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Molecules 2015, 20(3), 3549-3564;

Anti-Glycation Activities of Phenolic Constituents from Silybum marianum (Milk Thistle) Flower in Vitro and on Human Explants

Biospectrum Life Science Institute, Eines Platz 11th FL, 442-13 Sangdaewon Dong, Seoungnam City, Gyunggi Do 462-807, Korea
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 25 November 2014 / Revised: 13 February 2015 / Accepted: 15 February 2015 / Published: 19 February 2015
(This article belongs to the Section Natural Products Chemistry)
Full-Text   |   PDF [1309 KB, uploaded 19 February 2015]   |  


Glycation is an ageing reaction of naturally occurring sugars with dermal proteins, with clinical signs appearing in vivo around age 30, and increasing steadily/regularly with age. The suppleness of the dermis is affected by the formation of bridges between proteins and sugars (Maillard’s reaction). The accumulation of advanced glycation end products (AGEs) in skin plays a very important role in skin ageing. Therefore, natural compounds or extracts that possess antiglycation activities may have great anti-ageing potential. In the present study, Silybum marianum flower extract (SMFE) was demonstrated to possess antiglycation activity. We found that SMFE inhibits glycation reaction between BSA and glucose. In addition, antiglycation activity of SMFE was confirmed in a human skin explants model. SMFE reduced Nε-(carboxymethyl) lysine (CML) expression, whereas SMFE stimulated fibrillin-1 expression compared to treatment with methyglyoxal. An active ingredient contributing to the observed activities was identified as silibinin. The antiglycation activity of silibinin was dose-dependent. The beneficial effects of silibinin may be applied to prevention or management of AGE-mediated pathologies, targeting in a pleiotropic and complementary way the biochemical and cellular bases of skin aging. View Full-Text
Keywords: AGE; fibrillin-1; glycation; human skin explants; silibinin AGE; fibrillin-1; glycation; human skin explants; silibinin

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Shin, S.; Lee, J.-A.; Kim, M.; Kum, H.; Jung, E.; Park, D. Anti-Glycation Activities of Phenolic Constituents from Silybum marianum (Milk Thistle) Flower in Vitro and on Human Explants. Molecules 2015, 20, 3549-3564.

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