and coupling constant were expressed in hertz. IR spectra were performed on a 100 series FT-IR instrument (Perkin Elmer, Waltham, MA, USA). Melting points were recorded using an capillary melting point apparatus (Electrothermal, Staffordshire, UK) and are uncorrected. All substrates were prepared using literature procedures.

3.2. General Procedure for the Preparation of 1a–1o

Potassium tert-butoxide (3 mmol) was added to a solution of dimethyl propargylmalonate (2.4 mmol) in anhydrous THF (15 mL) at room temperature under nitrogen atmosphere. The reaction mixture was then cooled to −78 °C and a solution of the nitroalkene (2 mmol) in anhydrous THF (5 mL) was added dropwise over a period of five minutes. The reaction mixture was then stirred at −78 °C until the reaction was complete, which was monitored by TLC. A saturated aqueous NH4Cl was then added and the resulting mixture extracted with ethyl acetate (3 × 10 mL). The combined organic phases were dried over MgSO4 and the solvent removed in vacuo. The residue was purified by flash column chromatography over silica (ethyl acetate and hexane).
Dimethyl 2-(1-(2-bromophenyl)-2-nitroethyl)-2-(prop-2-yn-1-yl)malonate (1b): Colorless solid; Yield: 67% (5 h); m.p. 100–101 °C; 1H-NMR (CDCl3) δ 7.60 (d, J = 8.0 Hz, 1H), 7.30 (t, J = 7.6 Hz, 1H), 7.20–7.14 (m, 2H), 5.22 (d, J = 8.0 Hz, 1H), 5.16–5.03 (m, 2H), 3.82 (s, 3H), 3.80 (s, 3H), 2.76 (m, 2H), 2.15 (s, 1H); 13C-NMR (CDCl3) δ 169.2, 169.1, 134.8, 134.2, 130.1, 128.4, 128.2, 127.3, 78.8, 78.0, 72.8, 60.8, 53.6, 53.3, 44.8, 23.8; HRMS (EI) m/z calc. for C16H16NO679Br (M+) 397.0161, found 397.0156.
Dimethyl 2-(2-nitro-1-(o-tolyl)ethyl)-2-(prop-2-yn-1-yl)malonate (1c): Colorless solid; Yield: 75% (4 h); m.p. 98–99 °C. 1H-NMR (CDCl3) δ 7.17–7.12 (m, 3H), 6.92–6.90 (m, 1H), 5.32 (dd, J = 6.7, 3.1 Hz, 1H), 4.96 (dd, J = 11.6 Hz, 6.6 Hz, 1H), 4.81 (dd, J = 5.6 Hz,, 2.9 Hz, 1H), 3.83 (s, 3H), 3.78 (s, 3H), 2.85 (dd, J = 8.7 Hz, 2.8 Hz, 1H), 2.51 (s, 3H), 2.41 (dd, J = 8.7 Hz, 2.6 Hz, 1H), 2.15 (t, J = 2.7 Hz, 1H); 13C-NMR (CDCl3) δ 169.5, 169.4, 139.2, 133.7, 131.7, 128.5, 126.6, 126.4, 79.2, 79.0, 73.2, 60.8, 53.4, 53.3, 40.8, 23.7, 20.1; HRMS (EI) m/z calc. for C17H19NO6 (M+) 372.1059, found 372.1069.
Dimethyl 2-(2-nitro-1-(2-methoxyphenyl)ethyl)-2-(prop-2-yn-1-yl)malonate (1d): Colorless solid; Yield: 72% (4 h); m.p. 141–142 °C. 1H-NMR (CDCl3) δ 7.26–7.22 (m, 2H), 6.88–6.81 (m, 2H), 5.17 (d, J = 5.8 Hz, 2H), 4.65 (s, 1H), 3.77 (s, 3H), 3.75 (s, 3H), 3.71 (s, 3H), 2.80 (d, J = 17.1 Hz, 1H), 2.30 (d, J = 16.3 Hz, 1H), 2.11 (s, 1H); 13C-NMR (CDCl3) δ 169.5, 169.1, 157.6, 133.0, 130.0, 122.3, 120.9, 111.2, 78.6, 76.4, 72.6, 59.0, 55.3, 53.2, 52.7, 44.0, 24.3.
Dimethyl 2-(1-(4-fluorophenyl)-2-nitroethyl)-2-(prop-2-yn-1-yl)malonate (1e): Colorless solid; Yield: 84% (3 h); m.p. 89–90 °C. 1H-NMR (CDCl3) δ 7.18 (dd, J = 8.4, 5.3 Hz, 2H), 7.00 (t, J = 8.5 Hz, 2H), 5.28 (dd, J = 13.6, 3.0 Hz, 1H), 4.96 (dd, J = 13.5, 11.6 Hz, 1H), 4.50 (dd, J = 11.7, 2.9 Hz, 1H), 3.80 (s, 3H), 3.78 (s, 3H), 2.79 (dd, J =17.5 Hz, 2.5 Hz, 1H), 2.37 (dd, J =17.5 Hz, 2.5 Hz, 1H), 2.23 (t, J = 2.3 Hz, 1H); 13C-NMR (CDCl3) δ 169.1, 169.0, 164.0 (d, JC-F = 245.0), 130.7 (d, JC-F = 8.0), 130.6 (d, JC-F = 4.0), 116.3 (d, JC-F = 21.0), 78.0, 76.9, 73.8, 59.7, 53.6, 53.4, 44.9, 24.1; HRMS (EI) m/z calc. for C16H16NO6F (M+) 337.0963, found 337.0969.
Dimethyl 2-(1-(4-chlorophenyl)-2-nitroethyl)-2-(prop-2-yn-1-yl)malonate (1f): Colorless solid; Yield: 98% (5 h); m.p. 123–124 °C; 1H-NMR (CDCl3) δ 7.30 (d, J = 8.3 Hz, 2H), 7.15 (d, J = 8.3 Hz, 2H), 5.30 (dd, J = 13.8, 2.9 Hz, 1H), 4.97 (dd, J = 13.8, 11.7 Hz, 1H), 4.51 (dd, J = 11.7, 2.9 Hz, 1H), 3.81 (s, 3H), 3.79 (s, 3H), 2.81 (dd, J =17.5 Hz, 2.2 Hz, 1H), 2.37 (dd, J =17.5 Hz, 2.2 Hz, 1H), 2.25 (s, 1H); 13C-NMR (CDCl3) δ 168.9, 168.8, 134.8, 133.2, 130.2, 129.3, 77.9, 77.5, 73.9, 59.5, 53.6, 53.4, 44.9, 24.1; HRMS (EI) m/z calc. for C16H16NO635Cl (M+) 353.0666, found 353.0661.
Dimethyl 2-(1-(4-methoxyphenyl)-2-nitroethyl)-2-(prop-2-yn-1-yl)malonate (1g): Colorless solid; Yield: 81% (2 h); m.p. 111–112 °C; 1H-NMR (CDCl3) δ 7.11 (d, J = 8.5 Hz, 2H), 6.84 (d, J = 8.5 Hz, 2H), 5.27 (dd, J = 13.5, 2.9 Hz, 1H), 4.98 (dd, J = 13.5, 11.4 Hz, 1H), 4.46 (dd, J = 11.4, 2.9 Hz, 1H), 3.81 (s, 3H), 3.79 (s, 3H), 3.77 (s, 3H), 2.79 (dd, J =17.4 Hz, 2.2 Hz, 1H), 2.42 (dd, J =17.4 Hz, 2.2 Hz, 1H), 2.22 (s, 1H); 13C-NMR (CDCl3) δ 169.2, 169.1, 159.9, 130.0, 126.4, 114.5, 78.3, 77.9, 73.5, 59.9, 55.4, 53.4, 53.3, 45.0, 24.1; HRMS (EI) m/z calc. for C17H19NO7 (M+) 349.1162, found 349.1156.
Dimethyl 2-(2-nitro-1-(p-tolyl)ethyl)-2-(prop-2-yn-1-yl)malonate (1h): Colorless solid; Yield: 92% (2 h); m.p. 91–92 °C; 1H-NMR (CDCl3) δ 7.15 (d, J = 8.0 Hz, 2H), 7.05 (d, J = 8.0 Hz, 2H), 5.28 (dd, J = 13.6, 3.0 Hz, 1H), 5.00 (dd, J = 13.6, 11.6 Hz, 1H), 4.47 (dd, J = 11.6, 2.9 Hz, 1H), 3.81 (s, 3H), 3.78 (s, 3H), 2.78 (dd, J =17.4 Hz, 2.4 Hz, 1H), 2.41 (dd, J =17.4 Hz, 2.4 Hz, 1H), 2.30 (s, 3H), 2.23 (s, 1H); 13C-NMR (CDCl3) δ 169.2, 169.1, 138.7, 131.5, 129.8, 128.6, 78.3, 77.8, 73.5, 59.7, 53.4, 53.3, 45.2, 24.1, 21.2; HRMS (EI) m/z calc. for C17H19NO6 (M+) 333.1212, found 333.1207.
Dimethyl 2-(2-nitro-1-(4-methylthiophenyl)ethyl)-2-(prop-2-yn-1-yl)malonate (1i): Yellow oil; Yield: 88% (2 h); 1H-NMR (CDCl3) δ 7.16 (d, J = 8.4 Hz, 2H), 7.09 (d, J = 8.4 Hz, 2H), 5.26 (dd, J = 13.7, 3.1 Hz, 1H), 4.97 (dd, J = 13.7, 11.4 Hz, 1H), 4.46 (dd, J = 11.4, 3.1 Hz, 1H), 3.79 (s, 3H), 3.77 (s, 3H), 2.78 (dd, J =17.4 Hz, 2.6 Hz, 1H), 2.43 (s, 3H), 2.39 (dd, J =17.4, 2.4 Hz, 1H), 2.22 (t, J =2.6 Hz, 1H); 13C-NMR (CDCl3) δ 169.1, 169.0, 139.6, 131.1, 129.2, 126.6, 78.2, 77.7, 73.6, 59.7, 53.4, 53.3, 45.1, 24.1, 15.4; HRMS (EI) m/z calc. for C17H19NO6S (M+) 365.0933, found 365.0928.
Dimethyl 2-(2-nitro-1-(thiophen-2-yl)ethyl)-2-(prop-2-yn-1-yl)malonate (1j): Colorless solid; Yield: 90% (2.5 h); m.p. 101–102 °C; 1H-NMR (CDCl3) δ 7.25 (d, J = 5.0 Hz, 1H), 7.00 (d, J = 3.6 Hz, 1H), 6.95 (dd, J = 5.0, 3.6 Hz, 1H), 5.26 (dd, J = 13.4, 2.6 Hz, 1H), 4.97 (dd, J = 13.4, 11.1 Hz, 1H), .4.86 (d, J = 11.1 Hz, 1H), 3.83 (s, 3H), 3.79 (s, 3H), 2.89 (dd, J = 17.5, 2.7 Hz, 1H), 2.61 (dd, J = 17.4, 2.7 Hz, 1H); 2.23 (t, J = 2.7 Hz, 1H); 13C-NMR (CDCl3) δ 168.8, 168.7, 136.8, 128.6, 127.2, 126.3, 79.1, 78.0, 73.6, 60.1, 53.6, 53.5, 41.8, 24.1; HRMS (EI) m/z calc. for C14H15NO6S (M+) 325.0620, found 325.0615.
Dimethyl 2-(1-benzo[d][1,3]dioxol-5yl)-2-nitroethyl)-2-(prop-2-yn-1-yl)malonate (1k): Colorless solid; Yield: 95% (4 h); m.p. 169–170 °C. 1H-NMR (CDCl3) δ 6.73 (d, J = 8.4 Hz, 1H), 6.67–6.65 (m, 2H), 5.95 (s, 2H), 5.24 (dd, J = 13.6, 3.1 Hz, 1H), 4.94 (dd, J = 13.6, 11.4 Hz, 1H), 4.42 (dd, J = 11.1, 3.1Hz, 1H), 3.81 (s, 3H), 3.78 (s, 3H), 2.81 (dd, J = 17.4, 2.6 Hz, 1H), 2.46 (dd, J = 17.4, 2.6 Hz, 1H); 2.17 (t, J = 2.6 Hz, 1H); 13C-NMR (CDCl3) δ 169.1, 169.0, 148.2, 148.1, 128.1, 122.6, 109.0, 108.8, 101.5, 78.2, 77.9, 73.6, 59.9, 53.5, 53.4, 45.4, 25.2; HRMS (EI) m/z calc. for C17H17NO8 (M+) 363.0954, found 363.0949.
Dimethyl 2-(1-(naphthalen-1-yl)-2-nitroethyl)-2-(prop-2-yn-1-yl)malonate (1l): Colorless solid; Yield: 77% (2 h); m.p. 145–146 °C; 1H-NMR (CDCl3) δ 8.45 (d, J = 8.6 Hz, 1H), 7.80 (t, J = 9.4 Hz, 2H), 7.54 (t, J = 7.6 Hz, 1H), 7.48 (t, J = 7.7 Hz, 1H), 7.40 (t, J = 7.6 Hz, 1H), 7.24 (d, J = 7.2 Hz, 1H), 5.56–5.48 (m, 2H), 5.22 (dd, J = 13.5, 10.9 Hz, 1H), 3.80 (s, 3H), 3.63 (s, 3H), 2.78 (dd, J = 17.5, 2.6 Hz, 1H), 2.36 (dd, J = 17.5, 2.6 Hz, 1H); 2.21 (t, J = 2.6 Hz, 1H); 13C-NMR (CDCl3) δ 169.4, 169.2, 134.1, 132.8, 131.9, 129.5, 128.8, 126.7, 126.3, 125.0, 124.8, 123.8, 79.1, 78.7, 73.3, 60.9, 53.4, 53.3, 39.2, 23.6; HRMS (EI) m/z calc. for C20H19NO6 (M+) 369.1212, found 369.1207.
Dimethyl 2-(4-methyl-1-nitropentan-2yl-)-2-(prop-2-yn-1-yl)malonate (1m): Pale yellow oil; Yield: 82% (3 h); 1H-NMR (CDCl3) δ 4.98 (dd, J = 14.5, 3.9 Hz, 1H), .4.30 (dd, J = 14.5, 5.7 Hz, 1H), 3.75 (s, 3H), 3.74 (s, 3H), 3.36–3.31 (m, 1H), 2.93 (dd, J = 17.6, 2.7 Hz, 1H), 2.84 (dd, J = 17.6, 2.6 Hz, 1H); 2.11 (t, J = 2.7 Hz, 1H), 1.58–1.51 (m, 1H), 1.31–1.18 (m, 2H), 0.93 (d, J = 6.4 Hz, 3H), 0.90 (d, J = 6.4 Hz, 3H); 13C-NMR (CDCl3) δ 169.7, 169.4, 78.5, 78.3, 72.9, 60.0, 53.2, 53.1, 39.7, 37.9, 25.7, 23.9, 23.3, 21.4;. HRMS (EI) m/z calc. for C14H21NO6 (M+) 300.1448, found 300.1444.
Dimethyl 2-(2-nitro-1-phenylethyl)-2-(3-phenylprop-2-yn-1-yl)malonate (1n): Pale yellow oil; Yield: 98% (3 h); 1H-NMR (CDCl3) δ 7.46–7.45 (m, 2H), 7.35–7.31 (m, 6H), 7.24–7.23 (m, 2H), 5.35 (dd, J = 13.7, 3.0 Hz, 1H), 5.06 (dd, J = 13.7, 11.3 Hz, 1H), 4.61 (dd, , J = 11.2, 3.0 Hz, 1H) 3.84 (s, 3H), 3.80 (s, 3H), 3.02 (d, J = 17.5 Hz, 1H), 2.63 (d, J = 17.5 Hz, 1H); 13C-NMR (CDCl3) δ 169.2, 169.1, 134.8, 131.7, 129.0, 128.8, 128.7, 128.5, 128.4, 122.8, 85.5, 83.5, 77.0, 60.1, 53.3, 53.2, 45.7, 24.9;. HRMS (EI) m/z calc. for C22H21NO6 (M+) 395.1369, found 395.1366.
Tetramethyl 2,2'-(1,4-phenylenebis(2-nitroethane-1,1-diyl))bis(2-(prop-2-yn-1-yl)malonate) (1o): Colorless solid; Yield: 60% (2 h); m.p. 180–182 °C; 1H-NMR (CDCl3) δ 7.18 (s, 4H), 5.29 (dd, J = 13.9, 3.0 Hz, 2H), 5.00 (ddd, J = 13.9, 11.1, 3.1 Hz, 2H), 4.48 (d, J = 9.9 Hz, 2H), 3.76 (s, 6H), 3.75 (s, 6H), 2.78 (dd, J = 17.4, 2.4 Hz, 2H), 2.34 (dt, J = 17.4, 3.5 Hz, 2H); 2.20 (s, 2H); 13C-NMR (CDCl3) δ 169.2, 169.1, 169.0, 135.7, 135.6, 129.5, 78.2, 78.1, 73.8, 73.7, 59.9, 53.5, 53.4, 45.4, 24.2, 24.1; HRMS (ESI) m/z calc. for C26H28N2O12 (M) + 560.1642, found 560.1642.

3.3. General Procedure for the Synthesis of 3a–3f

LHMDS (1.5 mmol) was added to a solution of dimethyl propargylmalonate (1.2 mmol) in anhydrous THF (5 mL) at 0 °C under a nitrogen atmosphere, followed by stirring for 1 h. A solution of nitroalkene (1 mmol) in anhydrous THF (5 mL) was then added drop wise over a period of five minutes at 0 °C. The reaction mixture was then stirred at the same temperature until the reaction was complete, which was monitored by TLC. After the completion of the reaction, a saturated aqueous NH4Cl was added and the resulting mixture extracted with ethyl acetate (3 × 10 mL). The combined organic phases were dried over MgSO4 and the solvent removed in vacuo. The residue was purified by flash column chromatography over silica (ethyl acetate and hexane).
Dimethyl 2-allyl-2-(1-(4-chlorophenyl)-2-nitroethyl)malonate (3b): Colorless solid; Yield: 98% (2.5 h); m.p. 98–99 °C; 1H-NMR (CDCl3) δ 7.29 (d, J = 8.4 Hz, 2H), 7.07 (d, J = 8.4 Hz, 2H), 5.75–5.65 (m, 1H), 5.15–4.91 (m, 4H), 4.18 (dd, J = 11.1, 3.1 Hz, 1H), 3.80 (s, 3H), 3.74 (s, 3H), 2.58 (dd, J =14.5 Hz, 6.4 Hz, 1H), 2.27 (dd, J =14.5 Hz, 8.1 Hz, 1H); 13C-NMR (CDCl3) δ 170.0, 169.9, 134.7, 133.6, 131.7, 130.4, 129.2, 120.2, 78.2, 60.7, 53.0, 52.9, 46.3, 38.5; HRMS (EI) m/z calc. for C16H18NO635Cl (M+) 355.0823, found 355.0817.
Dimethyl 2-allyl-2-(2-nitro-1-(p-tolyl)ethyl)malonate (3c): Colorless solid; Yield: 97% (4 h); m.p. 88–89 °C; 1H-NMR (CDCl3) δ 7.10 (d, J = 7.9 Hz, 2H), 6.98 (d, J = 7.9 Hz, 2H), 5.78–5.68 (m, 1H), 5.14–4.93(m, 4H), 4.16 (dd, J = 10.8, 3.4 Hz, 1H), 3.81 (s, 3H), 3.74 (s, 3H), 2.56 (dd, J = 14.4, 6.5 Hz, 1H), 2.30 (s, 3H) 2.29–2.25 (m, 1H); 13C-NMR (CDCl3) δ170.4, 170.2, 138.6, 132.1, 131.8, 129.7, 128.8, 120.0, 78.6, 61.1, 53.0 52.9, 46.7, 38.7, 21.2; HRMS (EI) m/z calc. for C17H21NO6 (M+) 335.1369, found 335.1363.
Dimethyl 2-allyl-2-(1-(4-methoxyphenyl)-2-nitroethyl)malonate (3d): Yellow oil; Yield: 80% (7 h); 1H-NMR (CDCl3) δ 7.02 (d, J = 8.7 Hz, 2H), 6.83 (d, J = 8.7 Hz, 2H), 5.76–5.67 (m, 1H), 5.15–4.92 (m, 4H), 4.14 (dd, J = 11.0, 3.4 Hz, 1H), 3.81 (s, 3H), 3.77 (s, 3H), 3.74 (s, 3H), 2.57 (dd, J =14.4, 8.0 Hz, 1H), 2.29 (dd, J =14.4, 8.0 Hz, 1H); 13C-NMR (CDCl3) δ 170.4, 170.2, 159.9, 132.1, 130.1, 126.7, 120.0, 114.4, 78.6, 61.2, 55.4, 53.0, 52.9, 46.4, 38.7; HRMS (EI) m/z calc. for C17H21NO7 (M+) 351.1318, found 349.1313.
Dimethyl 2-allyl-2-(2-nitro-1-(thiophen-2-yl)ethyl)malonate (3e): Yellow oil; Yield: 95% (4 h); 1H-NMR (CDCl3) δ 7.24 (d, J = 5.0 Hz, 1H), 6.93–6.90 (m, 1H), 6.89 (d, J = 5.0 Hz, 1H), 5.79–5.72 (m, 1H), 5.17–4.92 (m, 4H), 4.52 (dd, J = 10.5, 3.1 Hz, 1H), 3.81 (s, 3H), 3.74 (s, 3H), 2.69 (dd, J = 14.5, 6.5 Hz, 1H), 2.43 (dd, J = 14.5, 8.1 Hz, 1H); 13C-NMR (CDCl3) δ 169.8, 169.7, 137.1, 131.8, 128.4, 127.0, 126.1, 120.2, 79.6, 61.2, 53.0, 42.7, 38.6; HRMS (EI) m/z calc. for C14H17NO6S (M+) 327.0777, found 327.0771.
Dimethyl 2-allyl-2-(1-(naphthalen-1-yl)-2-nitroethyl)malonate (3f): Yellow oil; Yield 98% (3.5 h); 1H-NMR (CDCl3) δ 8.31 (d, J = 8.6 Hz, 1H), 7.82 (dd, J = 13.7, 8.2 Hz, 1H), 7.59 (td, J = 7.4, 0.8 Hz, 1H), 7.50 (t, J = 7.5 Hz, 1H), 7.42 (t, J = 7.6 Hz, 1H), 7.22 (d, J = 7.2 Hz, 1H), 5.75–5.65 (m, 1H), 5.35 (t, J = 7.0 Hz, 1H), 5.16 (d, J = 7.1 Hz, 2H), 5.00 (d, J = 10.1 Hz, 1H), 4.90 (d, J = 17.0 Hz, 1H), 3.85 (s, 3H), 3.73 (s, 3H), 2.38 (dd, J = 14.2, 6.6 Hz, 1H), 2.23 (dd, J = 14.2, 7.9 Hz, 1H); 13C-NMR (CDCl3) δ 170.6, 170.4, 134.2, 133.1, 132.7, 132.2, 129.4, 129.1, 127.0, 126.3, 125.2, 124.6, 123.5, 119.6, 79.4, 62.8, 52.9, 40.6, 39.1; HRMS (EI) m/z calc. for C20H21NO6 (M+) 371.1369, found 371.1363.

3.4. General Procedure for the Preparation of 2a–2o and 4a–4f

A mixture of allyl or propargyl dimethyl malonate (1 mmol) and DBU (1.5 equiv.) in anhydrous dichloromethane (3 mL) was stirred at −78 °C for 20 min under nitrogen. To this mixture, the Yamaguchi reagent (1.5 equiv.) and ZrCl4 (10 mol %) were added. The reaction mixture was then stirred at the same temperature for 10 min. then stopped the cooler and continued until the reaction was complete, as indicated by TLC. After the completion of the reaction, the reaction mixture was washed with water, and extracted with CH2Cl2, washed the organic layer with brine solution. The organic layers were collected, combined, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The residue was then purified by column chromatography using EtOAc-hexane to give the desired product.
2a, 2j, 2k, 4a–4f are known compounds [43].
Dimethyl-6-(2-bromophenyl)-4H-cyclopenta[c]isoxazole-5,5(6H)-dicarboxylate (2b): Brown solid; m.p. 127–129 °C; 1H-NMR (CDCl3) δ 8.10 (s, 1H), 7.58 (d, J = 7.9 Hz, 1H), 7.16 (t, J = 7.4 Hz,1H), 7.08 (td, J = 7.7, 1.3 Hz, 1H),6.73 (d, J = 7.6 Hz, 1H), 5.97 (s, 1H), 3.88 (d, J = 17.2 Hz, 1H), 3.80 (s, 3H), 3.29 (s, 3H), 3.25 (d, J = 17.2 Hz, 1H); 13C-NMR (CDCl3) δ 172.8, 170.9, 168.2, 150.8, 137.1, 133.2, 129.8, 129.5, 127.8, 125.5, 121.3, 72.6, 53.7, 52.5, 46.9, 29.9; HRMS (EI) m/z calc. for C16H1479Br NO5 (M+) 379.0055, found 379.0050.

Dimethyl-6-(o-tolyl)-4H-cyclopenta[c]isoxazole-5,5(6H)-dicarboxylate (2c): White solid; m.p. 117–118 °C; 1H-NMR (CDCl3) δ 8.08 (s, 1H), 7.16–7.08 (m, 2H), 7.03 (t, J = 7.4 Hz, 1H), 6.97 (d, J = 7.4 Hz, 1H), 5.63 (s, 1H), 3.63 (d, J = 17.4, 1.5 Hz, 1H), 3.79 (s, 3H), 3.25 (d, J = 17.4, 1.4Hz, 1H), 3.19 (s, 3H), 2.49 (s, 3H); 13C-NMR (CDCl3) δ 173.5, 171.5, 168.5, 150.5, 137.1, 135.8, 130.6, 128.3, 127.9, 126.3, 121.4, 72.9, 53.6, 52.4, 44.0, 29.8, 20.2. HRMS (EI) m/z calc. for C17H17NO5Na (M+ + Na) 338.1005, found 338.1015.

Dimethyl-6-(2-methoxyphenyl)-4H-cyclopenta[c]isoxazole-5,5(6H)-dicarboxylate (2d): Pale yellow oil; 1H-NMR (CDCl3) δ 8.02 (s, 1H), 7.21 (td, J = 7.8, 1.5 Hz, 1H), 6.97 (d, J = 6.8 Hz, 1H), 6.86–6.81 (m, 2H), 5.63 (s, 1H), 3.88 (dd, J = 16.7, 1.2 Hz, 1H), 3.78 (s, 3H), 3.72 (s, 3H), 3.22 (s, 3H), 3.16 (d, J = 16.7 Hz, 1H); 13C-NMR (CDCl3) δ 172.9, 171.6, 168.7, 157.4, 149.8, 130.5, 129.4, 125.8, 121.6, 120.7, 111.1, 72.3, 55.6, 53.5, 52.4, 43.5, 30.4; HRMS (EI) m/z calc. for C17H17NO6 (M+) 331.1056, found 331.1064.
Dimethyl-6-(4-fluorophenyl)-4H-cyclopenta[c]isoxazole-5,5(6H)-dicarboxylate (2e): Pale yellow oil; 1H-NMR (CDCl3) δ 8.10 (s, 1H), 7.21 (dd, J = 8.0, 3.9 Hz, 1H), 6.97 (t, J =8.4 Hz, 3H), 5.31 (s, 1H), 3.80 (s, 3H), 3.67 (d, J = 16.4 Hz, 1H), 3.24 (s, 3H), 3.11 (d, J = 16.8 Hz, 1H); 13C-NMR (CDCl3) δ 171.8, 171.0, 168.9, 162.6 (d, J = 245 Hz), 150.6, 131.8 (d, J = 3 Hz), 130.5 (d, J = 8 Hz), 121.6, 115.5 (d, J = 21 Hz), 72.9, 53.5, 48.0, 29.7; HRMS (EI) m/z calc. for C16H14FNO5 (M+) 319. 0856, found 319.0851.
Dimethyl-6-(4-chlorophenyl)-4H-cyclopenta[c]isoxazole-5,5(6H)-dicarboxylate (2f): Pale yellow solid; m.p. 124–125°C; 1H-NMR (CDCl3) δ 8.11 (s, 1H), 7.26 (d, J = 8.1 Hz, 2H), 7.17 (d, J = 8.1 Hz, 2H), 5.30 (s, 1H), 3.81 (s, 3H), 3.68 (d, J = 16.6 Hz, 1H), 3.25 (s, 3H), 3.13 (d, J = 16.6 Hz, 1H); 13C-NMR (CDCl3) δ 171.5, 170.9, 168.8, 150.6, 134.6, 134.2, 130.5, 128.7, 121.4, 72.9, 53.6, 52.7, 48.1, 29.7; HRMS (EI) m/z calc. for C16H1435ClNO5 (M+) 335. 056, found 335.0555.
Dimethyl-6-(4-methoxyphenyl)-4H-cyclopenta[c]isoxazole-5,5(6H)-dicarboxylate (2g): White solid; m.p. 111–110 °C; 1H-NMR (CDCl3) δ 8.06 (s, 1H), 7.09 (d, J = 8.6 Hz, 2H), 6.78 (d, J = 8.64 Hz, 2H), 5.24 (s, 1H), 3.77 (s, 3H), 3.74 (s, 3H), 3.66 (d, J = 16.5 Hz, 1H), 3.23 (s, 3H), 3.10 (d, J = 16.5 Hz, 1H); 13C-NMR (CDCl3) δ 172.2, 171.1, 168.9, 159.4, 150.3, 130.1, 128.0, 121.2, 113.9, 72.8, 55.3, 53.4, 52.5, 48.0, 29.5; HRMS (EI) m/z calc. for C17H17NO6 (M+) 331.1056, found 331.1050.
Dimethyl-6-(p-tolyl)-4H-cyclopenta[c]isoxazole-5,5(6H)-dicarboxylate (2h): White solid; m.p. 102–103 °C; 1H-NMR (CDCl3) δ 8.00 (s, 1H), 7.01 (d, J = 8.2 Hz, 2H), 6.98 (d, J = 8.2 Hz, 2H), 5.19 (s, 1H), 3.72 (s, 3H), 3.63 (d, J = 16.6 Hz, 1H), 3.17 (s, 3H), 3.06 (d, J = 16.6 Hz, 1H), 2.22 (s, 3H); 13C-NMR (CDCl3) δ 171.9, 170.9, 168.5, 150.1, 137.6, 132.8, 128.9, 128.6, 121.1, 72.7, 53.2, 52.3, 48.1, 29.4, 21.0; HRMS (EI) m/z calc. for C17H17NO5 (M+) 315.1107, found 315.1101.
Dimethyl-6-(4-(methylthio)phenyl)-4H-cyclopenta[c]isoxazole-5,5(6H)-dicarboxylate (2i): Orange solid; m.p. 114–116 °C; 1H-NMR (CDCl3) δ 8.09 (s, 1H), 7.16 (d, J = 8.4 Hz, 2H), 7.11 (d, J = 8.4 Hz, 2H), 5.27 (s, 1H), 3.79 (s, 3H), 3.69 (dd, J = 16.0, 1.0 Hz, 1H), 3.25 (s, 3H), 3.12 (dd, J = 16.0, 1.0 Hz, 1H), 2.44 (s, 3H); 13C-NMR (CDCl3) δ 171.9, 171.1, 168.8, 150.5, 138.7, 132.8, 129.5, 126.6, 121.3, 72.9, 53.5, 52.6, 48.3, 29.7, 15.9; HRMS (EI) m/z calc. for C17H17NO5S (M+) 347. 0827, found 347.0822.
Dimethyl-6-(thiophen-2-yl)-4H-cyclopenta[c]isoxazole-5,5(6H)-dicarboxylate (2j): White solid; m.p. 129–130 °C; 1H-NMR (CDCl3) δ 8.08 (s, 1H), 7.23–7.21(m, 1H), 6.94–6.91 (m, 2H), 5.53(s, 1H), 3.81 (s, 3H), 3.68 (dd, J = 16.5, 1.2Hz, 1H), 3.41 (s, 3H), 3.14 (dd, J = 16.5, 1.2 Hz, 1H); 13C-NMR (CDCl3) δ 171.5, 170.7, 168.5, 150.6, 137.4, 127.5, 126.8, 125.9, 120.6, 72.9, 53.5, 52.8, 44.1, 29.3; HRMS (EI) m/z calc. for C14H13NO5S (M+) 307. 0514, found 307.0513.
Dimethyl-6-(benzo[d][1,3]dioxol-5-yl)-4H-cyclopenta[c]isoxazole-5,5(6H)-dicarboxylate (2k): White solid; m.p. 93–94 °C; 1H-NMR (CDCl3) δ 8.08 (s, 1H), 6.72–6.66 (m, 3H), 5.91–5.90 (m, 2H), 5.23 (s, 1H), 3.79 (s, 3H), 3.66 (d, J = 16.5 Hz, 1H), 3.33 (s, 3H), 3.11 (d, J = 16.5 Hz, 1H); 13C-NMR (CDCl3) δ 171.9, 171.0, 168.7, 150.4, 147.7, 129.5, 122.5, 121.2, 109.4, 108.1, 101.2, 72.7, 53.4, 52.6, 48.3, 29.5; HRMS (EI) m/z calc. for C17H15NO7 (M+) 345. 0849, found 345.0847.
Dimethyl-6-(naphthalen-1-yl)-4H-cyclopenta[c]isoxazole-5,5(6H)-dicarboxylate (2l): White solid; m.p. 131–133 °C; 1H-NMR (CDCl3) δ 8.39 (d, J =5.4 Hz, 1H), 8.15 (s, 1H), 7.83 (d, J =8.1 Hz, 1H), 7.75 (d, J=8.2 Hz, 1H), 7.58 (t, J =6.8 Hz, 1H), 7.49 (t, J = 7.6 Hz, 1H), 7.33 (t, J = 7.6 Hz, 1H), 6.91 (s, 1H), 6.31 (s, 1H), 3.91 (d, J = 16.9 Hz, 1H), 3.83 (s, 3H), 3.31 (d, J = 16.9 Hz, 1H), 2.71 (s, 3H); 13C-NMR (CDCl3) δ 173.3, 171.4, 168.2, 150.6, 133.9, 133.5, 131.9, 128.8, 128.7, 126.6, 126.3, 125.9, 125.3, 124.2, 121.6, 73.0, 53.7, 52.0, 43.2, 29.8; HRMS (EI) m/z calc. for C20H17NO5 (M+) 351.1106, found 351.1101.
Dimethyl-6-isobutyl-4H-cyclopenta[c]isoxazole-5,5(6H)-dicarboxylate (2m): White solid; m.p. 101–102 °C; 1H-NMR (CDCl3) δ 7.93 (s, 1H), 3.95 (dd, J = 11.7, 4.2 Hz, 1H), 3.76 (s, 3H), 3.75 (s, 3H), 3.46 (dd, J = 16.4, 1.5 Hz, 1H), 3.08 (dd, J =16.4, 1.5 Hz, 1H), 2.03–1.96 (m, 1H), 1.41 (td, J =12.9, 3.7 Hz, 1H), 1.33–1.26 (m, 1H), 1.04 (d, J = 6.5 Hz, 3H), 0.93 (d, J = 6.5 Hz, 3H); 13C-NMR (CDCl3) δ 172.4, 171.2, 169.7, 149.5, 120.1, 70.4, 53.3, 52.9, 41.7, 38.0, 29.4, 25.8, 24.0, 21.2; HRMS (EI) m/z calc. for C20H17NO5 (M+) 281.1263, found 281.1258.
Dimethyl-6-(2-bromophenyl)-4H-cyclopenta[c]isoxazole-5,5(6H)-dicarboxylate (2n): White solid; m.p. 209–211 °C; 1H-NMR (CDCl3) δ 7.76–7.74 (m, 2H), 7.51–7.41 (m, 3H), 7.31–7.22 (m, 5H), 5.35 (s, 1H), 3.94 (d, J = 16.7 Hz, 1H), 3.81 (s, 3H), 3.24 (d, J = 16.7 Hz, 1H), 3.22 (s, 3H); 13C-NMR (CDCl3) δ 173.5, 171.2, 168.8, 161.3, 136.3, 130.1, 129.3, 129.2, 128.2, 127.8, 126.3, 116.9, 73.3, 53.6, 52.6, 49.0, 31.1; HRMS (EI) m/z calc. for C22H19NO5 (M+) 377.1263, found 377.1265.

4. Conclusions

In summary we report on a practical and efficient regioselective synthesis of bicyclic isoxazole/isoxazoline derivatives via intramolecular nitrile oxide cycloaddition reactions using the Yamaguchi reagent and DBU in the presence of ZrCl4. The process is straightforward, easy to perform and does not involve the use of costly reagents or catalysts. A wide variety of carbocyclic fused isoxazole and isoxazoline derivatives were synthesized in good to moderate yields.

Acknowledgments

Financial support by the Ministry of Science and Technology of the Republic of China (MOST 103-2113-M-003-008-MY3) National Taiwan Normal University (103-07-C) and the Instrumentation Centre at National Taiwan Normal University is gratefully acknowledged. The authors are grateful to Hsiu-Ni Huan, Mei-Ling Chen and Chiu-Hui He for providing mass, HRMS and NMR spectral data.

Author Contributions

Ching-Fa Yao, Wen-Chang Chen, Veerababurao Kavala, conceived and designed the experiments; Wen-Chang Chen, Yu-Hsuan Shih, Yu-Hsuan Wang, Chun-Wei Kuo, Tang-Hao Yang and Chia-Yu Huang, performed the experiments; Chia-Yu Huang, Hao-Hsiang Chiu analyzed the data; Veerababurao Kavala and Ching-Fa Yao wrote the paper.

Conflicts of Interest

The authors declare no conflict of interest.

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  • Sample Availability: Samples of the compounds 2a–2o and 4a–4f are available from the authors.

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Chen, W.-C.; Kavala, V.; Shih, Y.-H.; Wang, Y.-H.; Kuo, C.-W.; Yang, T.-H.; Huang, C.-Y.; Chiu, H.-H.; Yao, C.-F. Synthesis of Bicyclic Isoxazoles and Isoxazolines via Intramolecular Nitrile Oxide Cycloaddition. Molecules 2015, 20, 10910-10927. https://doi.org/10.3390/molecules200610910

AMA Style

Chen W-C, Kavala V, Shih Y-H, Wang Y-H, Kuo C-W, Yang T-H, Huang C-Y, Chiu H-H, Yao C-F. Synthesis of Bicyclic Isoxazoles and Isoxazolines via Intramolecular Nitrile Oxide Cycloaddition. Molecules. 2015; 20(6):10910-10927. https://doi.org/10.3390/molecules200610910

Chicago/Turabian Style

Chen, Wen-Chang, Veerababurao Kavala, Yu-Hsuan Shih, Yu-Hsuan Wang, Chun-Wei Kuo, Tang-Hao Yang, Chia-Yu Huang, Hao-Hsiang Chiu, and Ching-Fa Yao. 2015. "Synthesis of Bicyclic Isoxazoles and Isoxazolines via Intramolecular Nitrile Oxide Cycloaddition" Molecules 20, no. 6: 10910-10927. https://doi.org/10.3390/molecules200610910

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