Design, Synthesis and Evaluation of Dual-Modality Glyco-Nanoparticles for Tumor Imaging
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis and Characterization of CCPM Nanoparticles
Specification | NIRF-CCPM | DTPA-NIRF-CCPM | DG-CCPM |
---|---|---|---|
Particles (nm) a | 24 ± 8.9 | 24 ± 8.9 | 24 ± 8.9 |
Zeta potential (mV) b | +1.2 | −5.83 ± 2.40 | −5.83 ± 1.08 |
Dyes/Particle c | 21 | 21 | 21 |
DTPA/Particle d | 0 | 19 | 19 |
Amines/Particle e | 180 | 160 | 145 |
Concentration f | 1.54 × 1015 | 7 × 1014 | 3.4 × 1014 |
DG/Particle g | N/A | N/A | 15 |
2.2. Radiolabeling of the Nanoparticles
2.3. Microscopic Imaging of DTPA-NIRF-CCPM-DG
2.4. Biodistribution in Tumor Bearing mice
2.5. In Vivo Gamma Imaging
2.6. In vivo NIRF Imaging
3. Experimental
3.1. General
3.2. Synthesis and Characterization of DG-CCPM
3.2.1. Synthesis of d-Glucosamine-NH-SA
3.2.2. Conjugation of DG-COOH to CCPM
3.2.3. Standard Curves of DTPA-NIRF-CCPM
3.3. Radiolabeling
3.4. Fluorescence Microscopy Study
3.5. Biodistribution Studies
3.6. In Vivo Gamma Imaging
3.7. In Vivo NIRF Imaging
3.8. Statistical Analysis
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Zhu, H.; Zhao, J.; Lin, X.; Hong, Y.; Li, C.; Yang, Z. Design, Synthesis and Evaluation of Dual-Modality Glyco-Nanoparticles for Tumor Imaging. Molecules 2013, 18, 6425-6438. https://doi.org/10.3390/molecules18066425
Zhu H, Zhao J, Lin X, Hong Y, Li C, Yang Z. Design, Synthesis and Evaluation of Dual-Modality Glyco-Nanoparticles for Tumor Imaging. Molecules. 2013; 18(6):6425-6438. https://doi.org/10.3390/molecules18066425
Chicago/Turabian StyleZhu, Hua, Jun Zhao, Xinfeng Lin, Ye Hong, Chun Li, and Zhi Yang. 2013. "Design, Synthesis and Evaluation of Dual-Modality Glyco-Nanoparticles for Tumor Imaging" Molecules 18, no. 6: 6425-6438. https://doi.org/10.3390/molecules18066425