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Molecules 2014, 19(9), 13432-13447;

Immunomodulatory Activity and Partial Characterisation of Polysaccharides from Momordica charantia

State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, China
Sericulture and Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China
University of Chinese Academy of Science, Beijing 100049, China
Author to whom correspondence should be addressed.
Received: 23 July 2014 / Revised: 27 August 2014 / Accepted: 27 August 2014 / Published: 29 August 2014
(This article belongs to the Section Natural Products Chemistry)
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Momordica charantia Linn. is used as an edible and medicinal vegetable in sub-tropical areas. Until now, studies on its composition and related activities have been confined to compounds of low molecular mass, and no data have been reported concerning the plant’s polysaccharides. In this work, a crude polysaccharide of M. charantia (MCP) fruit was isolated by hot water extraction and then purified using DEAE-52 cellulose anion-exchange chromatography to produce two main fractions MCP1 and MCP2. The immunomodulatory effects and physicochemical characteristics of these fractions were investigated in vitro and in vivo. The results showed that intragastric administration of 150 or 300 mg·kg·d−1 of MCP significantly increased the carbolic particle clearance index, serum haemolysin production, spleen index, thymus index and NK cell cytotoxicity to normal control levels in cyclophosphamide (Cy)-induced immunosuppressed mice. Both MCP1 and MCP2 effectively stimulated normal and concanavalin A-induced splenic lymphocyte proliferation in vitro at various doses. The average molecular weights of MCP1 and MCP2, which were measured using high-performance gel permeation chromatography, were 8.55 × 104 Da and 4.41 × 105 Da, respectively. Both fractions exhibited characteristic polysaccharide bands in their Fourier transform infrared spectrum. MCP1 is mainly composed of glucose and galactose, and MCP2 is mainly composed of glucose, mannose and galactose. The results indicate that MCP and its fractions have good potential as immunotherapeutic adjuvants. View Full-Text
Keywords: Momordica charantia; polysaccharide; immunomodulatory activity; structure Momordica charantia; polysaccharide; immunomodulatory activity; structure

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Deng, Y.-Y.; Yi, Y.; Zhang, L.-F.; Zhang, R.-F.; Zhang, Y.; Wei, Z.-C.; Tang, X.-J.; Zhang, M.-W. Immunomodulatory Activity and Partial Characterisation of Polysaccharides from Momordica charantia. Molecules 2014, 19, 13432-13447.

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