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Reversible Low-Light Induced Photoswitching of Crowned Spiropyran-DO3A Complexed with Gadolinium(III) Ions

1
Department of Biomedical Engineering, University of California, Davis, CA 95616, USA
2
Department of Electrical and Computer Engineering, University of California, Davis, CA 95616, USA
3
Department of Cell and Tissue Biology, University of California, San Francisco, CA 94143, USA
*
Author to whom correspondence should be addressed.
Molecules 2012, 17(6), 6605-6624; https://doi.org/10.3390/molecules17066605
Received: 26 March 2012 / Revised: 11 May 2012 / Accepted: 25 May 2012 / Published: 31 May 2012
Photoswitchable spiropyran has been conjugated to the crowned ring system DO3A, which improves its solubility in dipolar and polar media and stabilizes the merocyanine isomer. Adding the lanthanide ion gadolinium(III) to the macrocyclic ring system leads to a photoresponsive magnetic resonance imaging contrast agent that displays an increased spin-lattice relaxation time (T1) upon visible light stimulation. In this work, the photoresponse of this photochromic molecule to weak light illumination using blue and green light emitting diodes was investigated, simulating the emission spectra from bioluminescent enzymes. Photon emission rate of the light emitting diodes was changed, from 1.75 × 1016 photons·s−1 to 2.37 × 1012 photons·s−1. We observed a consistent visible light-induced isomerization of the merocyanine to the spiropyran form with photon fluxes as low as 2.37 × 1012 photons·s−1 resulting in a relaxivity change of the compound. This demonstrates the potential for use of the described imaging probes in low light level applications such as sensing bioluminescence enzyme activity. The isomerization behavior of gadolinium(III)-ion complexed and non-complexed spiropyran-DO3A was analyzed in water and ethanol solution in response to low light illumination and compared to the emitted photon emission rate from over-expressed Gaussia princeps luciferase. View Full-Text
Keywords: spiropyran; photochromic molecule; DO3A; MRI contrast agent; luciferase; light emitting diodes; rate constant; low light applications spiropyran; photochromic molecule; DO3A; MRI contrast agent; luciferase; light emitting diodes; rate constant; low light applications
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Kruttwig, K.; Yankelevich, D.R.; Brueggemann, C.; Tu, C.; L’Etoile, N.; Knoesen, A.; Louie, A.Y. Reversible Low-Light Induced Photoswitching of Crowned Spiropyran-DO3A Complexed with Gadolinium(III) Ions. Molecules 2012, 17, 6605-6624.

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