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Molecules 2014, 19(6), 8212-8219; https://doi.org/10.3390/molecules19068212

Inhibitory Activity of the Flower Buds of Lonicera japonica Thunb. against Histamine Production and L-Histidine Decarboxylase in Human Keratinocytes

1
Fundamental Research Laboratories, Hoyu Co., Ltd., Roboku 1-12, Nagakute City Aichi 480-1136, Japan
2
Kampo Research Laboratories, Kracie Pharma., Ltd., Kanebo machi 3-1, Takaoka City Toyama 933-0856, Japan
*
Author to whom correspondence should be addressed.
Received: 8 May 2014 / Revised: 4 June 2014 / Accepted: 10 June 2014 / Published: 17 June 2014
(This article belongs to the Section Natural Products Chemistry)
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Abstract

In previous studies we found that anionic surfactants such as sodium laurate (SL) and/or sodium dodecylsulfate (SDS) exert actions on epidermal keratinocytes rather than mast cells to give rise of histamine production and skin itching through increasing the expression of the 53-kDa active form of l-histidine decarboxylase (HDC). In addition, with treatment of SL in a three-dimensional human keratinocyte culture, increases in both the 53-kDa HDC and histamine production are detected and thus this culture assay is applied to screen anti-itching materials from natural resources. In this study, the inhibitory activity of “Kin-gin-ka” (flower buds of Lonicera japonica Thunb., FLJ) against histamine production and expression of the active form of HDC were examined in this culture assay. FLJ is a well-known traditional Chinese medicine, being used to treat fevers, coughs and some infectious diseases. The result showed both FLJ and chlorogenic acid had inhibitory activities against the expression of 53-kDa HDC and histamine production. However, chlorogenic acid showed a weaker effect on histamine production than that of FLJ, suggesting that other chemical constituents besides chlorogenic acid could contribute to the inhibitory activities. Thus, a further chemical study of FLJ is now under investigation. View Full-Text
Keywords: flower buds of Lonicera japonica Thunb.; chlorogenic acid; histamine; L-histidine decarboxylase; human keratinocytes flower buds of Lonicera japonica Thunb.; chlorogenic acid; histamine; L-histidine decarboxylase; human keratinocytes
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Inami, Y.; Matsui, Y.; Hoshino, T.; Murayama, C.; Norimoto, H. Inhibitory Activity of the Flower Buds of Lonicera japonica Thunb. against Histamine Production and L-Histidine Decarboxylase in Human Keratinocytes. Molecules 2014, 19, 8212-8219.

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