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Molecules 2014, 19(7), 9535-9551; https://doi.org/10.3390/molecules19079535

Secondary Metabolite Profiling of Curcuma Species Grown at Different Locations Using GC/TOF and UPLC/Q-TOF MS

1
Integrated Metabolomics Research Group, Western Seoul Center, Korea Basic Science Institute, Seoul 120-140, Korea
2
Department of Chemistry, Sungkyunkwan University, Suwon 440-746, Korea
3
Basic Herbal Medicine Research Group, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea
4
Herbal Material Management Team, Herbal Medicine Research Division, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea
5
Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 305-764, Korea
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 16 April 2014 / Revised: 26 June 2014 / Accepted: 30 June 2014 / Published: 4 July 2014
(This article belongs to the Section Metabolites)
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Abstract

Curcuma, a genus of rhizomatous herbaceous species, has been used as a spice, traditional medicine, and natural dye. In this study, the metabolite profile of Curcuma extracts was determined using gas chromatography-time of flight mass spectrometry (GC/TOF MS) and ultrahigh-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) to characterize differences between Curcuma aromatica and Curcuma longa grown on the Jeju-do or Jin-do islands, South Korea. Previous studies have performed primary metabolite profiling of Curcuma species grown in different regions using NMR-based metabolomics. This study focused on profiling of secondary metabolites from the hexane extract of Curcuma species. Principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA) plots showed significant differences between the C. aromatica and C. longa metabolite profiles, whereas geographical location had little effect. A t-test was performed to identify statistically significant metabolites, such as terpenoids. Additionally, targeted profiling using UPLC/Q-TOF MS showed that the concentration of curcuminoids differed depending on the plant origin. Based on these results, a combination of GC- and LC-MS allowed us to analyze curcuminoids and terpenoids, the typical bioactive compounds of Curcuma, which can be used to discriminate Curcuma samples according to species or geographical origin. View Full-Text
Keywords: Curcuma aromatica; Curcuma longa; Zngiberaceae; metabolite profiling; secondary metabolites; GC/TOF MS; UPLC/Q-TOF MS Curcuma aromatica; Curcuma longa; Zngiberaceae; metabolite profiling; secondary metabolites; GC/TOF MS; UPLC/Q-TOF MS
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Lee, J.; Jung, Y.; Shin, J.-H.; Kim, H.K.; Moon, B.C.; Ryu, D.H.; Hwang, G.-S. Secondary Metabolite Profiling of Curcuma Species Grown at Different Locations Using GC/TOF and UPLC/Q-TOF MS. Molecules 2014, 19, 9535-9551.

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