Identification and Expression Analysis of Zebrafish (Danio rerio) E-Selectin during Embryonic Development
AbstractIn this study, we cloned the full-length cDNA of E-selectin of zebrafish (Danio rerio), analyzed its expression pattern and preliminarily explored its biological function. Zebrafish E-selectin cDNA is 3146 bp and encodes a putative 871 amino acid protein. All structural domains involved in E-selectin function are conserved in the putative protein. Whole-mount in situ hybridization of zebrafish at 24 and 48 h post-fertilization (hpf) revealed E-selectin expression mainly in vascular/endothelial progenitor cells in the posterior trunk and blood cells in the intermediate cell mass and posterior cardinal vein regions. Real-time quantitative RT-PCR analysis detected E-selectin expression at 0.2, 24 and 48 hpf and significantly decreased from 48 to 72 hpf. The expression of E-selectin, tumor necrosis factor-α and interleukin-1β was significantly upregulated at 22 to 72 h after induction with bacterial lipopolysaccharide. Thus, the structure of E-selectin protein is highly conserved among species, and E-selectin may be involved in embryonic development and essential for hematopoiesis and angiogenesis during embryonic development in zebrafish. Furthermore, we provide the first evidence of inflammatory mediators inducing E-selectin expression in non-mammalian vertebrates, which suggests that zebrafish E-selectin may be involved in inflammation and probably has similar biological function to mammalian E-selectin. View Full-Text
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Sun, G.; Liu, K.; Wang, X.; Liu, X.; He, Q.; Hsiao, C.-D. Identification and Expression Analysis of Zebrafish (Danio rerio) E-Selectin during Embryonic Development. Molecules 2015, 20, 18539-18550.
Sun G, Liu K, Wang X, Liu X, He Q, Hsiao C-D. Identification and Expression Analysis of Zebrafish (Danio rerio) E-Selectin during Embryonic Development. Molecules. 2015; 20(10):18539-18550.Chicago/Turabian Style
Sun, Guijin; Liu, Kechun; Wang, Xue; Liu, Xiuhe; He, Qiuxia; Hsiao, Chung-Der. 2015. "Identification and Expression Analysis of Zebrafish (Danio rerio) E-Selectin during Embryonic Development." Molecules 20, no. 10: 18539-18550.