Chemical Composition and Cytotoxicity Evaluation of Essential Oil from Leaves of Casearia Sylvestris, Its Main Compound α-Zingiberene and Derivatives
Abstract
:1. Introduction
2. Results and Discussion
Compound | KI | % Composition |
---|---|---|
δ-elemene | 1339 | 0.29 |
α-copaene | 1376 | 0.20 |
β-elemene | 1391 | 2.19 |
α-cis-bergamotene | 1415 | 0.24 |
E-caryophyllene | 1418 | 14.27 |
gurjunene | 1432 | 0.16 |
α-guaiene | 1439 | 1.50 |
α-humulene | 1454 | 1.68 |
α-patchoulene | 1456 | 1.08 |
α-acoradiene | 1463 | 4.11 |
β-acoradiene | 1466 | 2.30 |
γ-muurolene | 1477 | 5.16 |
viridiflorene | 1493 | 5.07 |
α-zingiberene | 1495 | 48.31 |
β-trans- guaiene | 1500 | 1.33 |
7-epi-α-selinene | 1517 | 1.05 |
δ-cadinene | 1524 | 1.33 |
germacrene B | 1556 | 0.39 |
khusimone | 1593 | 0.25 |
10-epi-γ-eudesmol | 1619 | 0.35 |
1-epi-cubenol | 1627 | 0.51 |
α-muurolol | 1645 | 3.72 |
α-cadinol | 1653 | 3.24 |
TOTAL | 98.73 |
Sample | A2058 | HeLa | MCF-7 | U-87 | Siha | HL60 | B16F10 Nex2 |
---|---|---|---|---|---|---|---|
Crude oil | 41.1 ± 2.0 | 29.0 ± 0.0 | 42.2 ± 3.4 | 27.1 ± 1.5 | 23.9 ± 0.46 | 12.0 ± 1.9 | 61.5 ± 1.8 |
ZI | >200 | 63.2 ± 2.7 | >200 | 153.0 ± 29.9 | 48.0 ± 0.8 | 98.7 ± 9.7 | 27.0 ± 1.2 |
PHZ | >200 | >200 | >200 | >200 | 160 | >200 | 65.2 ± 1.1 |
THZ | 64.1 ± 3.0 | 65.6 ± 1.4 | 131.3 ± 10.0 | 59.4 ± 3.5 | 54.9 ± 2.8 | 34.3 ± 0.9 | >200 |
Cisplatin (control) | nd | 20.3 ± 1.20 | nd | 44.9 ± 6.0 | 59.8 ± 0.0 | 20.9 ± 1.50 | 52.6 ± 4.49 |
3. Experimental
3.1. Plant Material
3.2. Essential Oil Extraction and Analysis
3.3. Chromatographic Separation Procedures
3.4. Hydrogenation of α-zingiberene
3.5. Cell Lines
3.6. In Vitro Cytotoxic Activity
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Bou, D.D.; Lago, J.H.G.; Figueiredo, C.R.; Matsuo, A.L.; Guadagnin, R.C.; Soares, M.G.; Sartorelli, P. Chemical Composition and Cytotoxicity Evaluation of Essential Oil from Leaves of Casearia Sylvestris, Its Main Compound α-Zingiberene and Derivatives. Molecules 2013, 18, 9477-9487. https://doi.org/10.3390/molecules18089477
Bou DD, Lago JHG, Figueiredo CR, Matsuo AL, Guadagnin RC, Soares MG, Sartorelli P. Chemical Composition and Cytotoxicity Evaluation of Essential Oil from Leaves of Casearia Sylvestris, Its Main Compound α-Zingiberene and Derivatives. Molecules. 2013; 18(8):9477-9487. https://doi.org/10.3390/molecules18089477
Chicago/Turabian StyleBou, Diego Dinis, João Henrique G. Lago, Carlos R. Figueiredo, Alisson L. Matsuo, Rafael C. Guadagnin, Marisi G. Soares, and Patricia Sartorelli. 2013. "Chemical Composition and Cytotoxicity Evaluation of Essential Oil from Leaves of Casearia Sylvestris, Its Main Compound α-Zingiberene and Derivatives" Molecules 18, no. 8: 9477-9487. https://doi.org/10.3390/molecules18089477