Next Article in Journal
Eckol Enhances Heme Oxygenase-1 Expression through Activation of Nrf2/JNK Pathway in HepG2 Cells
Previous Article in Journal
Anti-Fibrosis Effect of Scutellarin via Inhibition of Endothelial–Mesenchymal Transition on Isoprenaline-Induced Myocardial Fibrosis in Rats
Article Menu
Issue 10 (October) cover image

Export Article

Open AccessArticle
Molecules 2014, 19(10), 15624-15637;

Anti-Inflammatory Effect of 3-O-[(6'-O-Palmitoyl)-β-D-glucopyranosyl Sitosterol] from Agave angustifolia on Ear Edema in Mice

Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social (IMSS), Argentina No. 1, Xochitepec, Morelos CP 62790, Mexico
Centro de Desarrollo de Productos Bióticos, Instituto Politécnico Nacional (IPN), Km 6 carr. Yautepec-Jojutla, calle Ceprobi No. 6 col. San Isidro, Yautepec, Morelos CP 62731, Mexico
Facultad de Medicina, Universidad Autónoma del Estado de Morelos, Leñeros s/n. Col. Volcanes, Cuernavaca, Morelos CP 62350, Mexico
Author to whom correspondence should be addressed.
Received: 11 June 2014 / Revised: 11 September 2014 / Accepted: 12 September 2014 / Published: 29 September 2014
(This article belongs to the Section Natural Products Chemistry)
Full-Text   |   PDF [1820 KB, uploaded 29 September 2014]   |  


In Mexico Agave angustifolia has traditionally been used to treat inflammation. The aim of this study was to measure the anti-inflammatory effect of the extract of A. angustifolia, the isolation and identification of active compounds. From the acetone extract two active fractions were obtained, (AsF13 and AaF16). For the characterization of pharmacological activity, the acute inflammatory model of mouse ear edema induced with TPA was used. The tissue exposed to TPA and treatments were subjected to two analysis, cytokine quantification (IL-1β, IL-6, IL-10 and TNF-α) and histopathological evaluation. The active fraction (AaF16) consisted principally of 3-O-[(6'-O-palmitoyl)-β-D-glucopyranpsyl] sitosterol. In AaF13 fraction was identified β-sitosteryl glucoside (2) and stigmasterol (3). The three treatments tested showed a concentration-dependent anti-inflammatory effect (AaAc Emax = 33.10%, EC50 = 0.126 mg/ear; AaF13 Emax = 54.22%, EC50 = 0.0524 mg/ear; AaF16 Emax = 61.01%, EC50 = 0.050 mg/ear). The application of TPA caused a significant increase on level of IL-1β, IL-6 and TNFα compared with basal condition, which was countered by any of the experimental treatments. Moreover, the experimental treatments induced a significant increase in the levels of IL-4 and IL-10, compared to the level observed when stimulated with TPA. Therefore, the anti-inflammatory effect of Agave angustifolia, is associated with the presence of 3-O-[(6'-O-palmitoyl)-β-D-glucopyranosyl] sitosterol. View Full-Text
Keywords: Agave angustifolia; 3-O-[(6'-O-palmitoyl)-β-D-glucopyranosyl] sitosterol; anti-inflammatory Agave angustifolia; 3-O-[(6'-O-palmitoyl)-β-D-glucopyranosyl] sitosterol; anti-inflammatory

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Hernández-Valle, E.; Herrera-Ruiz, M.; Salgado, G.R.; Zamilpa, A.; Ocampo, M.L.A.; Aparicio, A.J.; Tortoriello, J.; Jiménez-Ferrer, E. Anti-Inflammatory Effect of 3-O-[(6'-O-Palmitoyl)-β-D-glucopyranosyl Sitosterol] from Agave angustifolia on Ear Edema in Mice. Molecules 2014, 19, 15624-15637.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top