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Open AccessArticle

Isolation and Characterization of a Novel Lectin from the Edible Mushroom Stropharia rugosoannulata

State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China
Institute of Biotechnology and Germplasmic Resource, Yunnan Academy of Agricultural Science, Kunming 650223, China
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
College of Food Science and Technology, Nanjing Agricultural University, Weigang, Nanjing 210095, China
Author to whom correspondence should be addressed.
Molecules 2014, 19(12), 19880-19891;
Received: 14 October 2014 / Revised: 18 November 2014 / Accepted: 24 November 2014 / Published: 28 November 2014
(This article belongs to the Special Issue Lectins)
To date, only a few steroids have been isolated from the mushroom Stropharia rugosoannulata which can be cultivated. In this paper, a novel lectin (SRL) with a molecular weight of 38 kDa, and a unique IKSGVYRIVSWQGALGPEAR N-terminal sequence was isolated from S. rugosoannulata, which represents the first protein isolated from the mushroom. The purification methods included (NH4)2SO4 precipitation, ion exchange chromatography on CM-cellulose, Q-Sepharose, and SP-Sepharose, and gel- filtration on Superdex-75. The lectin was adsorbed on all three types of ion exchangers and was purified more than 450-fold. The lectin was stable below 70 °C (with half of the activity preserved at 80 °C), and in the presence of NaOH and HCl solutions up to a concentration of 12.5 mM and 25 mM, respectively. The hemagglutinating activity of SRL was inhibited by inulin. Cd2+ and Hg2+ ions strongly reduced the hemagglutinating activity at concentrations from 1.25 mM to 10 mM. SRL exhibited anti-proliferative activity toward both hepatoma Hep G2 cells and leukemia L1210 cells, with an IC50 of 7 μM and 19 μM, respectively. The activity of HIV-1 reverse transcriptase could also be inhibited by SRL, with an IC50 of 10 μM. View Full-Text
Keywords: lectin; Stropharia rugosoannulata; hemagglutinating activity; antiproliferative activity lectin; Stropharia rugosoannulata; hemagglutinating activity; antiproliferative activity
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Zhang, W.; Tian, G.; Geng, X.; Zhao, Y.; Ng, T.B.; Zhao, L.; Wang, H. Isolation and Characterization of a Novel Lectin from the Edible Mushroom Stropharia rugosoannulata. Molecules 2014, 19, 19880-19891.

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