A New Isoflavonoid from Seeds of Lepidium sativum L. and Its Protective Effect on Hepatotoxicity Induced by Paracetamol in Male Rats
Abstract
:1. Introduction
2. Results and Discussion
2.1. Chemistry
Position | 1 | 2 | 3 | |||
---|---|---|---|---|---|---|
1H | 13C | 1H | 13C | 1H | 13C | |
2 | 8.68s | 153.1 | 8.51s | 153.6 | 8.5s | 153.2 |
3 | - | 124.7 | - | 120.9 | - | 121.7 |
4 | - | 174.5 | - | 180.6 | - | 180.5 |
5 | - | 153.2 | - | 153.6 | - | 157.2 |
6 | - | 137.2 | - | 137.5 | - | 144.2 |
7 | - | 157.3 | - | 157.1 | - | 131.3 |
8 | 6.14s | 96.1 | 6.15s | 94.7 | 6.78s | 107.8 |
9 | - | 151.3 | - | 151.9 | - | 150.3 |
10 | - | 113.2 | - | 113.9 | - | 116.3 |
1' | - | 124.8 | - | 120.9 | - | 120.8 |
2' | 7.52 (d2.0) | 130.1 | - | 149.1 | - | 147.8 |
3' | 6.94 (d8.0) | 114.7 | - | 148.7 | - | 148.2 |
4' | - | 159.3 | 6.75 (d1.99) | 114.3 | 6.76 (d1.95) | 114.5 |
5' | 6.96 (d2,4) | 114.5 | 7.18 (d2.0) | 121.5 | 6.98 (d2.0) | 121.3 |
6' | 7.52 (d2.9) | 130.6 | 6.85 (d1.95) | 122.6 | 7.15 (d1.95) | 122.3 |
1'' | - | - | - | - | 4.82 (d,8.9) | 73.7 |
2'' | - | - | - | - | 3.75 (dd8.9,9.2) | 84.2 |
3'' | - | - | - | - | 3.37 (t8,9) | 72.2 |
4'' | - | - | - | - | 3.41 (t8.9) | 71.3 |
5'' | - | - | - | - | 2.94 (ddd6.2, 8.8) | 84.6 |
6'' | - | - | - | - | 3.81 (d12.3) 3.54 (dd6.2, 12.5) | 62.2 |
OCH3-5 | 3.83s | 62.2 | 3.84s | 61.3 | 3.79s | 61.8 |
OCH3-6 | 3.83s | 61.1 | 3.85s | 62.2 | 3.83s | 60.6 |
OCH3-4' | 3.86s | 55.5 | - | - | - | |
OCH2O-3',4' | - | - | 6.15s | 101.3 | 6.07s | 101.6 |
OH-7 | 5.33s | - | 5.35s | - | - | - |
2.2. Biology Assays
2.2.1. Effect of Compounds 1, 2 and 3 on Liver Function
Group | ALT(U/L) | AST(U/L) | ALP(U/L) | GGT(U/L) |
---|---|---|---|---|
Control Group 1 | 40 ± 1.5 | 43 ± 2.1 | 33 ± 2.4 | 60 ± 1.4 |
Group2: Paracetamol | 70 ± 1.6 * | 80 ± 2.5 * | 71 ± 2.2 * | 117 ± 2.5 * |
Group 3: Paracetamol + compound 1 | 60 ± 1.45 *# | 60 ± 2.2 *# | 53 ± 1.9 *# | 91 ± 2.4 *# |
Group 4: Paracetamol + compound 2 | 61 ± 1.33 *# | 62.2 ± 2.3 *# | 54 ± 1.8 *# | 95 ± 2.7 *# |
Group 5: Paracetamol + compound 3 | 50 ± 1.2 # | 53.5 ± 2.1 # | 39 ± 2.1 # | 72 ± 1.6 # |
2.2.2. Effect of Compounds 1, 2 and 3 on Oxidative Stress Parameters
Group | TAC (mM/L) | MDA nmole/g tissue | SOD units/g tissue | GPx units/g tissue | CAT mole/min/g tissue | GSH μmol/ mg protein | GST units/g tissue |
---|---|---|---|---|---|---|---|
Group 1: Control | 1.2 ± 0.04 | 160 ± 3.23 | 60 ± 1.25 | 150 ± 1.5 | 0.8 ± 0.02 | 60 ± 1.3 | 2.2 ± 0.11 |
Group 2: Paracetamol | 0.52 ± 0.02 * | 547 ± 9.46 * | 24 ± 2.4 * | 112 ± 1.2 * | 0.42 ± 0.01 * | 35 ± 0.85 * | 1.1 ± 0.01 * |
Group 3: Paracetamol + compound 1 | 0.61 ± 0.03 *# | 320 ± 8.5 *# | 30 ± 2.1 *# | 118 ± 1.5 *# | 0.56 ± 0.01 *# | 40 ± 0.65 *# | 1.34 ± 0.01 *# |
Group 4: Paracetamol + compound 2 | 0.65 ± 0.03 *# | 323 ± 9.11 *# | 33 ± 2.2 *# | 115 ± 1.4 *# | 0.55 ± 0.01 *# | 42 ± 0.54 *# | 1.37 ± 0.01 *# |
Group 5: Paracetamol + compound 3 | 0.73 ± 0.02 # | 211 ± 5.22 # | 36 ± 1.5 # | 120 ± 6.5 # | 0.621 ± 0.01 # | 45.5 ± 1.2 # | 1.50 ± 0.8 # |
2.2.3. Histopathology
3. Experimental Section
3.1. General Information
3.2. Plant Material
3.3. Plant Extract
3.4. 7-Hydroxy-4',5,6-trimethoxyisoflavone (1)
3.5. 7-Hydroxy-5,6-dimethoxy-2',3'-methylenedioxyisoflavone (2)
3.6. 5,6-Dimethoxy-2',3'-methylenedioxy-7-C-β-d-glucopyranosylisoflavone (3)
3.7. Animals
3.8. Animal Treatments
3.9. Sample Preparation
3.10. Biochemical Analysis
3.11. Preparation of Tissue Homogenates
3.12. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sakran, M.; Selim, Y.; Zidan, N. A New Isoflavonoid from Seeds of Lepidium sativum L. and Its Protective Effect on Hepatotoxicity Induced by Paracetamol in Male Rats. Molecules 2014, 19, 15440-15451. https://doi.org/10.3390/molecules191015440
Sakran M, Selim Y, Zidan N. A New Isoflavonoid from Seeds of Lepidium sativum L. and Its Protective Effect on Hepatotoxicity Induced by Paracetamol in Male Rats. Molecules. 2014; 19(10):15440-15451. https://doi.org/10.3390/molecules191015440
Chicago/Turabian StyleSakran, Mohamed, Yasser Selim, and Nahla Zidan. 2014. "A New Isoflavonoid from Seeds of Lepidium sativum L. and Its Protective Effect on Hepatotoxicity Induced by Paracetamol in Male Rats" Molecules 19, no. 10: 15440-15451. https://doi.org/10.3390/molecules191015440
APA StyleSakran, M., Selim, Y., & Zidan, N. (2014). A New Isoflavonoid from Seeds of Lepidium sativum L. and Its Protective Effect on Hepatotoxicity Induced by Paracetamol in Male Rats. Molecules, 19(10), 15440-15451. https://doi.org/10.3390/molecules191015440