Synthesis, Molecular Structure, Metabolic Stability and QSAR Studies of a Novel Series of Anticancer N-Acylbenzenesulfonamides

Żołnowska, Beata; Sławiński, Jarosław; Belka, Mariusz; Bączek, Tomasz; Kawiak, Anna; Chojnacki, Jarosław; Pogorzelska, Aneta; Szafrański, Krzysztof

doi:10.3390/molecules201019101

Open AccessArticle

Synthesis, Molecular Structure, Metabolic Stability and QSAR Studies of a Novel Series of Anticancer N-Acylbenzenesulfonamides

by

Beata Żołnowska

¹,

Jarosław Sławiński

^1,*

,

Mariusz Belka

²,

Tomasz Bączek

²,

Anna Kawiak

^3,4,

Jarosław Chojnacki

⁵

,

Aneta Pogorzelska

¹ and

Krzysztof Szafrański

¹

Department of Organic Chemistry, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416 Gdańsk, Poland

²

Department of Pharmaceutical Chemistry, Medical University of Gdańsk, Al. Gen. J. Hallera 107, 80-416 Gdańsk, Poland

³

Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, ul. Kładki 24, 80-822 Gdańsk, Poland

⁴

Department of Human Physiology, Medical University of Gdańsk, ul. Tuwima 15, 80-210 Gdańsk, Poland

⁵

Department of Inorganic Chemistry, Gdańsk University of Technology, Narutowicza 11/12, 80-233 Gdańsk, Poland

^*

Author to whom correspondence should be addressed.

Molecules 2015, 20(10), 19101-19129; https://doi.org/10.3390/molecules201019101

Submission received: 9 September 2015 / Revised: 1 October 2015 / Accepted: 12 October 2015 / Published: 21 October 2015

(This article belongs to the Section Medicinal Chemistry)

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Abstract

:

A series of novel N-acyl-4-chloro-5-methyl-2-(R¹-methylthio)benzenesulfonamides 18–47 have been synthesized by the reaction of N-[4-chloro-5-methyl-2-(R1-methylthio)benzenesulfonyl]cyanamide potassium salts with appropriate carboxylic acids. Some of them showed anticancer activity toward the human cancer cell lines MCF-7, HCT-116 and HeLa, with the growth percentages (GPs) in the range from 7% to 46%. Quantitative structure-activity relationship (QSAR) studies on the cytotoxic activity of N-acylsulfonamides toward MCF-7, HCT-116 and HeLa were performed by using topological, ring and charge descriptors based on the stepwise multiple linear regression technique (MLR). The QSAR studies revealed three predictive and statistically significant models for the investigated compounds. The results obtained with these models indicated that the anticancer activity of N-acylsulfonamides depends on topological distances, number of ring system, maximum positive charge and number of atom-centered fragments. The metabolic stability of the selected compounds had been evaluated on pooled human liver microsomes and NADPH, both R¹ and R² substituents of the N-acylsulfonamides simultaneously affected them.

Keywords:

sulfonamide; synthesis; anticancer; QSAR; metabolic stability

Graphical Abstract

1. Introduction

Chemotherapeutics are the most important tool in the fight against cancer. For decades scientists have discovered many anticancer drugs by designing low-molecular weight compounds with the ability to bind to a preselected protein target or by screening small molecules for their ability to modulate a biological pathway in cells or organisms, or without regard for any particular protein target [1,2]. However, the question remains whether these drugs will offer lasting survival advantages to the patients in the light of the problem with of tumor unpredictability and drug resistance. A promising solution that is a focus of contemporary medicinal chemistry is the development of potent and selective anticancer drugs. Many of these new agents are being tested in combination with therapies currently used to treat specific cancers and usually include cytotoxic drugs. On the other hand, these newer agents are affording novel ways to mechanistically attack cancer, even if one cannot realize efficacy without toxicity [2].

N-Acylsulfonamides constitute a class of organic compounds with various types of pharmacological activities such as antibacterial inhibitors for tRNA synthetase [3], antagonists for angiotensin II [4], agents for the treatment of Alzheimer’s desease [5], and osteoporosis [6]. In the last decade, particular attention has been focused on N-acylsulfonamides as cyclooxygenase [7], tubulin [8] and cyclin-dependent kinases [9] inhibitors with antiproliferative activity.

Among the N-acylsulfonamides described in the literature so far, at least four have been clinically investigated as drug candidates with cytostatic activity against tumors: LY573636-sodium (tasisulam sodium) [10,11], ABT-737 [12], ABT-263 (navitoclax) [13,14] and ABT-199 [15] (Figure 1). LY573636-sodium acts an apoptosis inducer via the intrinsic pathway and exhibits antiangiogenesis activity, whereas ABT-737/263/199 are inhibitors of antiapoptotic Bcl-2 family members [10,11,12,13,14,15]. It should be noted that other acylsulfonamide analogues identified during the development of LY573636 have been reported to exhibit considerable antitumor activity, particularly against human colon, lung, prostate, ovarian, and breast xenografts growing in immunodeficient mice [16,17]. The current state of clinical trials concerning only LY573636-sodium or ABT-737/263/199, without combination with other drugs, is presented in Table 1 [18].

Our systematic studies on aryl- and heteroarylsulfonamides have resulted in promising anticancer agents [19,20,21,22,23,24], among which we have reported some N-acylsulfonamides (I, Figure 2) with antiproliferative activity against a broad spectrum of cancer cell lines [23]. Searching for innovative low-molecular chemotherapeutics we designed a series of new N-acylsulfonamides II (Figure 2) with potential anticancer activity.

Figure 1. Clinically evaluated antitumor N-acylsulfonamides: LY573636-sodium (Tasisulam sodium) and ABT-737, ABT-263, ABT-199 (inhibitors targeting Bcl-2 family members).

Table 1. Clinical Trial Information about LY573636-sodium, ABT-737, ABT-263 and ABT-199 [18].

**Table 1.** Clinical Trial Information about LY573636-sodium, ABT-737, ABT-263 and ABT-199 [18].
Drug	Conditions	Study Start Date–Study Completion Date	Phase
LY573636-sodium	Brest cancer	2009‒2011	II
	Sarcoma, Soft Tissue	2007‒2010	II
	Ovarian Cancer	2007‒2012	II
	Non-Small-Cell Lung Cancer	2006‒2008	II
	Metastatic Melanoma	2006‒ongoing	II
ABT-737	Breast cancer	2013‒withdrawn	I/II
ABT-737	Ovarian cancer	2010‒2013	II
ABT-263	Chronic Lymphoid Leukemia, Lymphoid Malignancies, NoN–Hodgkin’s Lymphoma, Follicular Lymphoma, Mantle Cell, Lymphoma, Peripheral T-cell Lymphoma	2006‒ongoing	II
	Chronic Lymphocytic, Leukemia, Lymphomas	2008‒2010	I
	Lymphoid Malignancies, Solid Tumors	2009‒2010	I
	Chronic Lymphocytic Leukemia	2007‒ongoing	II
	Chronic Lymphocytic Leukemia	2012‒2013	II
	Small Cell Lung Cancer	2007‒2010	I
	Small Cell Lung Carcinoma	2007‒2010	II
ABT-199	NoN–Hodgkin’s Lymphoma	2013‒ongoing	I
	Chronic Lymphocytic Leukemia	2013‒ongoing	I
	Relapsed/Refractory Multiple Myeloma	2012‒ongoing	I
	Acute Myelogenous/Myeloid Leukemia	2013‒2014	II
	Chronic Lymphocytic Leukemia	2014‒ongoing	II
	Chronic Lymphocytic Leukemia harboring the 17p Deletion, Cancer of the Blood and Bone Marrow	2013‒ongoing	II

Figure 2. Structures of N-acylsulfonamides I and II.

To explain how structural features influence the biological activities the quantitative structure-activity relationship (QSAR) method was applied [25]. Metabolic stability studies, one of the parameters characterizing the pharmacokinetic properties of a molecule, was performed using pooled human liver microsomes and NADPH.

2. Results and Discussion

2.1. Chemistry

The starting substrates, 3-aminobenzodithiazine 1, dipotassium salt 2, as well as potassium salts 3‒9, were obtained according to the reported procedures for preparation of N-(benzenesulfonyl)cyanamide potassium salts [22,26,27]. Novel substrates 10‒17 were synthesized analogously by nucleophilic substitution of arylmethyl chloride with dipotassium salt 2 as was shown in Scheme 1. The final N-acylbenzenesulfonamides 18–47 were achieved in the one-step reaction of N-[4-chloro-5-methyl-2-(R¹-methylthio)benzenesulfonyl]cyanamide potassium salts with appropriate carboxylic acids under reflux. For solid acid reagents (i.e., trans-cinnamic and benzoic acids) reactions were ran in water or toluene, whereas for liquid reactants (acetic, propionic, isobutyric, cyclohexylpropionic acids) syntheses were carried out without solvent.

Scheme 1. Synthesis of N-acyl-2-methylthio-4-chloro-5-methylbenzenesulfonamides 18–47. Reagents and conditions: (a) K₂CO₃ excess, THF, reflux, 24 h; (b) R¹CH₂Cl/Br, water or methanol, r.t.; (c) PhCH₂Cl, K₂CO₃ excess, THF, reflux, 24 h; (d) R²COOH, reflux (for 45–47 water or toluene).

The proposed mechanism for the formation of final N-acylsulfonamides 18–47 is outlined in Scheme 2. The initial step we believe is the protonation of the nitrile nitrogen atom by the carboxylic acid with simultaneous acceptance of the lone pair of electrons from the carboxylate anion (Step 1). Then, the nitrogen atom attached to the sulfonyl group attacks the carbonyl carbon atom causing C–O bond cleavage of the carboxylate group (Step 2). In the final step the proton from the =N–H group is transferred to the deprotonated sulfonamide nitrogen atom with simultaneous potassium cyanate elimination (Step 3).

Scheme 2. Proposed mechanism for the formation of N-acylbenzenesulfonamide derivatives 18–47.

Many methods are known for the acylation of sulfonamides. N-Acylbeznenesulfonamides can be prepared, for instance, by the reaction of a sulfonamide with a carboxylic acid in the presence of coupling reagents such as carbodiimides (DCC, EDCI), in which the by-product must be removed from the reaction mixture [8,28]. The N-acylation of sulfonamides has also based on the reaction with mixed anhydrides [9], acyl chlorides [29] or N-acylbenzotriazoles [30] in basic reaction media as well as under acidic medium with carboxylic acids or anhydrides in the presence of Lewis acid as a catalyst [31].

In the present study we utilized a new and facile method for the synthesis of N-acylated sulfonamides using N-(benzenesulfonyl)cyanamide potassium salts which can be applied as a convenient alternative to the known methods. Basic advantages of described approach include low cost, absence of coupling reagents and harmful substances, and easy isolation of the products.

The structures of the final compounds 18‒47 were confirmed by IR, ¹H-NMR and ¹³C-NMR spectroscopy. IR spectra showed the absence of the nitrile (C≡N) group and the presence of bands in the 1683‒1734 cm⁻¹ range corresponding to the stretching vibration of the carbonyl group (C=O). This functional group was also identified in the ¹³C-NMR spectra where the N-acylbeznenesulfonamide moiety displays one carbon signal at 163.43‒180.90 ppm coming from the carbonyl carbon atom. The appearance of NH signals at 12.20‒12.82 ppm in the ¹H-NMR spectra proved the presence of the amide group and also conformed the proposed structure of the final compounds. Moreover, X-ray analysis was done to confirm proposed structures on the representative compound 22.

Details on data collection, structure solution and refinement are given in Table 2. Compound 22 crystallized in the monoclinic system, the space group P2₁/c, with one molecule in the asymmetric unit. Atom numbering scheme was presented in Figure 3. The trifluoromethyl group was refined as disordered over two positions with probabilities 0.746(16)/0.254(16). The molecule is bent at the –CH₂–S– bridge in such a way that the acyl group is placed over the Ph–CF₃ ring.

Table 2. Crystal data and structure refinement details for 22.

**Table 2.** Crystal data and structure refinement details for 22.
Empirical Formula	C₁₇H₁₅ClF₃NO₃S₂
Formula weight	437.87
Temperature/K	293 (2)
Wavelength/Å	0.71073
Crystal system	Monoclinic
Space group	P2₁/c
Unit cell dimensions
a/Å	13.0071 (9)
b/Å	7.7047 (5)
c/Å	23.1637 (19)
α/°	90
β/°	120.288 (6)
γ/°	90
Volume/Å³	2004.5 (3)
Z	4
Density (calculated)/Mgm⁻³	1.451
Absorption coefficient/mm⁻¹	0.44
F(000)	896
Crystal size/mm³	0.41 × 0.18 × 0.05
θ range for data collection/°	2.83–25.2
Index ranges	−15 ≤ h ≤ 15, −9 ≤ k ≤ 9, −26 ≤ l ≤ 27
Reflections collected	12,224
Independent reflections	3605 (R_int = 0.031)
Completeness to θ = 50°	99.9%
Absorption correction	Multi-scan
Refinement method	Full-matrix least-squares on F²
Data/restraints/parameters	3605/16/278
Goodness of fit on F²	1.024
Final R indices [I > 2σ(I)]	R₁ = 0.0499, wR₂ = 0.1222
R indices (all data)	R₁ = 0.0635, wR₂ = 0.1364
Largest diff. peak and hole/eÅ⁻³	0.32, −0.30

The valence angle at S2, ca. 103° is typical for a thioether group. Notably, the S–C bond length to aliphatic carbon is longer than the bond to aromatic ring, which may indicate to some mesomeric effect. Since the sulfonamide group is acylated only one N–H hydrogen-bond donor is available. The intermolecular hydrogen bonding, of the N–H…O type, forms infinite chains (graph set symbol C(4), [32]) spreading along the b axis (Figure 4, Table 3). The chain propagates exactly along the crystal screw axis 2₁ so it is described by the 𝓅112₁ (R9) rod group symmetry [33].

Figure 3. Atom numbering scheme for 22, hydrogen atoms not labelled for clarity. Less populated fluorine atoms in disordered part of –CF₃ shown as small, disconnected balls. Selected bond lengths [Å] and angles [°]: S1-O1 1.438(2), S1-O2 1.430(2), S1-N1 1.649(3), S1 C1 1.775(2), N1-C16 1.400(4), C16-O3 1.211(4), S2-C2 1.787(3), S2-C8 1.836(3); O1-S1-O2 119.3(1), O1-S1-N1 109.0(1), O2-S1-N1 105.0(1), C2-S2-C8 103.4(1), S1-N1-C16 124.6(2), N1-C16-C17 114.4(3), S2-C8-C9 114.1(2).

Figure 4. Chain of molecules linked by hydrogen bonding and spreading along the screw 2₁ axis, parallel to the b axis (½ y ¼).

Table 3. Hydrogen bond geometry (Å, °).

**Table 3.** Hydrogen bond geometry (Å, °).
D–H···A	D–H	H···A	D···A	D–H···A
N1–H1N···O1 ⁱ	0.865 (18)	2.028 (19)	2.887 (3)	172 (3)

Symmetry code: (i) −x + 1, y + 1/2, −z + 3/2.

Oxygen atom O(1), involved in the interaction, is a little more distant from S(1) than the other oxygen atom O3 (1.438(2) vs. 1.430(2) Å), which is expected and confirms the hydrogen bonding. The chains are packed in the crystal in such a way that allows ring stacking interactions between ring C1-C6 and its symmetry equivalent related by inversion centre at (½ ½ 1) and ring C9-C14 and its equivalent related by inversion centre at (1 1 1) (see Figure 5).

Figure 5. Ring stacking interactions, operating among the hydrogen-bonded chains. Symmetry codes #1: (1 − x, 1 − y, 2 − z); #2: (2 − x, 2 − y, 2 − z). Only few, selected molecules are shown.

2.2. Cytotoxic Activity

Compounds 19‒47 were evaluated in vitro for their effects on the viability of three human cancer cell lines: MCF-7 (breast cancer), HCT-116 (colon cancer) and HeLa (cervical cancer). Cytotoxic evaluations were performed in a 5-dose assay and data is reported as the percent growth of the treated cells. Results in Table 4 show the growth percent (GP) at two representative doses, i.e., 10 and 100 µM. The most active compound 27 belonged to the 1-naphthyl series (R¹ = 1-naphthyl) and showed outstanding anticancer activity at low and high concentrations against all tested cell lines, especially towards to the HeLa cell line (GP = 7% at 100 µM, GP = 22% at 10 µM).

Table 4. Cytotoxicity of compounds 19‒47 toward human cancer cell lines.

**Table 4.** Cytotoxicity of compounds 19‒47 toward human cancer cell lines.
Compd.	GP (%) ^a
	MCF-7		HeLa		HCT-116
	10 µM	100 µM	10 µM	100 µM	10 µM	100 µM
19	88 ± 2	87 ± 2	100 ± 2	76 ± 2	100 ± 2	92 ± 3
20	88 ± 3	82 ± 2	90 ± 4	71 ± 2	101 ± 2	93 ± 2
21	94 ± 3	84 ± 3	97 ± 2	92 ± 1	93 ± 6	84 ± 6
22	91 ± 4	77 ± 7	91 ± 6	65 ± 7	86 ± 7	68 ± 5
23	93 ± 2	77 ± 2	87 ± 4	89 ± 6	86 ± 6	75 ± 3
24	93 ± 5	91 ± 5	97 ± 1	91 ± 1	91 ± 6	76 ± 4
25	97 ± 5	87 ± 5	94 ± 3	79 ± 6	84 ± 5	81 ± 6
26	97 ± 2	93 ± 6	98 ± 2	91 ± 1	85 ± 6	92 ± 5
27	82 ± 5	44 ± 5	22 ± 2	7 ± 0.6	63 ± 4	15 ± 1
28	96 ± 4	82 ± 4	100 ± 2	92 ± 3	86 ± 8	71 ± 2
29	95 ± 4	81 ± 4	87 ± 2	87 ± 3	74 ± 0.4	79 ± 1
30	93 ± 7	81 ± 1	95 ± 5	91 ± 1	85 ± 4	82 ± 2
31	93 ± 6	85 ± 6	95 ± 2	95 ± 4	86 ± 7	70 ± 4
32	96 ± 2	89 ± 3	100 ± 2	92 ± 4	89 ± 7	86 ± 5
33	93 ± 8	71 ± 3	100 ± 2	93 ± 3	86 ± 5	83 ± 5
34	86 ± 7	62 ± 0.4	96 ± 4	95 ± 5	92 ± 6	69 ± 6
35	89 ± 7	82 ± 4	98 ± 5	92 ± 4	84 ± 7	76 ± 2
36	93 ± 4	73 ± 0.1	101 ± 0.1	95 ± 1	93 ± 7	72 ± 7
37	95 ± 3	72 ± 0.5	99 ± 1	91 ± 4	91 ± 5	85 ± 5
38	95 ± 3	81 ± 0.8	98 ± 4	95 ± 2	99 ± 7	83 ± 4
39	94 ± 9	65 ± 5	99 ± 1	72 ± 5	94 ± 2	65 ± 2
40	93 ± 4	86 ± 8	99 ± 0.5	98 ± 0.5	96 ± 4	89 ± 2
41	100 ± 1	61 ± 0.1	100 ± 1	86 ± 3	92 ± 3	69 ± 2
42	89 ± 3	79 ± 2	98 ± 2	99 ± 0.2	92 ± 1	90 ± 4
43	95 ±5	81 ± 6	101 ± 3	95 ± 5	89 ± 5	83 ± 5
44	92 ± 6	66 ± 4	98 ± 1	43 ± 3	93 ± 4	40 ± 4
45	100 ± 3	76 ± 1	97 ± 3	18 ± 1	92 ± 7	61 ± 2
46	96 ± 5	79 ± 4	100 ± 2	80 ± 3	89 ± 6	84 ± 2
47	92 ± 4	46 ± 5	100 ± 2	9 ± 0.8	91 ± 4	18 ± 2

^a Growth percent (GP) was measured at concentrations 1, 10, 25, 50 and 100 µM. Two representative doses were shown, i.e., 10 µM and 100 µM. Values are expressed as the mean ± SD of at least three independent experiments.

It was noted that changing R¹ = 1-naphthyl in active compound 27 to the corresponding 2-naphthyl isomer (compound 28) markedly decreased this activity. Another 1-naphthyl derivative 47 also stood out from the other compounds and demonstrated dilution-dependent good cytotoxic activity toward the three cell lines (9% ≤ GP ≤ 46% at 100 µM). The significance of the 1-naphthyl substituent for the anticancer activity emphasize the fact that, replacement of this group (R¹ = 1-naphthyl, 47) by phenyl reduced activity of compound (R¹ = phenyl, 46, 79% ≤ GP ≤ 84% at 100 µM).

We found that among N-acylsulfonamides with R¹ = phenyl the presence of R² = phenyl group provided good cytotoxicity (compound 45) toward the HeLa cells (GP = 18%) and replacement of phenyl substituent in this compound by an aliphatic group, i.e., 21 (R² = Me), 33 (R² = Et), 43 (R² = iPr) or 44 (R² = CH₂CH₂(c–C₆H₁₁)) caused decreases in the activity of 44 (GP = 43%) or the loss of activity (21, 33, 43) at a concentration of 100 µM.

For the most active compounds 27, 44, 45 and 47, the concentration required for 50% inhibition of cell viability IC₅₀ was calculated and compared with the reference drug cisplatin, and the results are given in Table 5.

Table 5. IC₅₀ values for compounds 27, 44, 45 and 47.

**Table 5.** IC₅₀ values for compounds 27, 44, 45 and 47.
Compd.	IC₅₀ (µM)
Compd.	MCF-7	HeLa	HCT-116
27	30 ± 1.2	6 ± 0.2	14 ± 0.5
44	170 ± 12	95 ± 3	91 ± 3
45	200 ± 16	75 ± 4	135 ± 7
47	96 ± 5	43 ± 2	77 ± 3
Cisplatin	3.0 ± 0.1	3.8 ± 0.2	2.2 ± 0.1

Despite our observations concerning mentioned above correlations between structure and activity, we decided to apply more objective methods of establishing the structure-activity relationships, such as QSAR methodology.

2.3. Quantitative Structure-Activity Relationships (QSARs) of Cytotoxic Activity

The exploration of the relationship between structure and cytotoxic activity can be carried out by QSAR study. Recently, pharmacophore-based approaches are considered as the most advanced and successful, however if the molecular target of a group of compounds is not known, traditional regression-based techniques can be applied.

Multiple linear regression technique (MLR) along with stepwise algorithm usually leads to a statistically significant equation, that can be interpret based on a values of selected descriptors. Many pitfalls and mistakes can be done during development of a QSAR model. Among them, overfitting is one of the most common, and may results in false interpretation of a QSAR model. The second crucial issue is to develop a model in a way that enables to interpret quantitative relationship in the view of chemical structure. In order to achieve informative and statistically significant relationship we carefully designed several QSAR models using three-dimensional structures developed in Gaussian software and activity data taken from three distinct cell lines. Only selected descriptors available in DRAGON package were used (Table 6). MLR gave three statistically significant equations, one for each cell line (Table 7). The comparison between experimentally observed and predicted GP values is visualized in Figure 6.

Figure 6. The comparison between growth percent (GP) values observed experimentally and predicted by QSAR models for: MCF-7 (A); HeLa (B) and HCT-116 (C) cell lines. Training and test compounds are shown by red and blue circles, respectively.

For the MCF-7 cell line, three descriptors were pointed out as significant for compounds’ activity. First of all the number of ring systems (NRS), with its positive coefficient shows that additional rings generally decrease the biological activity. CATS2D_06_DL shows a preferable topological distance (6 bonds) between lipophilic and H-bond donor centers and F07[C–C] supplements its performance indicating a preferred distance between C atoms (Figure S1, see Supplementary Data). QSAR model for HeLa cell line contains four molecular descriptors. It shows two beneficial features: frequency of C–S atom pair at topological distance 8 and positive charge at the S atom of the sulfonamide moiety. Also two features are shown to decrease activity: topological distance between H-bond donor and acceptor (seven bonds) and the longest geometric distance from the atom to any other atom in the molecule (molecule width) (Figure S1, see Supplementary Data).

Table 6. Molecular descriptors (ring, topological, charge descriptors and atom-centred fragments) calculated by Dragon (Talete, Milano, Italy, version 6.0).

**Table 6.** Molecular descriptors (ring, topological, charge descriptors and atom-centred fragments) calculated by Dragon (Talete, Milano, Italy, version 6.0).
Compd.	Ring	Topological								Atom-Centred	Charge
Compd.	NRS	CATS2D_06_DL	CATS2D_07_DA	CATS2D_03_LL	F07 [C–C]	F08 [C–S]	F05 [C–S]	B10 [C–S]	ECC	C-024	qpmax
19	1	2	0	6	6	0	3	0	135	2	2.849
20	1	2	3	5	5	0	3	0	144	2	2.861
21	2	2	0	10	13	1	4	0	201	7	2.843
22	2	2	0	10	15	2	5	0	263	6	2.844
23	2	2	0	10	14	1	4	0	283	6	2.844
24	2	2	0	11	13	1	4	0	228	6	2.844
25	2	2	0	9	13	1	4	1	257	6	2.843
26	2	2	0	7	11	0	3	0	201	4	2.844
27	2	2	0	19	20	3	6	0	260	9	2.845
28	2	2	0	19	17	2	5	1	280	9	2.845
29	2	1	1	11	16	2	5	1	280	8	2.844
30	2	1	1	6	14	2	5	1	280	6	2.845
31	2	2	0	8	14	1	4	0	261	4	2.845
32	2	2	0	5	11	0	3	0	195	2	2.846
33	2	2	0	10	15	1	4	0	221	7	2.844
34	2	2	0	10	17	2	5	0	288	6	2.843
35	2	2	0	10	16	1	4	0	308	6	2.844
36	2	2	0	11	15	1	4	0	250	6	2.843
37	2	2	0	9	15	1	4	1	280	6	2.843
38	2	2	0	7	13	0	3	0	221	4	2.843
39	2	2	0	19	19	2	5	1	305	9	2.844
40	2	1	1	6	16	2	5	1	305	6	2.845
41	2	2	0	8	16	1	4	0	286	4	2.844
42	2	2	0	5	13	0	3	0	219	2	2.847
43	2	2	0	10	17	1	4	0	233	7	2.842
44	3	4	0	17	19	3	5	1	364	7	2.845
45	3	2	0	13	22	3	6	0	297	12	2.852
46	3	4	0	17	19	3	5	1	364	12	2.844
47	3	4	0	26	26	5	7	1	442	14	2.848

NRS—number of ring systems; CATS2D_06_DL—CATS2D Donor-Lipophilic at lag 06; F07[C–C]—frequency of C–C at topological distance 7; F08[C–S]—frequency of C–S at topological distance 8; qpmax—maximum positive charge (at sulfonamide S atom); CATS2D_07_DA—CATS2D Donor-Acceptor at lag 07; ECC—eccentricity (the longest geometric distance from the atom to any other atom in the molecule); CATS2D_03_LL—CATS2D Lipophilic-Lipophilic at lag 03; F05[C–S]—frequency of C–S at topological distance 5; C-024—number of fragments C_(Ar)–CH–C_(Ar); B10[C–S]—presence/absence of C–S at topological distance 10.

Table 7. Summary of the QSAR equations developed for compounds under the study.

**Table 7.** Summary of the QSAR equations developed for compounds under the study.
Cell Line: MCF-7
GP = 89.07 ± 6.24 + 36.51 ± 6.05NRS − 7.88 ± 2.51 CATS2D_06_DL − 4.81 ± 0.63 F07[C–C]
R² = 0.77; Q² = 0.59; F = 19.77; p = 6.3 × 10⁻⁶
p_NRS = 0.000011; p_{CATS22_06_DL} = 0.005632; p_F07[C–C] = 0.000001
Cell Line: HeLa
GP = 11409.30 ± 3252.32 − 24.10 ± 4.46 F08[C–S] − 3993.18 ± 1140.25 qpmax + 18.60 ± 5.92 CATS2D_07_DA + 0.24 ± 0.09 ECC
R² = 0.76; Q² = 0.81; F = 13,77; p = 3.5 × 10⁻⁵
p_F08[C–S] = 0.000048; p_qpmax = 0.002732; p_{CATS2D_07_DA} = 0.005922; p_ECC = 0.020743
Cell Line: HCT-116
GP = 146.53 ± 7.22 − 4.32 ± 0.76 CATS2D_03_LL − 15.90 ± 2.51 F05[C–S] + 5.98 ± 1.20 C-024 + 9.59 ± 3.72 B10[C–S]
R² = 0.89; Q² = 0.83; F = 36.89; p = 6.08 × 10⁻⁸
p_{CATS2D_03_LL} = 0.000026; p_F05[C–S] = 0.000007; p_C-024 = 0.000114; p_B10[C–S] = 0.019460

R²—squared correlation coefficient for training set; Q²—squared correlation coefficient for test set; F—Fisher’s test; p—p-value for Fisher’s test for the whole equation and particular descriptors.

The HCT-116 QSAR model showed the best performance (R² = 0.89; Q² = 0.83) for both the training and test sets of compounds (Figure 6C). This points out the increase of biological activity with the increased number of lipophilic centers at topological distance 3 and the frequency of C–S atom pair at distance 5. On the other hand, C_(Ar)–CH–C_(Ar) fragment as well as presence of C–S at topological distance 10 were showed to be unfavorable (Figure S1, see Supplementary Data).

2.4. Metabolic Stability

Most small-molecule anticancer drugs are cytotoxic agents with a narrow therapeutic window. Moreover their activity can be influenced by significant inter- and intra-subject variability, which can lead to life-threatening consequences. The probability to develop a successful drug can be increased by assessments of drugs’ pharmacokinetic characteristics, i.e., absorption, distribution, metabolism and excretion properties, as early as possible during the preclinical stage. Among them metabolism is very important because it involves conversion of a drug candidate into a set of metabolites that differ in both activity and toxicity, leading to complex interactions within the human body. Metabolic stability can be assessed by incubation of a potent drug in a presence of liver microsomes and NADPH to give an insight to metabolic properties [34,35,36].

Selected compounds were submitted to in vitro metabolic stability study in the presence of human liver microsomes and NADPH (Table 8). Determination of metabolic half-time (t_1/2) was performed by LC-MS analysis. Metabolic stability in this set of compounds, expressed as t_1/2, depends on a type of substituent in R¹ and R² positions. The most liable derivatives were 42 and 44 with t_1/2 below 5 min. Among the most potent compounds 27 and 47, the first one was more stable with t_1/2 24.23 min., which is a moderate value in a studied set of compounds. Two compounds substituted with ethynyl (compound 19) and amide (compound 20) moieties were characterized by a high metabolic stability, exceeding 40 min.

Table 8. Results of metabolic stability study.

**Table 8.** Results of metabolic stability study.
Compd.	In Vitro Metabolic Half-Life t_1/2 (min)
19	41.50
20	>>60
21	14.29
27	24.23
28	68.61
30	17.67
31	6.00
32	12.30
42	<5
44	<5
45	21.06
46	13.20
47	20.20

Interestingly, big difference in t_1/2 value was observed between 28 and 27. The increased metabolic stability results from the shift of naphthyl substituent from 1- to 2-naphthyl position. In order to describe this phenomenon in more detailed way, we decided to use in silico approach. Recently, Zaretzki and co-workers developed on-line accessible tool for accurate prediction of xenobiotic sites of metabolism, called XenoSite [37]. Among available models, one is able to predict stability measured in a presence of human liver microsomes. Taking into account in silico results illustrated in Figure 7, the relation with experimental determination of metabolic stability is clear. Compound 27 has two more sites vulnerable for metabolic biotransformation than 28, one in naphthalene ring and in methylene linker.

Figure 7. Sites of metabolism predicted for 27 and 28 by XenoSite software [37]. Green color indicates more vulnerability to biotransformation than blue. Some significant differences are additionally pointed out.

Among the most potent compounds, 44 bears an ethylcyclohexyl moiety, which is probably the reason for its low stability. This indicates that this substituent should be excluded from further synthesis in this series of derivatives. On the other hand, although 2-naphtyl substitution is preferable for metabolic stability in comparison with a 1-naphtyl moiety, the high biological response observed for the latter one predisposes it to be a lead compound, as it shows a good balance between stability and cytotoxic properties.

3. Experimental Section

3.1. General Information

Melting points were uncorrected and measured using a Boetius PHMK apparatus (Veb Analytic, Dresden, Germany). IR spectra were measured on a Satellite FTIR spectrometer (Thermo Mattson, Madison, WI, USA) using KBr pellets; the absorption range was 400–4000 cm⁻¹. ¹H-NMR and ¹³C-NMR spectra were recorded on a Gemini 200 apparatus or a Unity Plus 500 apparatus (Varian, Palo Alto, CA, USA). Chemical shifts are expressed at δ values relative to Me₄Si (TMS) as an internal standard. Elemental analyses were determined on a 2400 Series II CHN Elemental Analyzer (Perkin Elmer, Shelton, CT, USA) and were in agreement with the theoretical values within a ±0.4% range. Thin-layer chromatography (TLC) was performed on Kieselgel 60 F254 plates (Merck, Darmstadt, Germany) and visualized by UV. Commercially unavailable substrates were obtained according to the following methods described previously: 3-amino-6-chloro-7-methyl-1,1-dioxo-1,4,2-benzodithiazine (1) [26] N-(4-chloro-2-mercapto-5-methylbenzenesulfonyl)cyanamide dipotassium salt (2) [26], N-(2-allylthio-4-chloro-5-methylbenzenesulfonyl)cyanamide potassium salt (3) [27], N-[4-chloro-5-methyl-2-(prop-2-ynylthio)benzenesulfonyl]cyanamide potassium salt (4) [27], N-(2-carbamoylthio-4-chloro-5-methylbenzenesulfonyl)cyanamide potassium salt (5) [27], N-(2-benzylthio-4-chloro-5-methyl-benzenesulfonyl)cyanamide potassium salt (6) [26], N-[4-chloro-5-methyl-2-(3-trifluoromethyl-benzylthio)benzenesulfonyl]cyanamide potassium salt (7) [22], N-[4-chloro-5-methyl-2-(4-trifluoro-methylbenzylthio)benzenesulfonyl]cyanamide potassium salt (8) [22], N-[4-chloro-2-(4-chloro-benzylthio)-5-methylbenzenesulfonyl]cyanamide potassium salt (9) [38], N-[4-chloro-5-methyl-2-(naphthalen-1-ylmethylthio)benzenesulfonyl]cyanamide potassium salt (12) [22], N-[4-chloro-2-(2,3-dihydrobenzo[b][1,4]dioxin-2-ylmethylthio)-5-methylbenzenesulfonyl]cyanamide potassium salt (15) [22], and N-[4-chloro-2-(6-chlorobenzo[d][1,3]dioxol-5-ylmethylthio)-5-methylbenzenesulfonyl] cyanamide potassium salt (16) [39].

3.2. Chemistry

3.2.1. Procedures for Preparation of Cyanamide Potassium Salts 10–11, 13, 14, 17

N-[4-Chloro-2-(4-methoxybenzylthio)-5-methylbenzenesulfonyl]cyanamide Potassium Salt (10). To a solution of 2 (2.032 g, 6 mmol) in water (20 mL) 4-methoxybenzyl chloride (0.916 mL, 6.6 mmol) was added and the resulting mixture was stirred at 0 °C for 4 h. A white solid was filtered off and crystallized from ethanol, giving the title compound 10 (2.400 g, 95%): mp 127‒130 °C; IR (KBr) 2953, 2833 (CH₃, CH₂), 2176 (C≡N), 1343, 1176 (SO₂) cm⁻¹; ¹H-NMR (500 MHz, DMSO-d₆) δ 2.29 (s, 3H, CH₃), 3.72 (s, 3H, OCH₃), 4.19 (s, 2H, SCH₂), 6.88 (d, J = 8.3 Hz, 2H, arom.), 7.34 (d, J = 8.3 Hz, 2H, arom.), 7.37 (s, 1H, H-3), 7.72 (s, 1H, H-6) ppm; ¹³C-NMR (125 MHz, DMSO-d₆) δ 19.66, 36.20, 55.76, 114.54, 117.94, 127.35, 128.58, 131.00, 131.19, 131.61, 136.37, 136.56, 140.86, 159.11 ppm; anal. C 45.26, H 3.20, N 6.25% calcd for C₁₆H₁₄ClKN₂O₃S₂, C 45.65, H 3.35, N 6.65%.

N-[4-Chloro-5-methyl-2-(pyridin-4-ylmethylthio)benzenesulfonyl]cyanamide Potassium Salt (11). The mixture of 4-(chloromethyl)pyridine (1.548 g, 9.45 mmol) and K₂CO₃ (1.242 g, 9.00 mmol) in methanol (45 mL) was stirred at room temperature for 10 min, followed by addition of dipotassium salt 2 (3.050 g, 9.00 mmol). The resulting slurry was stirred at 60 °C for 1 h. After completion of reaction ethyl ether (45 mL) was added and a precipitated solid (4.410 g) was filtered off. The pure product was extracted with boiling ethanol (180 mL) giving the title compound 11 (2.982 g, 76%): mp 162‒164 °C; IR (KBr) 2924, 2853 (CH₃, CH₂), 2175 (C≡N), 1342, 1140 (SO₂) cm⁻¹; ¹H-NMR (500 MHz, DMSO-d₆) δ 2.27 (s, 3H, CH₃), 4.32 (s, 2H, SCH₂), 7.35 (s, 1H, H-3), 7.44 (d, J = 5.9 Hz, 2H, pyridine), 7.73 (s, 1H, H-6), 8.48 (d, J = 5.9 Hz, 2H, pyridine); ¹³C-NMR (125 MHz, DMSO-d₆) δ 19.66, 35.35, 117.88, 124.79, 127.89, 131.29, 132.30, 135.04, 136.40, 141.47, 146.71, 150.32 ppm; anal. C 42.70, H 2.75, N 10.60% calcd for C₁₄H₁₁ClKN₃O₂S₂, C 42.90, H 2.83, N 10.72%.

N-[4-Chloro-5-methyl-2-(naphthalen-2-ylmethylthio)benzenesulfonyl]cyanamide Potassium Salt (13). To a solution of 2 (0.700 g, 2.06 mmol) in water (6 mL) 2-(chloromethyl)naphthalene (0.457 g, 2.06 mmol) was added and the resulting mixture was stirred at room temperature for 12 h. The solid was filtered off and crystallized from ethanol to afford 13 (0.753 g, 83%): mp 207‒209 °C; IR (KBr) 2921 (CH₃, CH₂), 2175 (C≡N), 1342, 1140 ( SO₂) cm⁻¹; ¹H-NMR (500 MHz, DMSO-d₆) δ 2.27 (s, 3H, CH₃), 4.44 (s, 2H, SCH₂), 7.45 (s, 1H, arom.), 7.47‒7.50 (m, 2H, arom.), 7.59 (d, 1H, arom.), 7.73 (s, 1H, H-3), 7.84‒7.89 (m, 3H, arom.), 7.95 (s, 1H, H-6); ¹³C-NMR (125 MHz, DMSO-d₆) δ 19.66, 36.94, 117.96, 126.61, 126.97, 127.64,128.07, 128.23, 128.28, 128.68, 131.23, 131.86, 132.82, 133.53, 134.69, 136.10, 136.38, 141.13 ppm; anal. C 51.45, H 3.11, N 6.12% calcd for C₁₉H₁₄ClKN₂O₂S₂, C 51.75, H 3.20, N 6.35%.

N-[4-Chloro-5-methyl-2-(quinolin-2-ylmethylthio)benzenesulfonyl]cyanamide Potassium Salt (14). The mixture of 2 (0.505 g, 1.50 mmol), 2-chloromethylquinoline (0.320 g, 1.50 mmol), K₂CO₃ (0.104 g, 0.75 mmol) in methanol (10 mL) was stirred at 50 °C for 2 h. The reaction mixture was evaporated under reduced pressure to dryness, then treated with water (10 mL) and stirred using an ultrasonic bath for 3 min. The obtained solid was filtered off and crystallized from ethanol to afford 14 (0.359 g, 54%): mp 207‒210 °C; IR (KBr) 2924 (CH₃CH₂), 2176, (C≡N), 1344, 1143 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 2.26 (s, 3H, CH₃), 4.54 (s, 2H, SCH₂), 7.50‒7.82 (m, 5H, H-6, H-3 and quinol.), 7.85‒8.20 (m, 2H, quinol.), 8.35 (d, 1H, quinol.); ¹³C-NMR (125 MHz, DMSO-d₆) δ 19.56, 39.27, 117.88, 122.24, 127.15, 127.39, 127.55, 128.52, 129.03, 130.47, 131.17, 131.77, 135.89, 136.39, 137.63, 140.89, 147.38, 158.54 ppm; anal. C 47.00, H 2.80, N 9.00% calcd for C₁₈H₁₃ClKN₃O₂S₂, C 47.20, H 2.86, N 9.17%.

N-[4-Chloro-2-(3,5-dimethylisoxazol-4-ylmethylthio)-5-methylbenzenesulfonyl]cyanamide Potassium Salt (17). To the mixture of 2 (0.500 g, 1.48 mmol) in water (4 mL) chloromethyl-3,5-dimethylisoxazol (0.183 mL, 1.48 mmol) was added and stirred at room temperature for 1.5 h, then at 100 °C for 6 h. After standing overnight at room temperature a precipitated solid was filtered off and crystallized from ethanol to afford the title compound 17 (0.700 g, 58%): mp 205‒206 °C; IR (KBr) 2923, 2853 (CH₃, CH₂), 2181 (C≡N), 1274, 1141 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 2.25 (s, 3H, CH₃), 2.33 (s, 3H, CH₃), 2.34 (s, 3H, CH₃), 4.09 (s, 2H, SCH₂), 7.44 (s, 1H, H-3), 7.75 (s, 1H, H-6) ppm; ¹³C-NMR (50 MHz, DMSO-d₆) δ 9.99, 10.85, 19.29, 24.76, 109.41, 117.46, 129.13, 130.77, 132.30, 134.51, 135.79, 141.94, 159.86, 166.79 ppm; anal. C 40.72, H 3.15, N 10.05% calcd for C₁₄H₁₃ClKN₃O₃S₂, C 41.02, H 3.20, N 10.25%.

3.2.2. Procedure for the Preparation of N-Acylbenzenesulfonamides 18‒44

A solution of monopotassium salts 3‒17 (1 mmol) in an appropriate carboxylic acid (40 mmol) was refluxed for 1‒17 h and left overnight at room temperature. The precipitate was filtered off, dried and crystallized from ethanol.

N-(2-Allylthio-4-chloro-5-methylphenylsulfonyl)acetamide (18). Starting from 3 (0.341 g) and acetic acid (2.5 mL) the title compound 18 (0.230 g, 72%) was obtained after 15 h at reflux: mp 138‒140 °C; IR (KBr) 3334, 3241 (NH), 2923 (CH₃, CH₂), 1686 (C=O), 1352, 1160 (SO₂) cm⁻¹; ¹H-NMR (500 MHz, DMSO-d₆) δ 1.95 (s, 3H, CH₃CO), 2.40 (s, 3H, CH₃), 3.86 (d, 2H, SCH₂), 4.94 (dd, 2H, =CH₂), 5.77–5.84 (m, 1H, HC=), 7.62 (s, 1H, H-3), 7.95 (s, 1H, H-6), 12.34 (s, 1H, NH) ppm; anal. C 44.75, H 4.31, N 4.61% calcd for C₁₂H₁₄ClNO₃S₂, C 45.06, H 4.41, N 4.38%.

N-[4-Chloro-5-methyl-2-(prop-2-ynylthio)phenylsulfonyl]acetamide (19). Starting from 4 (0.339 g) and acetic acid (2.5 mL) the title compound 19 was obtained (0.113 g, 35%) after for 17 h at reflux: mp 148‒150 °C; IR (KBr) 3448, 3298 (NH), 3086 (C≡CH), 2981, 2876 (CH₃, CH₂), 1692 (C=O), 1472 (N–H), 1353, 1165 (SO₂) cm⁻¹; ¹H-NMR (500 MHz, DMSO-d₆) δ 1.91 (s, 3H, CH₃CO), 2.36 (s, 3H, CH₃), 3.20 (s, 1H, C≡CH), 4.06 (s, 2H, SCH₂), 7.68 (s, 1H, H-3), 7.92 (s, 1H, H-6), 12.34 (s, 1H, NH); ¹³C-NMR (125 MHz, DMSO-d₆) δ 19.69, 21.00, 23.90, 74.95, 80.03, 129.01, 133.66, 134.19, 135.11, 135.82, 139.56, 169.41 ppm; anal. C 44.96, H 3.75, N 4.08% calcd for C₁₂H₁₂ClNO₃S₂, C 45.35, H 3.81, N 4.41%.

N-(4-Chloro-2-carbamoylmethylthio-5-methylphenylsuflonyl)acetamide (20). Starting from 5 (0.358 g) and acetic acid (2.5 mL) the title compound 20 was obtained (0.101 g, 60%) after 4.5 h at reflux, followed by additional cooling in an ice-water bath for 3 h: mp 220‒225 °C; IR (KBr) 3466, 3355 (NH), 2860, 2701 (CH₃, CH₂), 1693 (C=O), 1476, 1457 (N–H), 1160, 1119 (SO₂) cm⁻¹; ¹H-NMR (500 MHz, DMSO-d₆) δ 1.93 (s, 3H, CH₃CO), 2.34 (s, 3H, CH₃), 3.81 (s, 2H, SCH₂), 7.27 (s, 1H, CONH₂), 7.60 (s, 1H, CONH₂), 7.62 (s, 1H, H-3), 7.89 (s, 1H, H-6), 12.30 (s, 1H, NH); ¹³C-NMR (125 MHz, DMSO-d₆) δ 19.63, 23.83, 36.95, 128.79, 133.27, 134.16, 135.21, 136.77, 139.63, 169.42, 169.68 ppm; anal. C 38.84, H 3.78, N 7.95% calcd for C₁₁H₁₃ClN₂O₄S₂, C 39.23, H 3.89, N 8.32%.

N-(2-Benzylthio-4-chloro-5-methylphenylsulfonyl)acetamide (21). Starting from 6 (0.400 g) and acetic acid (2.5 mL) the title compound 21 was obtained (0.200 g, 54%) after 2 h at reflux, followed by additional cooling in an ice-box for 12 h: mp 155‒158 °C; IR (KBr) 3116 (N–H), 2921 (CH₃, CH₂), 1686 (C=O), 1454 (N–H), 1356, 1165 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 1.92 (s, 3H, CH₃CO), 2.35 (s, 3H, CH₃), 4.45 (s, 2H, SCH₂), 7.26‒7.48 (m, 5H, arom.), 7.67 (s, 1H, H-3), 7.90 (s, 1H, H-6), 12.36 (s, 1H, NH); ¹³C-NMR (125 MHz, DMSO-d₆) δ 19.64, 23.76, 36.77, 128.14, 128.73, 129.18, 129.81, 133.08, 134.16, 135.13, 136.62, 139.59, 169.26 ppm; anal. C 51.89, H 4.35, N 3.77% calcd for C₁₆H₁₆ClNO₃S₂, C 51.95, H 4.36, N 3.79%.

N-(4-Chloro-5-methyl-2-(3-trifluoromethylbenzylthio)phenylsulfonyl)acetamide (22). Starting from 7 (0.459 g) and acetic acid (2.5 mL) the title compound 22 was obtained (0.367 g, 84%) after 2 h at reflux, followed by additional cooling an ice-box for 12 h: mp 126‒130 °C; IR (KBr) 3221 (N–H), 3065 (C–H arom.), 2986, 2855 (CH_3, CH₂), 1721 (C=O), 1448 (N–H), 1330, 1164 ( SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 1.92 (s, 3H, CH₃CO), 2.35 (s, 3H, CH₃), 4.58 (s, 2H, SCH₂),7.56‒7.82 (m, 5H, H-3, arom.), 7.91 (s, 1H, H-6), 12.37 (s, 1H, NH) ppm; ¹³C-NMR (50 MHz, DMSO-d₆) δ 19.22, 23.24, 35.66, 124.43, 124.51, 126.06, 126.13, 128.76, 129.10, 129.73, 129.87, 133.12, 133.36, 133.80, 135.11, 135.32, 138.11, 139.21, 168.82 ppm; anal. C 46.49, H 3.42, N 3.13% calcd for C₁₇H₁₅ClF₃NO₃S₂, C 46.63, H 3.45, N 3.20%.

N-(4-Chloro-5-methyl-2-(4-trifluoromethylbenzylthio)phenylsulfonyl)acetamide (23). Starting from 8 (0.459 g) and acetic acid (2.5 mL), the title compound 23 was obtained (0.219 g, 50%) after 1.5 h at reflux, followed by additional cooling in an ice-box for 12 h: mp 180‒181 °C; IR (KBr) 3443 (N–H), 3062 (C–H arom.), 2878, 2710 (CH₃, CH₂), 1687 (C=O), 1482 (N–H), 1321, 1160 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 1.91 (s, 3H, CH₃CO), 2.36 (s, 3H, CH₃), 4.56 (s, 2H, SCH₂), 7.63‒7.75 (m, 5H, H-3, arom.), 7.91 (s, 1H, H-6), 12.34 (s, 1H, NH) ppm; anal. C 46.66, H 3.45, N 3.21% calcd for C₁₇H₁₅ClF₃NO₃S₂, C 46.63, H 3.45, N 3.20%.

N-[4-Chloro-2-(4-chlorobenzylthio)-5-methylphenylsulfonyl]acetamide (24). Starting from 9 (0.338 g) and acetic acid (2.5 mL), the title compound 24 was obtained (0.219 g, 50%) after 2 h at reflux, followed by additional cooling in an ice-box for 12 h: mp 182‒184 °C; IR (KBr) 3445 (N–H), 3052 (C–H arom.), 2869, 2711 (CH₃, CH₂), 1683 (C=O), 1490 (N–H), 1350, 1162 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 1.92 (s, 3H, CH₃CO), 2.35 (s, 3H, CH₃), 4.46 (s, 2H, CH₂S), 7.42 (d, 2H, arom.), 7.47 (d, 2H, arom.), 7.68 (s, 1H, H-3), 7.81 (s, 1H, H-6), 12.35 (s, 1H, NH); ¹³C-NMR (125 MHz, DMSO-d₆) δ 19.65, 23.75, 35.97, 128.95, 129.16, 131.57, 132.74, 133.34, 134.18, 135.32, 135.93, 136.12, 139.62, 169.28 ppm; anal. C 47.26, H 3.69, N 3.33% calcd for C₁₆H₁₅ClNO₃S₂, C 47.53, H 3.74, N 3.46%.

N-[4-Chloro-2-(4-methoxybenzylthio)-5-methylphenylsulfonyl]acetamide (25). Starting from 10 (0.421 g) and acetic acid (2.5 mL) the crude product was obtained after refluxing for 1 h, then evaporating the mixture under reduced pressure. Crystallization from ethanol gave the title compound 25 (0.204 g, 51%): mp 82‒84 °C; IR (KBr) 3427 (N–H), 2924, 2853 (CH₃, CH₂), 1705 (C=O), 1450 (N–H), 1303, 1032 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 1.79 (s, 3H, CH₃CO), 2.32 (s, 3H, CH₃), 3.74 (s, 3H, CH₃O), 4.25 (s, 2H, CH₂S), 6.90 (d, 2H, arom.), 7.35 (d, 2H, arom.), 7.45 (s, 1H, H-3), 7.81 (s, 1H, H-6), 12.20 (br s, 1H, NH) ppm; anal. C 51.20, H 4.57, N 3.58% calcd for C₁₇H₁₈ClNO₄S₂, C 51.06, H 4.54, N 3.50%.

N-[4-Chloro-2-(4-pyridinylmethylthio)-5-methylphenylsulfonyl]acetamide (26). Starting from 11 (0.392 g) and acetic acid (2.5 mL) the crude product was obtained after 1.5 h at reflux, then evaporating the mixture under reduced pressure. Crystallization from ethanol/chloroform (v/v = 2:1) gave the title compound 26 (0.167 g, 45%): mp 179‒183 °C; IR (KBr) 3413 (N–H), 2923, 2852 (CH₃, CH₂), 1711 (C=O), 1492 (N–H), 1325, 1153 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 1.92 (s, 3H, CH₃CO), 2.34 (s, 3H, CH₃), 4.49 (s, 2H, CH₂S), 7.44 (d, 2H, pyridine), 7.67 (s, 1H, H-3), 7.90 (s, 1H, H-6), 8.52 (d, 2H, pyridine), 12.40 (s, 1H, NH) ppm; anal. C 48.39, H 4.00, N 7.45% calcd for C₁₅H₁₅ClN₂O₃S₂, C 48.58, H 4.08, N 7.55%.

N-[4-Chloro-5-methyl-2-(naphth-1-ylmethyl)phenylsulfonyl]acetamide (27). Starting from 12 (0.442 g) and acetic acid (2.5 mL) a white solid was obtained after refluxing for 1.5 h, followed by additional cooling in an ice-box for 12 h. The product was crystallized from acetonitrile to afford the title compound 27 (0.227 g, 54%): mp 176‒178 °C; IR (KBr) 3438 (N–H), 3061 (C–H arom), 2921, 2852 (CH₃, CH₂), 1727 (C=O), 1443 (N–H), 1352, 1155 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 1.92 (s, 3H, CH₃CO), 2.39 (s, 3H, CH₃), 4.91 (s, 2H, CH₂S), 7.45‒7.66 (m, 4H, arom.), 7.77 (s, 1H, H-3) 7.89‒8.00 (m, 3H, H-6 and arom.), 8.25 (d, 1H, arom.), 12.20 (s, 1H, NH) ppm; anal. C 57.23, H 4.32, N 3.33% calcd for C₂₀H₁₈ClNO₃S₂, C 57.20, H 4.32, N 3.34%.

N-[4-Chloro-5-methyl-2-(naphth-2-ylmethyl)phenylsulfonyl]acetamide (28). Starting from 13 (0.442 g) and acetic acid (2.5 mL) the title compound 28 was obtained (0.210 g, 50%) after 2 h at reflux, followed by additional cooling in an ice-box for 12 h: mp 138‒141 °C; IR (KBr) 3423 (N–H); 3066 (C–H arom.); 2919, 2870 (CH₃, CH₂), 1683 (C=O), 1353, 1161 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 1.93 (s, 3H, CH₃CO), 2.33 (s, 3H, CH₃), 4.63 (s, 2H, CH₂S), 7.40‒7.60 (m, 3H, arom.), 7.75 (s, 1H, H-3), 7.80‒7.96 (m, 5H, H-6 and arom.), 12.36 (s, 1H, NH) ppm; anal. C 57.30, H 4.30, N 3.39% calcd for C₂₀H₁₈ClNO₃S₂, C 57.20, H 4.32, N 3.34%.

N-[4-Chloro-5-methyl-2-(quinolin-2-yl)phenylsulfonyl]acetamide (29). Starting from 14 (0.421 g) and acetic acid (2.5 mL), the title compound 29 was obtained (0.219 g, 52%) after refluxing with stirring for 2 h, followed by additional cooling in an ice-box for 12 h: mp 153.8‒157 °C; IR (KBr) 3421 (N–H), 3069 (C–H arom.), 2922, 2851 (CH₃, CH₂), 1719 (C=O), 1457 (N–H), 1351, 1157 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 1.91 (s, 3H, CH₃CO), 2.32 (s, 3H, CH₃), 3.36 (s, 2H, CH₂S), 7.60 (t, 1H, quinol.), 7.68 (s, 1H, H-3), 7.79 (t, 1H, quinol.), 7.88 (s, 1H, H-6), 7.90‒8.00 (m, 3H, quinol.), 8.37 (d, 1H, quinol.), 12.40 (s, 1H, NH) ppm; anal. C 54.60, H 3.92, N 6.37% calcd for C₁₉H₁₇ClN₂O₃S₂, C 54.21, H 4.07, N 6.66%.

N-[4-Chloro-2-(2,3-dihydrobenzo[b][1,4]dioxin-2-ylmethylthio)-5-methylphenylsulfonyl]acetamide (30). Starting from 15 (0.449 g) and acetic acid (2.5 mL), the title compound 30 was obtained (0.235 g, 55%) after refluxing for 1 h 15 min, followed by additional cooling in an ice-box for 12 h: mp 188‒191.5 °C; IR (KBr) 3268 (N–H), 3067 (C–H arom.), 2985, 2883 (CH₃, CH₂), 1722 (C=O), 1495 (N–H), 1329, 1159 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 1.92 (s, 3H, CH₃CO), 2.38 (s, 3H, CH₃), 3.50 (d, 2H, CH₂O), 4.14 (dd, 1H, CHO), 4.38 (d, 2H, CH₂S), 6.66–6.89 (m, 4H, arom.), 7.74 (s, 1H, H-3), 7.94 (s, 1H, H-6), 12.41 (s, 1H, NH) ppm; anal. C 50.50, H 4.24, N 3.28% calcd for C₁₈H₁₈ClNO₅S₂, C 50.52, H 4.24, N 3.27%.

N-[4-Chloro-2-(6-chlorobenzo[d][1,3]dioxol-5-ylmethylthio)-5-methylphenylsulfonyl]acetamide (31). Starting from 16 (0.469 g) and acetic acid (2.5 mL) the crude product was obtained after 1.5 h at reflux, followed by additional cooling in an ice-box for 12 h,. Crystallization from ethanol/chloroform (v/v = 2.7:1) gave the title compound 31 (0.264 g, 59%): mp 122‒124 °C; IR (KBr) 3424 (N–H), 2922, 2852 (CH₃, CH₂), 1719 (C=O), 1478 (N–H), 1251, 1159 (SO₂) cm⁻¹; ¹H NMR (200 MHz, DMSO-d₆) δ 1.81 (s, 3H, CH₃CO), 2.34 (s, 3H, CH₃), 4.30 (s, 2H, CH₂S), 6.08 (s, 2H, CH₂O), 7,12 (s, 1H, arom.), 7.13 (s, 1H, arom.), 7.44 (s, 1H, H-3), 7.85 (s, 1H, H-6), 12.40 (s, 1H, NH) ppm; anal. C 45.71, H 3.32, N 3.03% calcd for C₁₇H₁₅C_l2NO₅S₂, C 45.54, H 3.37, N 3.12%.

N-[4-Chloro-2-(3,5-dimethylisoxazol-4-ylmethylthio)-5-methylphenylsulfonyl]acetamide (32). Starting from 17 (0.338 g) and acetic acid (2.5 mL), a product was obtained after 1.5 h at reflux, followed by additional cooling in an ice-box for 12 h. Crystallization from 80% ethanol gave the title compound 32 (0.241 g, 62%): mp 154‒160 °C; IR (KBr) 3421 (N–H), 3086 (C–H arom.), 2922, 2853 (CH₃, CH₂), 1711 (C=O), 1455 (N–H), 1351, 1161 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 1.92 (s, 3H, CH₃CO), 2.26 (s, 3H, CH₃), 2.37 (s, 3H, CH₃), 2.39 (s, 3H, CH₃), 4.28 (s, 2H, CH₂S), 7.73 (s, 1H, H-3), 7.93 (s, 1H, H-6), 12.32 (s, 1H, NH) ppm; anal. C 45.95, H 4.25, N 6.88% calcd for C₁₅H₁₇ClN₂O₄S₂, C 46.33, H 4.41, N 7.20%.

N-(2-Benzylthio-4-chloro-5-methylphenylsulfonyl)propionamide (33). Starting from 6 (0.385 g) and propionic acid (3.0 mL) the product was obtained after 1 h at reflux, then evaporating the mixture under reduced pressure and treating the residue with water (10 mL). Crystallization of the crude product from ethyl acetate/petroleum ether gave the title compound 33 (0.330 g, 86%): mp 137‒139 °C; IR (KBr) 3440 (N–H), 3033 (C–H arom.), 2922, 2874 (CH₃, CH₂), 1673 (C=O), 1469 (N–H), 1355, 1162 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 0.89 (t, 3H, CH₃CH₂CO), 2.23 (q, 2H, CH₂CO), 2,36 (s, 3H, CH₃), 4.44 (s, 2H, CH₂S), 7.24‒7.45 (m, 5H, arom.), 7.67 (s, 1H, H-3), 7.92 (s, 1H, H-6), 12.31 (s, 1H, NH); ¹³C-NMR (125 MHz, DMSO-d₆) δ 8.94, 19.65, 29.23, 36.73, 128.13, 128.65, 129.17, 129.78, 133.08, 134.19, 135.17, 136.57, 136.62, 139.57, 172.77 ppm; anal. C 53.39, H 4.83, N 3.90% calcd for C₁₇H₁₈ClNO₃S₂, C 53.18, H 4.73, N 3.65%.

N-[4-Chloro-5-methyl-2-(3-(trifluoromethylbenzylthio)phenylsulfonyl]propionamide (34). Starting from 7 (0.459 g) and propionic acid (3.0 mL) the product was obtained after refluxing for 2 h, then evaporating the mixture under reduced pressure and treating of residue with water (10 mL). Crystallization from 80% ethanol gave the title compound 34 (0.307 g, 68%): mp 123‒127 °C; IR (KBr) 3242 (N–H), 2926, 2853 (CH₃, CH₂), 1726 (C=O), 1431 (N–H), 1326, 1128 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 0.87 (t, 3H, CH₃CH₂CO), 2.27 (q, 2H, CH₂CO), 2.35 (s, 3H, CH₃), 4.56 (s, 2H, CH₂S), 7.60‒7.75 (m, 4H, H-3, arom.), 7.80 (m, 1H, arom.), 7.91 (s, 1H, H-6), 12.32 (s, 1H, NH) ppm; anal. C 47.94, H 3.82, N 3.15% calcd for C₁₈H₁₇ClF₃NO₃S₂, C 47.84, H 3.79, N 3.10%.

N-[4-Chloro-5-methyl-2-(4-trifluoromethylbenzylthio)phenylsulfonyl]propionamide (35). Starting from 8 (0.451 g) and propionic acid (3.0 mL) the product was obtained after refluxing for 1 h, then evaporating the mixture under reduced pressure and treating of residue with water (10 mL). Crystallization from ethyl acetate/petroleum ether gave the title compound 35 (0.420 g, 93%): mp 107‒108 °C; IR (KBr) 3218 (N–H), 2988, 2952 (CH₃, CH₂), 1734 (C=O), 1325, 1122 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 0.86 (t, 3H, CH₃CH₂CO), 2.19 (q, 2H, CH₂CO), 2.36 (s, 3H, CH₃), 4.56 (s, 2H, CH₂S), 7.66‒7.72 (m, 5H, H-3, arom.), 7.92 (s, 1H, H-6), 12.31 (s, 1H, NH) ppm; anal. C 47.96, H 3.81, N 3.16% calcd for C₁₈H₁₇ClF₃NO₃S₂, C47.84, H 3.79, N 3.10%.

N-[4-Chloro-2-(4-chlorobenzylthio)-5-methylphenylsulfonyl]propionamide (36). Starting from 9 (0.338 g) and propionic acid (3.0 mL) the product was obtained after refluxing for 2 h, then evaporating the mixture under reduced pressure and treating of residue with water (10 mL). Crystallization from 96% ethanol gave the title compound 36 (0.284 g, 68%): mp 87‒91 °C; IR (KBr) 3116 (N–H), 2927, 2865 (CH₃, CH₂), 1719 (C=O), 1456 (N–H), 1319, 1132 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 0.88 (t, 3H, CH₃CH₂CO), 2,26 (q, 2H, CH₂CO), 2,36 (s, 3H, CH₃), 4,44 (s, 2H, CH₂S), 7,36 (d, 2H, arom.), 7.44 (d, 2H, arom.), 7.67 (s, 1H, H-3), 7.91 (s, 1H, H-6), 12.27 (s, 1H, NH) ppm; anal. C 48.98, H 4.15, N 3.46% calcd for C₁₇H₁₇Cl₂NO₃S₂, C 48.81, H 4.10, N 3.35%.

N-[4-Chloro-2-(4-methoxybenzylthio)-5-methylphenylsulfonyl]propionamide (37). Starting from 10 (0.421 g) and propionic acid (3.0 mL) the product was obtained after refluxing for 1 h, then evaporating the mixture under reduced pressure and treating of residue with water (10 mL). Crystallization from benzene gave the title compound 37 (0.352 g, 85%): mp 130‒132 °C; IR (KBr) 3268 (N–H), 3032 (C–H arom.), 2935, 2853 (CH₃, CH₂), 1732 (C=O), 1441 (N–H), 1349, 1175 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 0.89 (t, 3H, CH₃CH₂CO), 2.22 (q, 2H, CH₂CO), 2.36 (s, 3H, CH₃), 3.73 (s, 3H, OCH₃), 4.37 (s, 2H, CH₂S), 6.89 (d, 2H, arom.), 7.34 (d, 2H, arom.), 7.66 (s, 1H, H-3), 7.91 (s, 1H, H-6), 12.27 (s, 1H, NH); ¹³C-NMR (125 MHz, DMSO-d₆) δ 8.92, 19.65, 29.22, 36.29, 55.75, 114.56, 128.20, 128.65, 129.00, 130.99, 132.97, 134.15, 135.13, 136.79, 139.55, 159.27, 172.75 ppm; anal. C 52.11, H 4.85, N 3.32% calcd for C₁₈H₂₀ClNO₄S₂, C 52.23, H 4.87, N 3.38%.

N-[4-Chloro-5-methyl-2-(pyridin-4-ylmethylthio)phenylsulfonyl]propionamide (38). Starting from 11 (0.392 g) and propionic acid (3.0 mL) the product was obtained after refluxing for 2 h 15 min, then evaporating the mixture under reduced pressure and treating of residue with water (10 mL). Crystallization from ethanol gave the title compound 38 (0.250 g, 65%): mp 151‒154 °C; IR (KBr) 3422 (N–H), 2925, 2852 (CH₃, CH₂), 1715 (C=O), 1349, 1150 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 0.88 (t, 3H, CH₃CH₂CO), 2.24 (q, 2H, CH₂CO), 2.35 (s, 3H, CH₃), 4.50 (s, 2H, CH₂S), 7.44 (d, 2H, pyridine), 7.68 (s, 1H, H-3), 7.92 (s, 1H, H-6), 8.51 (d, 2H, pyridine), 12.38 (s, 1H, NH); ¹³C-NMR (125 MHz, DMSO-d₆) δ 8.91, 19.66, 29.25, 35.25, 124.69, 128.87, 133.57, 134.29, 135.46, 135.59, 139.63, 146.34, 150.35, 172.80 ppm; anal. C 50.02, H 4.47, N 7.32% calcd for C₁₆H₁₇ClN₂O₃S₂, C 49.93, H 4.45, N 7.28%.

N-[4-Chloro-5-methyl-2-(naphthalen-2-ylmethylthio)phenylsulfonyl]propionamide (39). Starting from 13 (0.441 g) and propionic acid (3.0 mL) the product was obtained after refluxing for 1.5 h, then evaporating the mixture under reduced pressure and treating of residue with water (10 mL). Crystallization from 80% ethanol gave the title compound 39 (0.373 g, 86%): mp 85‒88 °C; IR (KBr) 3207 (N–H), 3056 (C–H arom.), 2920, 2855 (CH₃, CH₂), 1731 (C=O), 1446 (N–H), 1321, 1176 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 0.84 (t, 3H, CH₃CH₂CO), 2.24 (q, 2H, CH₂CO), 2.33 (s, 3H, CH₃), 4.62 (s, 2H, CH₂S), 7.49‒7.60 (m, 4H, arom.), 7.75 (s, 1H, H-3), 7.83‒7.94 (m, 4H, H-6, arom.), 12.30 (s, 1H, NH) ppm; anal. C 58.48, H 4.80, N 3.55% calcd for C₂₁H₂₀ClNO₃S₂, C 58.12, H 4.65, N 3.23%.

N-[4-Chloro-2-(2,3-dihydrobenzo[b][1,4]dioxin-2-ylmethylthio)-5-mehylphenylsulfonyl]propionamide (40). Starting from 15 (0.449 g) and propionic acid (3.0 mL) the product was obtained after refluxing with stirring for 1 h 45 min, then evaporating the mixture under reduced pressure and treating of residue with water (10 mL). Crystallization from ethanol gave the title compound 40 (0.384 g, 87%): mp 114‒116.2 °C; IR (KBr) 3224 (N–H), 2920, 2852 (CH₃, CH₂), 1730 (C=O), 1495 (N–H), 1270, 1136 (SO₂) cm⁻¹; ¹H-NMR (500 MHz, DMSO-d₆) δ 0.83 (t, 3H, CH₃CH₂CO), 2.14 (q, 2H, CH₂CO), 2.34 (s, 3H, CH₃), 3.36‒3.47 (m, 2H, CH₂O), 4.06 (dd, 1H, CHO), 4.32 (d, 2H, CH₂S), 6.70‒6.85 (d, 4H, arom.), 7.63 (s, 1H, H-3), 7.88 (s, 1H, H-6), 12.26 (s, 1H, NH) ppm; ¹³C-NMR (50 MHz, DMSO-d₆) δ 9.11, 19.31, 29.95, 33.28, 66.23, 72.08, 117.17, 117.29, 121.66, 121.79, 128.82, 132.73, 133.23, 135.02, 137.93, 142.57, 142.98, 174.53 ppm; anal. C 51.73, H 4.58, N 3.22% calcd for C₁₉H₂₀ClNO₅S₂, C 51.64, H 4.56, N 3.17%.

N-[4-Chloro-2-(6-chlorobenzo[d][1,3]dioxol-5-ylmethylthio)-5-methylphenylsulfonyl]propionamide (41). Starting from 16 (0.469 g) and propionic acid (3.0 mL) the product was obtained after refluxing for 1 h, then evaporating the mixture under reduced pressure and treating of residue with water (10 mL). Crystallization from ethyl acetate/petroleum ether gave the title compound 41 (0.376 g, 81%): mp 142‒144 °C; IR (KBr) 3205 (N–H), 2922, 2898 (CH₃, CH₂), 1686 (C=O), 1474 (N–H), 1250, 1116 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 0.88 (t, 3H, CH₃CH₂CO), 2.23 (q, 2H, CH₂CO), 2.38 (s, 3H, CH₃), 4.39 (s, 2H, CH₂S), 6.08 (s, 2H, CH₂), 7.11 (s, 2H, arom.), 7.62 (s, 1H, H-3), 7.93 (s, 1H, H-6), 12.26 (s, 1H, NH); ¹³C-NMR (125 MHz, DMSO-d₆) δ 8.87, 19.68, 29.20, 35.34, 102.90, 110.33, 111.13, 126.15, 126.89, 128.80, 133.38, 134.23, 135.33, 136.46, 139.66, 147.32, 148.48, 172.80 ppm; anal. C 46.70, H 3.70, N 3.01% calcd for C₁₈H₁₇ClNO₅S₂, C 46.76, H 3.71, N 3.03%.

N-[4-Chloro-2-(3,5-dimethylisoxazol-4-ylmethylthio)-5-methylphenylsulfonyl]propionamide (42). Starting from 17 (0.339 g) and propionic acid (3.0 mL) the product was obtained after refluxing for 1.5 h, then evaporating the mixture under reduced pressure and treating of residue with water (10 mL). Crystallization from ethanol/chloroform (v/v = 2:1) gave the title compound 42 (0.374 g, 68%): mp 160‒163 °C; IR (KBr) 3433 (N–H), 3064 (C–H arom.), 2935, 2859 (CH₂, CH₂), 1726 (C=O), 1455 (N–H), 1353, 1132 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 0.89 (t, 3H, CH₃CH₂CO), 2.17‒2.25 (m, 5H, CH₂CO and CH₃), 2.37 (s, 3H, CH₃), 2.39 (s, 3H, CH₃), 4.28 (s, 2H, CH₂S), 7.71 (s, 1H, H-3), 7.94 (s, 1H, H-6), 12.28 (s, 1H, NH); ¹³C-NMR (125 MHz, DMSO-d₆) δ 8.91, 10.31, 11.29, 19.69, 25.35, 29.15, 109.37, 129.65, 133.61, 134.18, 135.78, 136.14, 139.57, 160.22, 167.67, 172.78 ppm; anal. C 47.75, H 4.75, N 6.97% calcd for C₁₆H₁₉ClN₂O₄S₂ C 47.70, H 4.75, N 6.95%.

N-(2-Benzylthio-4-chloro-5-methylphenylsulfonyl)isobutyramide (43). Starting from 6 (0.391 g) and isobutyric acid (3.7 mL) the product was obtained after refluxing for 2 h, then dropping water (10 mL) into the cooled reaction mixture. Crystallization from ethanol gave the title compound 43 (0.378 g, 95%): mp 122‒125 °C; IR (KBr) 3423 (N–H), 2926, 2868 (CH₃, CH₂), 1694 (C=O), 1433 (N–H), 1351, 1172 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 0.96 (d, 6H, CH₃), 2.36 (s, 3H, CH₃), 2.40‒2.60 (m, 1H, CH); 4.43 (s, 2H, CH₂S), 7.20‒7.45 (m, 5H, arom.), 7.64 (s, 1H, H-3), 7.92 (s, 1H, H-6), 12.28 (s, 1H, NH) ppm; ¹³C-NMR (50 MHz, DMSO-d₆) δ 24.14, 24.66, 39.81, 41.90, 133.18, 133.45, 134.23, 134.84, 138.00, 139.31, 140.03, 141.41, 141.81, 144.59, 180.90 ppm; anal. C 54.39, H 5.07, N 3.55% calcd for C₁₈H₂₀ClNO₃S₂, C 54.33, H 5.07, N 3.52%.

N-(2-Benzylthio-4-chloro-5-methylphenylsulfonyl)-3-cyclohexylpropionamide (44). Starting from 6 (0.338 g) and 3-cyclohexenepropionic acid (6.3 mL) the crude product was obtained after refluxing for 1.5 h at 115 °C. Crystallization from acetonitrile gave the title compound 44 (0.378 g, 81%): mp 118‒120 °C; IR (KBr) 3427 (N–H), 3060 (C–H arom.), 2923, 2840 (CH₃, CH₂), 1678 (C=O), 1448 (N–H), 1359, 1171 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 0.8‒0.98 (m, 2H, cHex.), 1.10‒1.20 (m, 4H, cHex.), 1.40 (q, 2H, CH₂), 1.50‒1,80 (m, 5H, cHex.), 2.22 (t, 2H, CH₂CO), 2.36 (s, 3H, CH₃), 4.43 (s, 2H, CH₂S), 7.20‒7.45 (m, 5H, arom.), 7.64 (s, 1H, H-3), 7.92 (s, 1H, H-6), 12.29 (s, 1H, NH) ppm; anal. C 59.19, H 6.04, N 2.97% calcd for C₂₃H₂₈ClNO₃S₂, C 59.27, H 6.06, N 3.01%.

N-(2-Benzylthio-4-chloro-5-methylphenylsulfonyl)benzamide (45). A mixture of 6 (0.391 g, 1 mmol) and benzoic acid (0.244 g, 2 mmol) in water (4 mL) was stirred in a pressure tube at 100 °C for 144 h. The product was isolated from the hot reaction mixture by filtration. After crystallization from 90% ethanol the title compound 45 was obtained (0.311 g, 72%): mp 168‒170 °C; IR (KBr): 3274 (N–H), 3064 (C–H arom.), 2918 (CH₃, CH₂), 1693 (C=O), 1453 (N–H), 1332, 1163 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 2.34 (s, 3H, CH₃), 4.40 (s, 2H, CH₂S), 7.10‒7.20 (m, 3H, arom.), 7.24‒7.36 (m, 2H, arom.), 7.45‒7.58 (m, 2H, arom.), 7.60‒7.72 (m, 2H, H-3 and arom.), 7.88‒7.98 (d, 2H, arom.), 8.02 (s, 1H, H-6), 12.82 (s, 1H, NH); ¹³C-NMR (125 MHz, DMSO-d₆) δ 19.69, 36.65, 128.02, 128.66, 129.03, 129.23, 129.65, 131.98, 133.12, 133.98, 134.44, 135.16, 136.40, 136.66, 139.68, 165.86 ppm; anal. C 58.19, H 4.15, N 3.15% calcd for C₂₁H₁₈ClNO₃S₂, C 58.39, H 4.20, N 3.24%.

N-(2-Benzylthio-4-chloro-5-methylphenylsulfonyl)-3-phenylacrylamide (46). A mixture of 6 (0.391 g, 1 mmol) and cinnamic acid (0.296 g, 2 mmol) in water (6 mL) was stirred in a pressure tube at 100 °C for 96 h. The product was isolated from the hot reaction mixture by filtration. After crystallization from 80% ethanol the title compound 46 was obtained (0.348 g, 76%): mp 173‒174 °C; IR (KBr) 3199 (N–H), 2919, 2854 (CH₃, CH₂), 1675 (C=O), 1439 (N–H), 1353, 1131 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 2.37 (s, 3H, CH₃), 4.43 (s, 2H, CH₂S), 6.74 (d, 1H, CH=), 7.16‒7.24 (m, 3H, arom.), 7.34‒7.68 (m, 9H, arom.), 7.99 (s, 1H, H-6), 12.54 (s, 1H, NH) ppm; ¹³C-NMR (50 MHz, DMSO-d₆) δ 19.25, 36.37, 118.99, 127.59, 128.42, 128.49, 128.62, 129.31, 129.43, 131.04, 132.77, 133.97, 134.13, 134.79, 136.08, 139.26, 144.23, 163.43 ppm; anal. C 60.30, H 4.40, N 3.05% calcd for C₂₃H₂₀ClNO₃S₂, C 60.32, H 4.40, N 3.06%.

N-[4-Chloro-5-methyl-2-(naphthalen-1-ylmethylthio)phenylsulfonyl]-3-phenylacrylamide (47). A mixture of 12 (0.450 g, 1 mmol) and cinnamic acid (0.148 g, 1.1 mmol) in toluene (5 mL) was stirred in a pressure tube at 110‒120 °C for 120 h. A solvent was evaporated under reduced pressure to dryness and residue was treated with water (150 mL) and stirred using an ultrasonic bath for 15 min. Product was isolated by extraction with chloroform (3 × 50 mL), then the combined organic layers were washed with 1% NaHCO₃ (2 × 50 mL), 1 M HCl (50 mL), water (2 × 50 mL) and dried with MgSO₄. The solvent was evaporated under reduced pressure and residue was purified by crystallization from ethanol giving the titled compound 47 (0.254 g, 50%): mp 105‒106 °C; IR (KBr) 3192 (N–H), 2923, 2853 (CH₃, CH₂), 1683 (C=O), 1448 (N–H), 1351, 1178 (SO₂) cm⁻¹; ¹H-NMR (200 MHz, DMSO-d₆) δ 2.42 (s, 3H, CH₃), 4.90 (s, 2H, CH₂S), 6.58 (d, 1H, CH=), 7.35‒7.64 (m, 10H, CH= and arom.), 7.77 (s, 1H, H-3), 7.82‒7.94 (m, 2H, arom.), 8.03 (s, 1H, H-6), 8.18 (d, 1H, arom.), 12.40 (s, 1H, NH); ¹³C-NMR (125 MHz, DMSO-d₆) δ 19.70, 35.22, 119.30, 124.81, 126.09, 126.57, 127.00, 128.79, 128.90, 129.09, 129.15, 129.79, 131.47, 131.72, 132.01, 133.13, 134.08, 134.32, 134.48, 134.83, 137.35, 139.81, 144.59, 163.87 ppm; anal. C 63.70, H 4.33, N 2.69% calcd for C₂₇H₂₂ClNO₃S₂, C 63.83, H 4.36, N 2.76%.

3.3. X-ray Structure Determination

Experimental diffraction data were collected on a KM4 CCD kappa-geometry diffractometer (Oxford Diffraction, Yarnton, Oxfordshire, UK), equipped with a Sapphire2 CCD detector. An enhanced X-ray Mo Ka radiation source with a graphite monochromator was used. Determination of the unit cell and diffraction data collection were carried out at room temperature (298 K). All calculations (data reduction, structure solution, and refinement) were carried out using CrysAlisPro package [40]. The structure was solved by direct methods, and all nonhydrogen atoms were refined with anisotropic thermal parameters by full-matrix least squares procedure based on F². Final refinements were carried out using the SHELX-97 package [41], run under control of WinGX program [42]. Scattering power of all the crystals tested was low, so in spite of long frame exposure time (240 s), the ratio of observed to unique reflections is only 40%.

Crystallographic data for structure of 22 reported in this paper have been deposited with the Cambridge Crystallographic Data Center as supplementary publication No. CCDC 1400372. Copies of the data can be obtained free of charge on application to CCDC, 12 Union Road, Cambridge CB2 1EZ, UK (Fax: +44-1223-336-033; Email: [email protected]).

3.4. Cell Culture and Cell Viability Assay

All chemicals, if not stated otherwise, were obtained from Sigma-Aldrich (St. Louis, MO, USA). The MCF-7 cell line was purchased from Cell Lines Services (Eppelheim, Germany), the HeLa and HCT-116 cell lines were obtained from the Department of Microbiology, Tumor and Cell Biology, Karolinska Institute (Stockholm, Sweden). Cells were cultured in in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum, 2 mM glutamine, 100 units/mL penicillin, and 100 μg/mL streptomycin. Cultures were maintained in a humidified atmosphere with 5% CO₂ at 37 °C in an incubator (HeraCell, Heraeus, Langenselbold, Germany).

Cell viability was determined using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide) assay. Cells were seeded in 96-well plates at a density of 5 ×10³ cells/well and treated for 72 h with the examined compounds in the concentration range 1–100 μM (1, 10, 25, 50 and 100 μM). Following treatment, MTT (0.5 mg/mL) was added to the medium and cells were further incubated for 2 h at 37 °C. Cells were lysed with DMSO and the absorbance of the formazan solution was measured at 550 nm with a plate reader (1420 multilabel counter, Victor, Jügesheim, Germany). The optical density of the formazan solution was measured at 550 nm with a plate reader (1420 multilabel counter, Victor, Germany). The experiment was performed in triplicate. Values are expressed as the mean ± SD of at least three independent experiments.

3.5. Molecular Modeling Methodology/Calculations

The two-dimensional structure of the 29 studied compounds were built using the graphical user interface of Gaussian software (Gaussian 03W, v 6.0, Gaussian Inc., Wallingford, CT, USA), before starting molecular modeling algorithms, in order to obtain the lowest energy geometry of a molecule. The resulting structures were optimized by means of a semi-empirical method (with the use of the AM1 Hamiltonian). The developed three-dimensional structures were submitted to descriptor calculations using Dragon software. Among over four thousand descriptors available, only selected blocks were calculated: Constitutional Descriptors, Ring Descriptors, Charge descriptors, Topological indices, Geometrical descriptors, Functional group counts, Atom centered fragments, Atom-type E-state indices, CASTS2D descriptors, 2D atom pairs, Molecular properties and subsequently used in the QSAR study.

3.6. QSAR Study

Multiple linear regression with stepwise algorithm was performed and evaluated in Statistica software (Statsoft, Tulsa, OK, USA). 473 descriptors were used as a candidate for independent variables and percent of growth (GP) was used as a dependent variable. Dataset was randomly divided into training (n = 22, 75% of compounds) and test set (n = 7, 25% of compounds).

3.7. Metabolic Stability

Stock solutions of studied compounds were prepared at concentration of 10 mM in DMSO. Working solutions were prepared by dilution of stock with reaction buffer or acetonitrile, final concentration of organic solvent did not exceed 1%. Incubation mixture contained 10 μM of a studied derivative, 1 mM of NADPH (Sigma-Aldrich) and 0.5 mg/mL of human liver microsomes (HLM, Sigma-Aldrich) in potassium phosphate buffer (0.1 M, pH 7.4). Incubation was carried out in thermostat at 37 °C and started by addition of compound of interest. 50 μL samples were taken after 5, 15, 30, 45 and 60 min. Enzymatic reaction was terminated by the addition of the equal volume of ice-cold acetonitrile containing 10 μM of internal standard (IS). Compound 47 served as IS for compound 19. Compound 19 served as IS for the remaining compounds. Control incubations were performed without NADPH to assess chemical instability. After collection, samples were immediately centrifuged (10 min, 10,000 rpm) and resulted supernatant was directly analyzed or kept in −80 °C until LC-MS analysis. Natural logarithm of a compound over IS peak area ratio was plotted vs. incubation time. Metabolic half-time (t_1/2) was calculated from the slope of the linear regression, as reported in [43].

LC-MS analysis was performed on an Agilent 1260 system coupled to SingleQuad 6120 mass spectrometer (Agilent Technologies, Santa Clara, CA, USA). Cortex C18+ column (2.1 × 75 mm, 2.7 μm, Waters, Milford, MA, USA) was used in reversed-phase mode with gradient elution starting with 30% of phase A (10 mM ammonium formate in water) and 70% of phase B (10 mM ammonium formate in acetonitrile-water mixture, 95:5 v/v). The amount of phase B was linearly increased to 100% in 12 min. Total analysis time was 20 min at 40 °C, flow rate was 0.25 mL/min and the injection volume was 10 μL. The mass spectrometer was equipped with electrospray ion source and operated in positive ionization. Mass analyzer was set individually to each compound to detect [M + H]⁺ pseudomolecular ions. MSD parameters of the ESI source were as follows: nebulizer pressure 50 psig (N₂), drying gas 13 mL/min (N₂), drying gas temperature 300 °C, capillary voltage 3.5 kV, fragmentor voltage 100 V.

4. Conclusions

We have developed a new and facile method for the synthesis of N-acylated sulfonamides using N-(benzenesulfonyl)cyanamide potassium salts and carboxylic acids. The molecular structures of novel compounds were confirmed by NMR, IR, and also by single crystal X-ray structure analysis for representative compound 22. The newly synthesized N-acylsulfonamides were tested for their in vitro cytotoxic activity against the MCF-7, HCT-116 and HeLa cell lines. The most active compounds belonged to a series of 1-naphthyl derivatives. Compound 27 showed outstanding anticancer activity at low and high concentrations against all tested cell lines, especially with regard to the HeLa cell line (GP = 7% at 100 µM, GP = 22% at 10 µM). Compound 47 demonstrated dependent on dilution good cytotoxic activity toward three cell lines (9% ≤ GP ≤ 46% at 100 µM). Performed QSAR analysis demonstrate the importance of some molecular descriptors in the cytotoxic activities against the investigated cell lines. The MCF-7 and HCT-116 QSAR models showed that antitumor activity of N-acylsulfonamides depends on distinct topological descriptors and number of fragments C_(Ar)–CH–C_(Ar). On the other hand, QSAR analysis indicated the significance of topological descriptors as well as positive charge at sulfonamide S atom in the prediction of cytotoxic activity against HeLa cell line. Finally, the QSAR studies revealed three predictive and statistically significant models for the investigated compounds, with squared correlation coefficients for training set R² = 0.76‒0.89 and squared correlation coefficients for test set Q² = 0.59‒0.83. In vitro tests for metabolic stability in the presence of human liver microsomes and NADPH run on selected compounds showed that chemical nature of both R¹ and R² substituents of N-acylsulfonamides affected their stability. In the studied series of N-acylbenzenesulfonamides MLR-based considerations on structure-activity relationships served as a supporting approach beneficial for explanation of biological response. The usage of easily interpretable molecular descriptors is a base to plan further, rational synthesis of potentially more potent derivatives.

Supplementary Materials

Supplementary materials can be accessed at: https://www.mdpi.com/1420-3049/20/10/19101/s1.

Acknowledgments

This project was financed by National Science Centre based on the decision number DEC-2013/09/B/NZ7/00048.

Author Contributions

B.Ż. and J.S. created the concept, and designed the study. J.S. and B.Ż. performed chemical research and analyzed both the chemical and biological data. K.S. and A.P. synthesized some of the final compounds. B.Ż. and J.S. wrote the manuscript together. J.C. performed crystallographic analysis. A.K. tested the biological activity of the compounds and interpreted the results. M.B. and T.B. performed statistical analysis and interpreted the statistical models. All authors read and approved the final version of the article.

Conflicts of Interest

The authors declare no conflict of interest.

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Sample Availability: Samples of the compounds 1–47 are available from the authors.

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Żołnowska, B.; Sławiński, J.; Belka, M.; Bączek, T.; Kawiak, A.; Chojnacki, J.; Pogorzelska, A.; Szafrański, K. Synthesis, Molecular Structure, Metabolic Stability and QSAR Studies of a Novel Series of Anticancer N-Acylbenzenesulfonamides. Molecules 2015, 20, 19101-19129. https://doi.org/10.3390/molecules201019101

AMA Style

Żołnowska B, Sławiński J, Belka M, Bączek T, Kawiak A, Chojnacki J, Pogorzelska A, Szafrański K. Synthesis, Molecular Structure, Metabolic Stability and QSAR Studies of a Novel Series of Anticancer N-Acylbenzenesulfonamides. Molecules. 2015; 20(10):19101-19129. https://doi.org/10.3390/molecules201019101

Chicago/Turabian Style

Żołnowska, Beata, Jarosław Sławiński, Mariusz Belka, Tomasz Bączek, Anna Kawiak, Jarosław Chojnacki, Aneta Pogorzelska, and Krzysztof Szafrański. 2015. "Synthesis, Molecular Structure, Metabolic Stability and QSAR Studies of a Novel Series of Anticancer N-Acylbenzenesulfonamides" Molecules 20, no. 10: 19101-19129. https://doi.org/10.3390/molecules201019101

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Synthesis, Molecular Structure, Metabolic Stability and QSAR Studies of a Novel Series of Anticancer N-Acylbenzenesulfonamides

Abstract

1. Introduction

2. Results and Discussion

2.1. Chemistry

2.2. Cytotoxic Activity

2.3. Quantitative Structure-Activity Relationships (QSARs) of Cytotoxic Activity

2.4. Metabolic Stability

3. Experimental Section

3.1. General Information

3.2. Chemistry

3.2.1. Procedures for Preparation of Cyanamide Potassium Salts 10–11, 13, 14, 17

3.2.2. Procedure for the Preparation of N-Acylbenzenesulfonamides 18‒44

3.3. X-ray Structure Determination

3.4. Cell Culture and Cell Viability Assay

3.5. Molecular Modeling Methodology/Calculations

3.6. QSAR Study

3.7. Metabolic Stability

4. Conclusions

Supplementary Materials

Acknowledgments

Author Contributions

Conflicts of Interest

References

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