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Molecules 2014, 19(9), 14710-14722;

Chitosan Combined with Molecular Beacon for Mir-155 Detection and Imaging in Lung Cancer

Cancer Institute of PLA, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
Department of Oncology, People's Liberation Army 532 Hospital, Huangshan 242700, China
Diagnosis and Treatment Center of Cancer, Chengdu Military General Hospital, Chengdu 646000, China
Authors to whom correspondence should be addressed.
Received: 7 July 2014 / Revised: 30 August 2014 / Accepted: 3 September 2014 / Published: 16 September 2014
(This article belongs to the Section Medicinal Chemistry)
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Lung cancer is the major cause of cancer-related deaths worldwide, thus developing effective methods for its early diagnosis is urgently needed. In recent years, microRNAs (miRNAs, miR) have been reported to play important roles in carcinogenesis and have become potential biomarkers for cancer diagnosis and treatment. Molecular beacon (MB) technology is a universal technology to detect DNA/RNA expression in living cells. As a natural polymers, chitosan (CS) nanoparticles could be used as a carrier for safe delivery of nucleic acid. In this study, we developed a probe using nanoparticles of miR-155 MB self assembled with CS (CS-miR-155 MB) to image the expression of miR-155 in cancer cells. Hybridization assay showed that the locked nucleic acid (LAN) modified miR-155 MB could target miR-155 effectively and sensitively. The miR-155 MB self-assembly with CS nanoparticles formed stable complexes at the proper weight ratio. The CS nanoparticles showed higher fluorescence intensity and transfection efficiency than the lipid-based formulation transfection agent by confocal microscopy and flow cytometry analysis. The CS-MB complexes were found to be easily synthesized and exhibited strong enzymatic stability, efficient cellular uptake, high target selectivity and biocompatibility. The CS-MB complexes can also be applied in other cancers just by simply changing for a targeted miRNA highly expressed in those cancer cells. Therefore, it is a promising vehicle used for detecting miRNA expression in living cells. View Full-Text
Keywords: lung cancer; microRNA; molecular beacon; chitosan; nanoparticles; molecular imaging lung cancer; microRNA; molecular beacon; chitosan; nanoparticles; molecular imaging

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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Zhu, H.-Z.; An, J.-H.; Yao, Q.; Han, J.; Li, X.-T.; Jiang, F.-L.; Chen, G.-P.; Peng, L.-N.; Li, Y.-S.; Sun, J.-G.; Chen, Z.-T. Chitosan Combined with Molecular Beacon for Mir-155 Detection and Imaging in Lung Cancer. Molecules 2014, 19, 14710-14722.

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