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Open AccessArticle

Characterisation of Mediterranean Grape Pomace Seed and Skin Extracts: Polyphenolic Content and Antioxidant Activity

1
Univ Bordeaux, ISVV, EA 4577 Œnologie, 210 Chemin de Leysotte, Villenave d'Ornon F-33140, France
2
INRA, ISVV, USC 1366 Œnologie, 210 Chemin de Leysotte, Villenave d'Ornon F-33140, France
*
Author to whom correspondence should be addressed.
Academic Editor: Maurizio Battino
Molecules 2015, 20(2), 2190-2207; https://doi.org/10.3390/molecules20022190
Received: 25 November 2014 / Revised: 8 January 2015 / Accepted: 19 January 2015 / Published: 29 January 2015
(This article belongs to the Special Issue Natural Antioxidants and Ageing)
Grape pomace seeds and skins from different Mediterranean varieties (Grenache [GRE], Syrah [SYR], Carignan [CAR], Mourvèdre [MOU] and Alicante [ALI]) were extracted using water and water/ethanol 70% in order to develop edible extracts (an aqueous extract [EAQ] and a 70% hydro-alcoholic extract [EA70]) for potential use in nutraceutical or cosmetic formulations. In this study, global content (total polyphenols, total anthocyanins and total tannins), flavan-3-ols and anthocyanins were assessed using HPLC-UV-Fluo-MSn. In addition, extract potential was evaluated by four different assays: Oxygen Radical Absorbance Capacity (ORAC), Ferric Reducing Antioxidant Potential assay (FRAP), Trolox equivalent antioxidant capacity (TEAC) or ABTS assay and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. As expected, seed pomace extracts contained higher amounts of polyphenols then skin pomace extracts. Indeed, seeds from Syrah contained a particularly important amount of total polyphenols and tannins in both type of extract (up to 215.84 ± 1.47 mg of gallic acid equivalent [GAE]/g dry weight (DW) and 455.42 ± 1.84 mg/g DW, respectively). These extracts also expressed the highest antioxidant potential with every test. For skins, the maximum total phenolic was found in Alicante EAQ (196.71 ± 0.37 mg GAE/g DW) and in Syrah EA70 (224.92 ± 0.18 mg GAE/g DW). Results obtained in this article constitute a useful tool for the pre-selection of grape pomace seed and skin extracts for nutraceutical purposes. View Full-Text
Keywords: grape pomaces; Mediterranean varieties; phenolic compounds; flavan-3-ols; anthocyanins; antioxidant activity grape pomaces; Mediterranean varieties; phenolic compounds; flavan-3-ols; anthocyanins; antioxidant activity
MDPI and ACS Style

Ky, I.; Teissedre, P.-L. Characterisation of Mediterranean Grape Pomace Seed and Skin Extracts: Polyphenolic Content and Antioxidant Activity. Molecules 2015, 20, 2190-2207.

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