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Molecules 2015, 20(11), 20777-20804;

DNA Catalysis: The Chemical Repertoire of DNAzymes

Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, Switzerland
Present address: Institut Pasteur, Chemistry and Structural Biology Department, Laboratory for Bioorganic Chemistry of Nucleic Acids, 28 rue du Dr Roux, 75724 Paris cedex 15, France.
Academic Editor: Hui Wei
Received: 25 October 2015 / Revised: 10 November 2015 / Accepted: 11 November 2015 / Published: 20 November 2015
(This article belongs to the Special Issue Nanozymes and Beyond)
Full-Text   |   PDF [5193 KB, uploaded 20 November 2015]   |  


Deoxyribozymes or DNAzymes are single-stranded catalytic DNA molecules that are obtained by combinatorial in vitro selection methods. Initially conceived to function as gene silencing agents, the scope of DNAzymes has rapidly expanded into diverse fields, including biosensing, diagnostics, logic gate operations, and the development of novel synthetic and biological tools. In this review, an overview of all the different chemical reactions catalyzed by DNAzymes is given with an emphasis on RNA cleavage and the use of non-nucleosidic substrates. The use of modified nucleoside triphosphates (dN*TPs) to expand the chemical space to be explored in selection experiments and ultimately to generate DNAzymes with an expanded chemical repertoire is also highlighted. View Full-Text
Keywords: SELEX; DNAzymes; chemically modified nucleic acids; functional nucleic acids; biosensors; therapeutic nucleic acids SELEX; DNAzymes; chemically modified nucleic acids; functional nucleic acids; biosensors; therapeutic nucleic acids

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Hollenstein, M. DNA Catalysis: The Chemical Repertoire of DNAzymes. Molecules 2015, 20, 20777-20804.

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