Inhibition of Lipopolysaccharide-Induced Proinflammatory Responses by Buddleja officinalis Extract in BV-2 Microglial Cells via Negative Regulation of NF-kB and ERK1/2 Signaling
Abstract
:1. Introduction
2. Results and Discussion
2.1. BOWE Attenuates LPS-Induced NO Production
2.2. BOWE Inhibits LPS-Induced iNOS Expression, but not COX-2 Expression
2.3. BOWE Inhibits LPS-Induced IL-1β and IL-6, but not TNF-α
2.4. BOWE Inhibits LPS-Induced NF-κB Signaling
2.5. BOWE Inhibits LPS-Induced ERK1/2 Phosphorylation, but not p38 and JNK Phosphorylation
3. Experimental
3.1. Preparation of B. officinalis Water Extract
3.2. Cell Culture
3.3. Cell Viability and NO Assay
3.4. Enzyme-Linked Immunosorbent Assay (ELISA)
3.5. RNA Isolation and Reverse Transcription-Polymerase Chain Reaction (RT-PCR)
iNOS [29] | sense | CCG TCC ACA GTA TGT GAG GA |
anti-sense | GAA CTC CAA GGT GGC AGC A | |
COX-2 [30] | sense | TGA TGA CTG CCC AAC TCC CAT G |
anti-sense | AAT GTT GAA GGT GTC CGG CAG C | |
TNF-α [30] | sense | CAG ACC CTC ACA CTC AGA TCA TCT T |
anti-sense | CAG AGC AAT GAC TCC AAA GTA GAC CT | |
IL-1β [31] | sense | GAG GCT GAC AGA CCC CAA AAG AT |
anti-sense | GCA CGA GGC ATT TTT GTT GTT CA | |
IL-6 [22] | sense | GGA GCC CAC CAA GAA CGA TAG TCA |
anti-sense | GAA GTA GGG AAG GCC GTG GTT |
3.6. Western Blot Analysis of c-Jun, MAPKs, and NF-κB Protein Expression
3.7. Statistical Analysis
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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- Sample Availability: Not available.
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Oh, W.-J.; Jung, U.; Eom, H.-S.; Shin, H.-J.; Park, H.-R. Inhibition of Lipopolysaccharide-Induced Proinflammatory Responses by Buddleja officinalis Extract in BV-2 Microglial Cells via Negative Regulation of NF-kB and ERK1/2 Signaling. Molecules 2013, 18, 9195-9206. https://doi.org/10.3390/molecules18089195
Oh W-J, Jung U, Eom H-S, Shin H-J, Park H-R. Inhibition of Lipopolysaccharide-Induced Proinflammatory Responses by Buddleja officinalis Extract in BV-2 Microglial Cells via Negative Regulation of NF-kB and ERK1/2 Signaling. Molecules. 2013; 18(8):9195-9206. https://doi.org/10.3390/molecules18089195
Chicago/Turabian StyleOh, Won-Jun, Uhee Jung, Hyun-Soo Eom, Hee-June Shin, and Hae-Ran Park. 2013. "Inhibition of Lipopolysaccharide-Induced Proinflammatory Responses by Buddleja officinalis Extract in BV-2 Microglial Cells via Negative Regulation of NF-kB and ERK1/2 Signaling" Molecules 18, no. 8: 9195-9206. https://doi.org/10.3390/molecules18089195