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Molecules 2012, 17(6), 7083-7092; https://doi.org/10.3390/molecules17067083

Astragalus Polysaccharide Improves Palmitate-Induced Insulin Resistance by Inhibiting PTP1B and NF-κB in C2C12 Myotubes

Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China
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Author to whom correspondence should be addressed.
Received: 21 May 2012 / Revised: 30 May 2012 / Accepted: 6 June 2012 / Published: 11 June 2012
(This article belongs to the Section Natural Products Chemistry)
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Abstract

We investigated the effects of Astragalus polysaccharide (APS) on palmitate-induced insulin resistance in C2C12 skeletal muscle myotubes. Palmitate-reduced glucose uptake was restored by APS. APS prevented palmitate-induced C2C12 myotubes from impaired insulin signaling by inhibiting Ser307 phosphorylation of insulin receptor substrate-1 (IRS-1) and increasing Ser473 phosphorylation of Akt. Moreover, the increases in protein-tyrosine phosphatase-1B (PTP1B) protein level and NF-κB activation associated with palmitate treatment were also prevented by APS. However the treatment with APS didn’t change AMP-activated protein kinase (AMPK) activation in palmitate-induced myotubes. The results of the present study suggest that Astragalus polysaccharide inhibits palmitate-induced insulin resistance in C2C12 myotubes by inhibiting expression of PTP1B and regulating NF-κB but not AMPK pathway. View Full-Text
Keywords: insulin resistance; NF-κB; PTP1B; Astragalus polysaccharide insulin resistance; NF-κB; PTP1B; Astragalus polysaccharide
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Zhao, M.; Zhang, Z.-F.; Ding, Y.; Wang, J.-B.; Li, Y. Astragalus Polysaccharide Improves Palmitate-Induced Insulin Resistance by Inhibiting PTP1B and NF-κB in C2C12 Myotubes. Molecules 2012, 17, 7083-7092.

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