Next Article in Journal
Cucurbitane Glycosides Derived from Mogroside IIE: Structure-Taste Relationships, Antioxidant Activity, and Acute Toxicity
Previous Article in Journal
Ultrasound-Assisted Extraction of Antioxidants in Misai Kucing (Orthosiphon stamineus)
Article Menu

Export Article

Open AccessArticle
Molecules 2014, 19(8), 12660-12675;

Prebiotic Potential of Agave angustifolia Haw Fructans with Different Degrees of Polymerization

Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional, P.O. Box 24, Yautepec 62730, Morelos, Mexico
Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana-Xochimilco, Calzada del Hueso 1100, Col. Villa Quietud, Delegación Coyoacán 04960, D.F., Mexico
Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Col. Santo Tomas, Delegación Miguel Hidalgo 11340, D.F., Mexico
Authors to whom correspondence should be addressed.
Received: 13 June 2014 / Revised: 14 July 2014 / Accepted: 5 August 2014 / Published: 19 August 2014
(This article belongs to the Section Metabolites)
Full-Text   |   PDF [1897 KB, uploaded 19 August 2014]   |  


Inulin-type fructans are the most studied prebiotic compounds because of their broad range of health benefits. In particular, plants of the Agave genus are rich in fructans. Agave-derived fructans have a branched structure with both β-(2→1) and β-(2→6) linked fructosyl chains attached to the sucrose start unit with a degree of polymerization (DP) of up to 80 fructose units. The objective of this work was to assess the prebiotic potential of three Agave angustifolia Haw fructan fractions (AFF) with different degrees of polymerization. The three fructan fractions were extracted from the agave stem by lixiviation and then purified by ultrafiltration and ion exchange chromatography: AFF1, AFF2 and AFF3 with high (3–60 fructose units), medium (2–40) and low (2–22) DP, respectively. The fructan profile was determined with high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), which confirmed a branched fructan structure. Structural elucidation was performed by Fourier Transform Infra-Red Spectroscopy. The AFF spectrum shows characteristic fructan bands. The prebiotic effect of these fractions was assessed in vitro through fermentation by Bifidobacterium and Lactobacillus strains. Four growth patterns were observed. Some bacteria did not grow with any of the AFF, while other strains grew with only AFF3. Some bacteria grew according to the molecular weight of the AFF and some grew indistinctly with the three fructan fractions. View Full-Text
Keywords: Bifidobacterium; Lactobacillus; Agave angustifolia Haw; prebiotic; fructans Bifidobacterium; Lactobacillus; Agave angustifolia Haw; prebiotic; fructans

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Velázquez-Martínez, J.R.; González-Cervantes, R.M.; Hernández-Gallegos, M.A.; Mendiola, R.C.; Aparicio, A.R.J.; Ocampo, M.L.A. Prebiotic Potential of Agave angustifolia Haw Fructans with Different Degrees of Polymerization. Molecules 2014, 19, 12660-12675.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top